Ki67/MKI67 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody [NB110-89719] - Ki67 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti-KI67 antibody. The staining was ...read more
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - Effect of saireito on the suppression of cell proliferation induced by 5-fluorouracil (5-FU) in mouse small intestines. 5-FU (50 mg/kg) was injected ...read more
Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Detection of Ki67 in formalin-fixed paraffin embedded mouse intestine using NB110-89719.
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Ki-67 (NB110-89719) used at a dilution of 1:250.
Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Immunohistochemical analysis of mouse spleen.
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - IHC analysis of Ki67 in mouse cornea. Image courtesy of product review by Bo-Yie Chen.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Ki67/MKI67 Antibody Summary

Immunogen
The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Mouse KI67/MKI67, between aminoacids 2000-2050 (2021-2036) Uniprot# E9PVX6.
Localization
Nuclear
Marker
Proliferation Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MKI67
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
Ki67/MKI67 Antibody was used for IHC-Fr (PMID: 23419702), WB (PMID: 23643677), and Flow (PMID 25733567).
Theoretical MW
351 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Ki67/MKI67 Knockout HeLa Cell Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB110-89719 in the following applications:

Publications
Read Publications using
NB110-89719 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31286466).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ki67/MKI67 Antibody

  • antigen identified by monoclonal Ki-67
  • Antigen Ki67
  • antigen KI-67
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-
  • MIB-1
  • MKI67
  • PPP1R105
  • Proliferation Marker Protein Ki-67
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ki67/MKI67 Antibody (NB110-89719)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(1)
Flow
(1)
ICC/IF
(4)
IHC
(6)
IHC-Fr
(2)
IHC-P
(5)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(17)
Rat
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB110-89719 Applications Species
Lemons K, Fu Z, Ogura T, Lin W TRPM5-expressing Microvillous Cells Regulate Region-specific Cell Proliferation and Apoptosis During Chemical Exposure Neuroscience Mar 26 2020 [PMID: 32224228] (IHC, Mouse) IHC Mouse
Kanatsu-Shinohara M, Chen G, Morimoto H, Shinohara T CD2 is a surface marker for mouse and rat spermatogonial stem cells J. Reprod. Dev. Mar 26 2020 [PMID: 32213736] (IHC, Mouse) IHC Mouse
Quach C, Song Y, Guo H, et al. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice Nat Commun Dec 12 2019 [PMID: 31831743] (IHC, Mouse) IHC Mouse
Wangler S, Peroglio M, Menzel U et al. Mesenchymal Stem Cell Homing Into Intervertebral Discs Enhances the Tie2-positive Progenitor Cell Population, Prevents Cell Death, and Induces a Proliferative Response Spine Dec 1 2019 [PMID: 31730570] (IHC, Human) IHC Human
Shinohara T, Kazuki K, Ogonuki N et al. Transfer of a Mouse Artificial Chromosome into Spermatogonial Stem Cells Generates Transchromosomic Mice Stem Cell Reports 2017 Oct 10 [PMID: 28943251] (Flow, Mouse) Flow Mouse
Hill AA, Kim M, Ruiz DFZ et al. Defective intestinal repair after short-term high fat diet due to loss of efferocytosis bioRxiv 2019 Oct 02 (ICC/IF, Mouse) ICC/IF Mouse
Liberia T, Martin-Lopez E, Meller SJ, Greer CA Sequential maturation of olfactory sensory neurons in the mature olfactory epithelium eNeuro Sep 25 2019 [PMID: 31554664] (ICC/IF, Mouse) ICC/IF Mouse
Akiyama N, Yamamoto-Fukuda T, Yoshikawa M, Kojima H, Regulation of DNA methylation levels in the process of oral mucosal regeneration in a rat oral ulcer model Histol. Histopathol. Jul 9 2019 [PMID: 31286466] (Rat) Rat
Dang, TC;Ishii, Y;Nguyen, V;Yamamoto, S;Hamashima, T;Okuno, N;Nguyen, QL;Sang, Y;Ohkawa, N;Saitoh, Y;Shehata, M;Takakura, N;Fujimori, T;Inokuchi, K;Mori, H;Andrae, J;Betsholtz, C;Sasahara, M; Powerful Homeostatic Control of Oligodendroglial Lineage by PDGFR alpha in Adult Brain Cell Rep Apr 23 2019 [PMID: 31018125] (IHC-Fr, Mouse) IHC-Fr Mouse
Kanter JE, Kramer F, Barnhart S et al. A Novel Strategy to Prevent Advanced Atherosclerosis and Lower Blood Glucose in a Mouse Model of Metabolic Syndrome. Diabetes. Feb 26 2018 [PMID: 29483182] (Mouse) Mouse
Show All 22 Publications.

Reviews for Ki67/MKI67 Antibody (NB110-89719) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB110-89719:
Filter by Applications
IHC-P
(3)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Ki67/MKI67 NB110-89719
Enlarge
5
reviewed by:
Anonymous
IHC-P Mouse 11/30/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSkeletal muscle tissue
SpeciesMouse
Lot050806

Comments

CommentsHeat mediated low pH antigen retrieval was performed on sections prior to use. Permeabilization with 0.1% triton. The antibody was diluted 1/200. An Alexa Fluor anti-rabbit 488 was used to detect Ki67 antibody.
  5
reviewed by:
James Xiao
IHC-P Mouse 04/07/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse lung tissue that was exposed to cigarette smoke
SpeciesMouse
Immunohistochemistry-Paraffin Ki67/MKI67 NB110-89719
Enlarge
5
reviewed by:
Anonymous
IHC-P Mouse 02/06/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse cornea
SpeciesMouse

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat, Secondary Ab Dilution: 1:500

Details

Fixation DetailsSample Preparation: Paraffin Section Method

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ki67/MKI67 Antibody (NB110-89719). (Showing 1 - 1 of 1 FAQs).

  1. Is there a clone number for this Ki67 antibody?
    • This antibody is polyclonal, and thus it does not have a clone number.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin NB110-89719APC
Biotin NB110-89719B
DyLight 350 NB110-89719UV
DyLight 488 NB110-89719G
DyLight 550 NB110-89719R
DyLight 650 NB110-89719C
DyLight 680 NB110-89719FR
DyLight 755 NB110-89719IR
FITC NB110-89719F
HRP NB110-89719H
PE NB110-89719PE
PerCP NB110-89719PCP

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Blogs on Ki67/MKI67.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Ki67 - an established marker for labelling proliferating cells
Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v...  Read full blog post.

Ki67 - A Crucial Cellular Proliferation Marker
The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line....  Read full blog post.

The Ki67 Antibody in Cell Marker Studies
The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Anonymous
11/30/2016
Application: IHC-P
Species: Mouse

 
James Xiao
04/07/2016
Application: IHC-P
Species: Mouse

Anonymous
02/06/2012
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol MKI67
Entrez
Uniprot