Ki67/MKI67 Antibody

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-89719] - Ki67 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti-KI67 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This antibody generated a specific nuclear staining in epithelial cells and the staining was more intense in the cells close to the bases of crypts. Weak to moderate positivity was found at the cytoplasmic level also.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - Effect of saireito on the suppression of cell proliferation induced by 5-fluorouracil (5-FU) in mouse small intestines. 5-FU (50 mg/kg) was injected i.p. while saireito (1000 mg/kg) was administered p.o. twice, 30 min before and 8 h after 5-FU injection. The jejunum was excised, sectioned, and Ki67 immunostaining was performed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116213), licensed under a CC-BY licence.

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Detection of Ki67 in formalin-fixed paraffin embedded mouse intestine using NB110-89719.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Ki-67 (NB110-89719) used at a dilution of 1:250.

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Immunohistochemical analysis of mouse spleen.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - IHC analysis of Ki67 in mouse cornea. Image courtesy of product review by Bo-Yie Chen.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Ki67/MKI67 Antibody Summary

• Immunogen: The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Mouse KI67/MKI67, between aminoacids 2000-2050 (2021-2036) Uniprot# E9PVX6.
• Localization: Nuclear
• Marker: Proliferation Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MKI67
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
• Application Notes: Ki67/MKI67 Antibody was used for IHC-Fr (PMID: 23419702), WB (PMID: 23643677), and Flow (PMID 25733567).
• Theoretical MW: 351 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Ki67/MKI67 Knockout HeLa Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31286466).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ki67/MKI67 Antibody

• antigen identified by monoclonal Ki-67
• Antigen Ki67
• antigen KI-67
• Ki67
• Ki-67
• KIA
• Marker Of Proliferation Ki-67
• MIB-
• MIB-1
• MKI67
• PPP1R105
• Proliferation Marker Protein Ki-67
• proliferation-related Ki-67 antigen
• Protein Phosphatase 1
• Regulatory Subunit 105
• TSG126

Background

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ki67/MKI67 Antibody (NB110-89719)(22)

Publications using NB110-89719

• Lemons K, Fu Z, Ogura T, Lin W TRPM5-expressing Microvillous Cells Regulate Region-specific Cell Proliferation and Apoptosis During Chemical Exposure Neuroscience Mar 26 2020 [PMID: 32224228] (IHC, Mouse)
• Kanatsu-Shinohara M, Chen G, Morimoto H, Shinohara T CD2 is a surface marker for mouse and rat spermatogonial stem cells J. Reprod. Dev. Mar 26 2020 [PMID: 32213736] (IHC, Mouse)
• Quach C, Song Y, Guo H, et al. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice Nat Commun Dec 12 2019 [PMID: 31831743] (IHC, Mouse)
• Wangler S, Peroglio M, Menzel U et al. Mesenchymal Stem Cell Homing Into Intervertebral Discs Enhances the Tie2-positive Progenitor Cell Population, Prevents Cell Death, and Induces a Proliferative Response Spine Dec 1 2019 [PMID: 31730570] (IHC, Human)
• Shinohara T, Kazuki K, Ogonuki N et al. Transfer of a Mouse Artificial Chromosome into Spermatogonial Stem Cells Generates Transchromosomic Mice Stem Cell Reports 2017 Oct 10 [PMID: 28943251] (Flow, Mouse)
• Hill AA, Kim M, Ruiz DFZ et al. Defective intestinal repair after short-term high fat diet due to loss of efferocytosis bioRxiv 2019 Oct 02 (ICC/IF, Mouse)
• Liberia T, Martin-Lopez E, Meller SJ, Greer CA Sequential maturation of olfactory sensory neurons in the mature olfactory epithelium eNeuro Sep 25 2019 [PMID: 31554664] (ICC/IF, Mouse)
• Akiyama N, Yamamoto-Fukuda T, Yoshikawa M, Kojima H, Regulation of DNA methylation levels in the process of oral mucosal regeneration in a rat oral ulcer model Histol. Histopathol. Jul 9 2019 [PMID: 31286466] (Rat)
• Dang, TC;Ishii, Y;Nguyen, V;Yamamoto, S;Hamashima, T;Okuno, N;Nguyen, QL;Sang, Y;Ohkawa, N;Saitoh, Y;Shehata, M;Takakura, N;Fujimori, T;Inokuchi, K;Mori, H;Andrae, J;Betsholtz, C;Sasahara, M; Powerful Homeostatic Control of Oligodendroglial Lineage by PDGFR alpha in Adult Brain Cell Rep Apr 23 2019 [PMID: 31018125] (IHC-Fr, Mouse)
• Kanter JE, Kramer F, Barnhart S et al. A Novel Strategy to Prevent Advanced Atherosclerosis and Lower Blood Glucose in a Mouse Model of Metabolic Syndrome. Diabetes. Feb 26 2018 [PMID: 29483182] (Mouse)
• Yoon J, Leyva-Castillo JM, Wang G et al. IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization. J. Exp. Med. Aug 22 2016 [PMID: 27551155] (IHC, Mouse)
• Liou JC, Teng MC, Tsai YS et al. UV-blocking spectacle lens protects against UV-induced decline of visual performance. Mol Vis 2015 [PMID: 26283865]
• Gkouskou KK, Ioannou M, Pavlopoulos GA et al. Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis. Oncogene. 2015 Aug 17 [PMID: 26279300] (IHC, Mouse)
• He S, Zhao Z, Yang Y et al. Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nat Commun. 2015 Aug 03 [PMID: 26234763] (IHC-P, Mouse)
• Rodriguez M, Siwko S, Zeng L et al. Prostate-specific G-protein-coupled receptor collaborates with loss of PTEN to promote prostate cancer progression. Oncogene 2015 Jun 1 [PMID: 26028029]
• Talaber G, Tuckermann JP, Okret S. ACTH controls thymocyte homeostasis independent of glucocorticoids FASEB J. 2015 Mar 02 [PMID: 25733567] (FLOW, Mouse)
• Kato S, Hayashi S, Kitahara Y et al. Saireito (TJ-114), a Japanese Traditional Herbal Medicine, Reduces 5-Fluorouracil-Induced Intestinal Mucositis in Mice by Inhibiting Cytokine-Mediated Apoptosis in Intestinal Crypt Cells PLoS ONE. 2015 Jan 08 [PMID: 25565296] (IHC-P, Human)
  
  Details:
  Ki-67/MKI67 antibody used for IHC-P on murine jejunum tissue sections in experiments involving the modulatory effects of a Japanese Traditional Herbal Medicine called Saireito on 5-Fluorouracil-Induced Intestinal Mucositis in Mice. IHC-P Assay involved - 10% neutralized formalin fixation of tissues, paraffin embedding followed by 4um sectioning, antigen retrieval with HistoVT One solution/90C for 20 minutes in water bath, endogenous peroxidases blocked with 3%H2O2, primary detection with Vectastain Elite ABC rabbit IgG kit followed by counter-staining with hematoxylin (Figure 6).
• Rodriguez M, Luo W, Weng J et al. PSGR promotes prostatic intraepithelial neoplasia and prostate cancer xenograft growth through NF-kB. Oncogenesis. 2014 Aug 12 [PMID: 25111863] (IHC-P, Mouse)
• Ifkovits JL, Addis RC, Epstein JA. Inhibition of TGFB Signaling Increases Direct Conversion of Fibroblasts to Induced Cardiomyocytes. PLoS ONE 2014 Mar 3 [PMID: 24586958] (ICC/IF, Mouse)
• Lin DP, Chang HH, Yang LC et al. Assessment of ultraviolet B-blocking effects of weekly disposable contact lenses on corneal surface in a Mouse model. Mol Vis 2013 May 29 [PMID: 23734085] (IHC-P, Mouse)
• Jo M, Yun HM, Park KR et al. Lung tumor growth promoting function of peroxiredoxin 6. Free Radic Biol Med 2013 May 2 [PMID: 23643677] (WB, IHC-P, Mouse)
• Mobley AS, Bryant AK, Richard MB et al. Age-dependent regional changes in the rostral migratory stream. Neurobiol Aging 2013 Feb 15 [PMID: 23419702] (IHC-Fr, ICC/IF, Mouse)

Reviews for Ki67/MKI67 Antibody (NB110-89719) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Anonymous IHC-P Mouse 11/30/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Skeletal muscle tissue
• Species: Mouse
• Lot: 050806

Comments

• Comments: Heat mediated low pH antigen retrieval was performed on sections prior to use. Permeabilization with 0.1% triton. The antibody was diluted 1/200. An Alexa Fluor anti-rabbit 488 was used to detect Ki67 antibody.

reviewed by: James Xiao IHC-P Mouse 04/07/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse lung tissue that was exposed to cigarette smoke
• Species: Mouse

reviewed by: Anonymous IHC-P Mouse 02/06/2012

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse cornea
• Species: Mouse

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: Goat, Secondary Ab Dilution: 1:500

Details

• Fixation Details: Sample Preparation: Paraffin Section Method

FAQs for Ki67/MKI67 Antibody (NB110-89719). (Showing 1 - 1 of 1 FAQs).

1. Is there a clone number for this Ki67 antibody?
   • This antibody is polyclonal, and thus it does not have a clone number.

Bioinformatics

• Gene Symbol: MKI67
• Entrez:
  • 4288(Human)
  • 17345(Mouse)
• Uniprot:
  • P46013(Human)