Ki67/MKI67 Antibody (SP6) - Unpurified

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - T47D cells form functional 3D spheroids in a scaffold-free system. Sections of 3D microtissues grown for 12 days were incubated with DAPI and an antibody against Ki67 (green) to stain proliferative cells analyzed by fluorescence microscopy. Nuclei were visualized with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/cam4.630), licensed under a CC-BY license.

Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - PW-12 is efficacious in an orthotopic flank tumor model of MYCN-amplified medulloblastoma. Mice were implanted with 6 x 106 SmoWT medulloblastoma cells in the hindflank. Animals were treated for 7 days with 25 mg/kg of PW-12 (n = 5) or vehicle alone (n = 5). Histology on H&E stain is consistent with medulloblastoma (H&E, 20x). Tumor cell proliferation (Ki67, 40x) was decreased, apoptosis (cleaved-caspase-3, 40x) increased, MYCN (20x) expression downregulated, and vascularity decreased in PW-12 treated tumors as confirmed by histology and cell quantification for apoptosis and cell proliferation. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/Cancer_Molecular_Targets_and_Therapeutics/10.3389/fonc.2015.00111/abstract), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Ki-67/MKI67 Antibody (SP6) [NB600-1252] - Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody.

Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Paraffin-embedded alcohol fixed rat spleen tissue (20x). Antigen retrieval pH 9. Ki67 dilution 1:100 incubation ON 4C. This image was submitted via customer review.

Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - PW-12 is efficacious in a GEM model of MYCN-amplified neuroblastoma. Tumor-bearing TH-MYCN mice were treated for 14 days with either 25 mg/kg of PW-12 (n = 3) or vehicle alone (n = 3). Histology for Ki67 and c-Caspase 3 shows increase in apoptosis and decrease in proliferation in treated tumors. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/Cancer_Molecular_Targets_and_Therapeutics/10.3389/fonc.2015.00111/abstract), licensed under a CC-BY license.

Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Ki-67/MKI67 Antibody (SP6) [NB600-1252] - Ki67 staining in mouse thyroid tissue at pre-tumor stage (green). Dilution is 1:100. This image was submitted via customer Review.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Ki-67 (NB600-1252) immunoreactivity in an FFPE section of mouse small intestine. Primary antibody was diluted 1:100 and left on sections for 1h at room temperature. Secondary antibody was Horse Anti-Rabbit HRP. Image from verified customer review.

Immunohistochemistry of tissue slices treated with trastuzumab and docetaxel. (Ki-67, arrows; A-C) and (cleaved caspase-3, arrows; D-F) were quantified by counting the number of positively stained cells in 4 fields of view at x40. Images are representative of untreated (A, D), trastuzumab at 100 µg/ml (B and E) and docetaxel at 100 nM (C and F). Images were digitally scanned ×40 (AlexaSoft X-PRO).

Immunohistochemistry-Paraffin: Rabbit Monoclonal Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Image depicting Ki67/MKI67 immunoreactivity in a FFPE section of human tonsil. NB600-1252 was diluted 1-100 and left on tissue sections for 30m at room temperature. Secondary was horse anti rabbit HRP polymer. Image from a verified customer review.

Immunohistochemistry-Paraffin: Rabbit Monoclonal Ki67/MKI67 Antibody (SP6) - Unpurified [IMGENEX: IMG-80336] [NB600-1252] - Images depicting Ki67/MKI67 immunoreactivity in FFPE sections of canine cecum and skin. NB600-1252 was diluted 1-100 and left on tissue sections for 30m at room temperature. Image from a verified customer review.

Immunohistochemistry-Paraffin: Rabbit Monoclonal Ki67/MKI67 Antibody (SP6) - Unpurified - Images depicting Ki67/MKI67 immunoreactivity in FFPE sections of equine intestine and spleen. NB600-1252 was diluted 1-100 and left on tissue sections for 30m at room temperature. Image from a verified customer review.

(A) Representative images of immunohistological staining (brown) of SOX2+ olfactory receptor neurons (ORN) progenitor cells, Gap43+ immature ORNs, Ki67+ proliferating cells & cleaved caspase-3+ (Cas3+) apoptotic cells. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Arrowheads indicate Cas3+ apoptotic cells in the OE (n = 6). (B) Numbers of SOX2+ ORN progenitors & Ki67+ actively proliferating cells per mm of the basal layer, & Gap43+ immature ORNs & Cas3+ apoptotic cells per mm of the OE in saline or CSS-treated mice. Data represent means ± SEM (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001 compared with saline-treated mice (one-way ANOVA). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29950987), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Immunohistochemistry of tissue slices treated with trastuzumab & docetaxel. (Ki-67, arrows; A-C) & (cleaved caspase-3, arrows; D-F) were quantified by counting the number of positively stained cells in 4 fields of view at x40. Images are representative of untreated (A, D), trastuzumab at 100 µg/ml (B & E) & docetaxel at 100 nM (C & F). Images were digitally scanned ×40 (AlexaSoft X-PRO). Image collected & cropped by CiteAb from the following publication (//www.spandidos-publications.com/10.3892/or.2015.4074), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - (A–C) Inflammatory cell infiltration & cell division in the nasal RM were evaluated using immunohistochemical staining (brown). Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Representative immunohistochemical images of neutrophils (A), CD3+ lymphocytes (B), & Ki67+ dividing cells (C) (400× magnification), & comparative charts of cell counts in each group. *P < 0.01; **P < 0.01; ****P < 0.0001 (n = 6, one-way analysis of variance). OVA, ovalbumin; CSS, cigarette smoke solution. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32132898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - In vivo anti-tumor effect of Y-TR1 in the NOD/SCID mouse xenograft model using CD26 positive MM cell line JMN. (A) Y-TR1 administered intraperitoneally 4 mg/kg/dose, three times per week, for a total of nine doses from day zero of subcutaneous inoculation of 1 × 107 JMN cells. Average estimated tumor volume on day 55 compared among three groups (control, YS110, Y-TR1, n = 10) w/ Fisher’s protected least protected difference multiple comparison test. Mean tumor volume of the Y-TR1 group significantly lower (* p < 0.05) than that of the control or YS110 group. Mean tumor volume of the YS110 group not significantly altered compared w/ the control. An experiment out of two w/ similar results is shown; (B) Y-TR1 administered intraperitoneally 8 mg/kg/dose, three times per week, for a total of nine doses. The average estimated tumor weight on day 42 compared among three groups (control, 14D10, YS110, Y-TR1, n = 10) w/ Fisher’s protected least protected difference multiple comparison test. Mean tumor weight of the YS110 or Y-TR1 groups significantly lower (* p < 0.05 or ** p < 0.025, respectively) than that of the control group. Mean tumor weight of the Y-TR1 group significantly lower (* p < 0.05) than that of the YS110 group. An experiment out of two w/ similar results is shown; (C) histological analysis of xenograft tumors of JMN cells. JMN-derived tumors show histopathology of sarcomatoid mesothelioma. (×20). a: Hematoxylin & eosin staining. b: Immunohistochemical staining w/ anti-human CD26 antibody revealed CD26 expression in tumor cells. c–e: MIB-1 (Ki67) staining showed a decreased number of MIB-1-positive cells in Y-TR1-treated tumors compared to IgG1- or YS110-treated tumors. Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31398954), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells (A), SOX2+ ORN progenitor cells (B), GAP43+ immature ORNs (C), Ki67+ proliferating cells (D), & cleaved Cas3+ apoptotic cells (E). Each cell except for many OMP+ cells is indicated by arrows. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Numbers of SOX2+ ORN progenitors & Ki67+ actively proliferating cells per mm of the basal layer & OMP+ mature ORNs, GAP43+ immature ORNs, & Cas3+ apoptotic cells per mm of the OE in saline or rhIGF-1-treated mice. Open circles, rectangles, & triangles represent the values for each mouse in the saline, low-IGF-1, & high-IGF-1 treated groups (each n = 6), respectively. The horizontal lines represent the mean value for each group. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; & ∗∗∗∗P < 0.0001 (one-way ANOVA). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30515092), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - (A) Serum immunoglobin E (IgE) levels of the control & cigarette smoke solution (CSS)-treated mice were determined by enzyme linked immunosorbent assay. (B) Representative images of Sirius red staining for eosinophils & periodic acid-Schiff & Alcian blue (PAS/AB) staining for goblet cells in the nasal RM of the control mice & mice treated with 10 doses of CSS (CSS 10). (C) Representative immunohistochemical images of neutrophils & Ki67+ dividing cells in the nasal RM (400× magnification), & comparative charts of Ki67+ cell counts (n = 6, Mann–Whitney U test). (D) Representative images of olfactory marker protein (OMP)+ mature olfactory receptor neurons (ORNs) in two different areas of the olfactory epithelium: the nasal septum & upper lateral area. There were no significant differences in the number of OMP+ mature ORNs between the control & CSS-treated mice (n = 6, Mann–Whitney U test). OVA, ovalbumin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32132898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - (A,B) Representative images of hematoxylin & eosin (H&E)-stained sections of the olfactory epithelium from young adult mice (A, 40× magnification; B, 400× magnification). A black line in (A) indicates the range for counting the number of each cell type. The box in (A) indicates the region of the olfactory epithelium shown at a representative higher magnification in (B). Differences in the number of OMP+ mature olfactory receptor neurons (ORNs) (C), SOX2+ ORN progenitors (D), Ki-67+ proliferating cells (E), GAP43+ immature ORNs (F), & cleaved Cas3+ apoptotic cells (G) in the OE were evaluated by immunohistological staining (brown). Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Representative images (400× magnification) of tissues stained with antibodies against olfactory marker protein (OMP), SRY (sex determining region Y)-box 2 (SOX2), Ki-67 (antigen identified by monoclonal antibody Ki-67), growth associated protein 43 (GAP43), & cleaved caspase 3 (CAS3) are shown. The number of cells per mm of basal layer length (C–G) was counted manually. Data represent the mean ± SD. **P < 0.01 (n = 6, Mann–Whitney U-test). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnagi.2018.00086/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ca, Eq
• Applications: WB, Func, ICC/IF, IHC, IHC, KD
• Clone: SP6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Unpurified

Ki67/MKI67 Antibody (SP6) - Unpurified Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The immunogen for this unpurified KI67/MKI67 Antibody (SP6) was made using a synthetic peptide from the C-Terminus of Human KI67/MKI67.
• Epitope: C-terminus
• Localization: Nuclear
• Marker: Proliferation Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MKI67
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:25-1:50
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:50
  • In vivo assay
  • Knockdown Validated
  • Western Blot
• Application Notes: Use in IHC-WHMT reported in scientific literature (PMID:35104247) IHC-P: recommended incubation time of 30-60 min at RT. Ki67/MKI67 Antibody (SP6) was used for ICC/IF (PMID: 20235278) and IHC-Fr reported in scientific literature (PMID: 23300752). Use in Western blot reported in scientific literature (PMID: 31078687). Use In vivo reported in scientific literature (PMID:31398954)..
• Theoretical MW: 359 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Tonsil Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32731460). Ki67/MKI67 (SP6). antibody reacted with Mouse (PMID: 22020958). and Rat (PMID: 30810241). Equine and Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Ki67/MKI67 Antibody (SP6) - Unpurified

• antigen identified by monoclonal Ki-67
• Antigen Ki67
• antigen KI-67
• Ki67
• Ki-67
• KIA
• Marker Of Proliferation Ki-67
• MIB-
• MIB-1
• MKI67
• PPP1R105
• Proliferation Marker Protein Ki-67
• proliferation-related Ki-67 antigen
• Protein Phosphatase 1
• Regulatory Subunit 105
• TSG126

Background

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ki67/MKI67 Antibody (NB600-1252)(102)

Publications using NB600-1252

• Jobu Y, Nishigawa M, Furihata K et Al. Inhibitory effects of the combination of rapamycin with gemcitabine plus paclitaxel on the growth of pancreatic cancer tumors Hum Cell 2025-01-11 [PMID: 39794664]
• Gambarotto L, Russo L, Bresolin S et Al. Schwann Cell-Specific Ablation of Beclin 1 Impairs Myelination and Leads to Motor and Sensory Neuropathy in Mice Adv Sci (Weinh) 2024-12-16 [PMID: 39680476]
• Takahashi J, Sugihara HY, Kato S et Al. Controlled aggregative assembly to form self-organizing macroscopic human intestine from induced pluripotent stem cells Cell Rep Methods 2024-12-17 [PMID: 39662475]
• Tey SR, Anderson RS, Yu CH et al. Cellular and transcriptomic changes by the supplementation of aged rat serum in human pluripotent stem cell-derived myogenic progenitors Frontiers in Cell and Developmental Biology 2024-10-15 [PMID: 39474351]
• Hüser, L;Chhabra, Y;Gololobova, O;Wang, V;Liu, G;Dixit, A;Rocha, MR;Harper, EI;Fane, ME;Marino-Bravante, GE;Zabransky, DJ;Cai, KQ;Utikal, J;Slusher, BS;Walston, J;Lipson, EJ;Witwer, KW;Weeraratna, AT; Aged fibroblast-derived extracellular vesicles promote angiogenesis in melanoma Cell reports 2024-09-09 [PMID: 39255061]
• Chhabra Y, Fane ME, Pramod S, Hüser L et Al. Sex-dependent effects in the aged melanoma tumor microenvironment influence invasion and resistance to targeted therapy Cell 2024-09-07 [PMID: 39243764]
• Oami T, Abtahi S, Shimazui T et al. Claudin-2 upregulation enhances intestinal permeability, immune activation, dysbiosis, and mortality in sepsis. Proceedings of the National Academy of Sciences of the United States of America 2024-02-29 [PMID: 38412124]
• Leone MA, Gelati M, Profico DC et al. Phase I clinical trial of intracerebroventricular transplantation of allogeneic neural stem cells in people with progressive multiple sclerosis Cell stem cell 2023-11-21 [PMID: 38016468]
• Tateishi K, Miyake Y, Nakamura T et al. Genetic alterations that deregulate RB and PDGFRA signaling pathways drive tumor progression in IDH2-mutant astrocytoma Acta neuropathologica communications 2023-11-27 [PMID: 38012788] (IHC-P, Mouse)
• Hwang J, Cho Y, Ryu J et al. Ulipristal acetate, a selective progesterone receptor modulator, induces cell death via inhibition of STAT3/CCL2 signaling pathway in uterine sarcoma Biomedicine & Pharmacotherapy 2023-12-01 [PMID: 37924789] (IHC-P, Human)
• Tsubosaka A, Komura D, Kakiuchi M et al. Stomach encyclopedia: Combined single-cell and spatial transcriptomics reveal cell diversity and homeostatic regulation of human stomach Cell reports 2023-10-31 [PMID: 37819756] (Mouse)
• Bernabé-Rubio M, Ali S, Bhosale PG et al. Myc-dependent dedifferentiation of Gata6+ epidermal cells resembles reversal of terminal differentiation Nature cell biology 2023-09-21 [PMID: 37735598] (IHC-Fr, Mouse)
  
  Details:
  IHC-Fr: 1:50
• Ono N, Fujita T, Miki M et al. Interleukin-19 Gene-Deficient Mice Promote Liver Fibrosis via Enhanced TGF-? Signaling, and the Interleukin-19-CCL2 Axis Is Important in the Direction of Liver Fibrosis Biomedicines 2023-07-22 [PMID: 37509702] (IHC-Fr, Mouse)
  
  Details:
  1:500 dilution
• Goto T, Ueha R, Sato T, Yamasoba T. Effects of early local administration of high-dose bFGF on a recurrent laryngeal nerve injury model Journal of Otolaryngology - Head & Neck Surgery 2023-07-24 [PMID: 37488610]
• Chang CS, Ryu JY, Choi JK et al. Anti-cancer effect of fenbendazole-incorporated PLGA nanoparticles in ovarian cancer Journal of Gynecologic Oncology 2023-04-24 [PMID: 37170725]
• vorgelegt v, Wen W Expression and function of blood receptors ROR?, REVERBA and BMAL1 in human colorectal cancer Thesis 2022-01-01
• Amin A, Hairulhisyam N, Aqilah R et al. Impact of Gut Recolonization on Liver Regeneration: Hepatic Matrisome Gene Expression after Partial Hepatectomy in Mice International Journal of Molecular Sciences 2023-06-28 [PMID: 37445951] (Immunohistochemistry, Mouse)
• Shiota Sato Y, Elbadawy M, Suzuki K et al. Derivation of a new model of lung adenocarcinoma using canine lung cancer organoids for translational research in pulmonary medicine Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-07-03 [PMID: 37413906] (IHC-P, Canine)
• Imafuku K, Iwata H, Natsuga K et al. Zonula occludens-1 distribution and barrier functions are affected by epithelial proliferation and turnover rates Cell proliferation 2023-03-14 [PMID: 36919255] (IHC-Fr, Human)
  
  Details:
  Dilution used in IHC-Fr 1:200
• Marchini A, Ciulla MG, Antonioli B et al. Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels Frontiers in bioengineering and biotechnology 2023-02-23 [PMID: 36911193] (IHC-Fr, Human)
• Sun J, Belke D, Gui Y et al. Polo-like kinase 4 inhibitor CFI-400945 inhibits carotid arterial neointima formation but increases atherosclerosis Cell death discovery 2023-02-07 [PMID: 36750553] (ICC/IF, Mouse)
• Takahashi J, Mizutani T, Sugihara H et al. Suspension culture in a rotating bioreactor for efficient generation of human intestinal organoids Cell reports methods 2022-11-21 [PMID: 36452871] (IF/IHC, Human)
• Profico DC, Gelati M, Ferrari D et al. Human Neural Stem Cell-Based Drug Product: Clinical and Nonclinical Characterization International journal of molecular sciences 2022-11-03 [PMID: 36362211] (IHC-Fr, Human)
  
  Details:
  Dilution used in IHC-Fr 1:500
• van Schaik T, Manzo SG, Vouzas AE, Liu NQ et Al. Dynamic chromosomal interactions and control of heterochromatin positioning by Ki-67 EMBO Rep 2022-10-17 [PMID: 36245428]
• Shahzad T, Chao CM, Hadzic S et al. TRAIL protects the immature lung from hyperoxic injury Cell death & disease 2022-07-15 [PMID: 35840556] (ICC, Mouse)
• Ueha R, Ito T, Furukawa R et al. Oral SARS-CoV-2 Inoculation Causes Nasal Viral Infection Leading to Olfactory Bulb Infection: An Experimental Study Frontiers in Cellular and Infection Microbiology 2022-06-13 [PMID: 35770069]
• Noh JJ, Cho YJ, Ryu JY et al. Anti-cancer activity of the combination of cabozantinib and temozolomide in uterine sarcoma Clinical cancer research : an official journal of the American Association for Cancer Research 2022-06-21 [PMID: 35727598] (IHC-P, Human)
• ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna Stromal changes in the aged lung induce an emergence from melanoma dormancy Nature, 2022-06-01;606(7913):396-405. 2022-06-01 [PMID: 35650435]
• Lee AR, Woo JS, Lee SY et al. Mitochondrial Transplantation Ameliorates the Development and Progression of Osteoarthritis Immune Network 2022-01-21 [PMID: 35573148]
• Ando T, Tai-Nagara I, Sugiura Y et al. Tumor-specific inter-endothelial adhesion mediated by FLRT2 facilitates cancer aggressiveness The Journal of clinical investigation 2022-02-01 [PMID: 35104247] (IHC-WhMt, Mouse)
• Gremlich S, Cremona TP, Yao E et al. Tenascin-C: Friend or Foe in Lung Aging? Frontiers in Physiology 2021-10-27 [PMID: 34777012]
• Dufresne S, Richard C, Dieumegard A et al. Voluntary Wheel Running Does Not Enhance Radiotherapy Efficiency in a Preclinical Model of Prostate Cancer: The Importance of Physical Activity Modalities? Cancers (Basel) 2021-10-28 [PMID: 34771565]
• De Valliere C, Babler K, Busenhart P Et Al. A Novel OGR1 (GPR68) Inhibitor Attenuates Inflammation in Murine Models of Colitis Inflamm Intest Dis 2021-11-01 [PMID: 34722644]
• Kimishima Y, Misaka T, Yokokawa T Et al. Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils Nature communications 2021-10-26 [PMID: 34702814] (ICC/IF, Mouse)
• Mori E, Ueha R, Kondo K et al. Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection Frontiers in Neuroscience 2021-07-20 [PMID: 34354563]
• Garbati P, Barbieri R, Calderoni M et al. Efficacy of a Three Drug-Based Therapy for Neuroblastoma in Mice International Journal of Molecular Sciences 2021-06-23 [PMID: 34201814]
• Young LV, Morrison W, Campbell C et al. Loss of dystrophin expression in skeletal muscle is associated with senescence of macrophages and endothelial cells American journal of physiology. Cell physiology 2021-05-12 [PMID: 33979211]
• MOROKI T, MATSUO S, HATAKEYAMA H et al. Databases for technical aspects of immunohistochemistry-2021 update Journal of Toxicologic Pathology 2021-02-24 [PMID: 33976473]
• Hwang JR, Kim WY, Cho YJ et al. Chloroquine reverses chemoresistance via upregulation of p21WAF1/CIP1 and autophagy inhibition in ovarian cancer Cell death & disease 2020-12-04 [PMID: 33277461] (IF/IHC, Mouse)
• Douglass SM, Fane ME, Sanseviero E et al. Myeloid-Derived Suppressor Cells Are a Major Source of Wnt5A in the Melanoma Microenvironment and Depend on Wnt5A for Full Suppressive Activity Cancer Research 2021-02-01 [PMID: 33262126]
• Brizzolara A, Garbati P, Vella S et al. Co-Administration of Fendiline Hydrochloride Enhances Chemotherapeutic Efficacy of Cisplatin in Neuroblastoma Treatment Molecules 2020-11-10 [PMID: 33182713] (IF/IHC, Mouse)
• Garbati P, Barbieri R, Cangelosi D et al. MCM2 and Carbonic Anhydrase 9 Are Novel Potential Targets for Neuroblastoma Pharmacological Treatment Biomedicines 2020-11-03 [PMID: 33153038]
• Singhal NS, Bai M, Lee EM et al. Cytoprotection by a naturally occurring variant of ATP5G1 in Arctic ground squirrels Elife 2020-10-14 [PMID: 33050999]
• Hvid H, Skydsgaard M, Jensen NK et al. Artificial Intelligence-Based Quantification of Epithelial Proliferation in Mammary Glands of Rats and Oviducts of Gottingen Minipigs Toxicol Pathol 2020-08-25 [PMID: 32840183]
• Dani C, GonCalves LK, ProenCa IT et al. Effects of Maternal Grape Juice Intake on Unfolded Protein Response in the Mammary Glands of Offspring of High Fat Diet Fed Rat Dams Nutrients 2020-07-28 [PMID: 32731460] (IF/IHC, Rat)
• Tibaldi E, Gnudi F, Panzacchi S et al. Identification of aspartame-induced haematopoietic and lymphoid tumours in rats after lifetime treatment Acta Histochemica 2020-01-01 [PMID: 32622430] (IF/IHC, Rat)
• Nagai T, Ishida C, Nakamura T et al. Focal Adhesion Kinase-Mediated Sequences, Including Cell Adhesion, Inflammatory Response, and Fibrosis, as a Therapeutic Target in Endometriosis Reprod Sci 2020-04-23 [PMID: 32329031]
• Lee J, Kwon M, Oh N et al. Cell-Penetrating Peptides Enhance the Activity of Human Fibroblast Growth Factor 2 by Prolonging the Retention Time: A New Vision for Drug-Delivery Systems Int J Mol Sci 2020-04-15 [PMID: 32284513]
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• Gattlen C, Deftu AF, Tonello R et al. The inhibition of Kir2.1 potassium channels depolarizes spinal microglial cells, reduces their proliferation, and attenuates neuropathic pain Glia 2020-03-27 [PMID: 32220118] (IF/IHC, IHC-FrFl, Mouse)
• Paik Es, Kim Th, Cho Yj et Al. Preclinical assessment of the VEGFR inhibitor axitinib as a therapeutic agent for epithelial ovarian cancer Sci Rep 2020-03-17 [PMID: 32184452] (IF/IHC, Human)
• Ueha R, Ueha S, Kondo K et al. Effects of Cigarette Smoke on the Nasal Respiratory and Olfactory Mucosa in Allergic Rhinitis Mice Front. Neurosci. 2020-02-18 [PMID: 32132898] (IF/IHC, Mouse)
• Dufresne S, Gueritat J, Chiavassa S et al. Exercise training improves radiotherapy efficiency in a murine model of prostate cancer FASEB J. 2020-02-11 [PMID: 32043634] (IHC-P, Mouse)
• Minakawa A, Fukuda A, Sato Y Podocyte hypertrophic stress and detachment precedes hyperglycemia or albuminuria in a rat model of obesity and type2 diabetes-associated nephropathy Sci Rep 2019-12-06 [PMID: 31811176] (ICC/IF, Rat)
• Kim J, Cho YJ, Ryu JY et al. CDK7 is a reliable prognostic factor and novel therapeutic target in epithelial ovarian cancer Gynecol. Oncol. 2019-11-24 [PMID: 31776040]
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• Nama S, Muhuri M, Di Pascale F et al. MicroRNA-138 is a Prognostic Biomarker for Triple-Negative Breast Cancer and Promotes Tumorigenesis via TUSC2 repression Sci Rep 2019-09-03 [PMID: 31481748] (KD, IF/IHC, Human)
  
  Details:
  The Ki-67 antibody was used for genetic strategies validation and was used to validate MiR-138 knockdown, as Ki-67 is a marker for proliferation.
• Hayashi M, Madokoro H, Yamada K et al. Novel Antibody-Drug Conjugate with Anti-CD26 Humanized Monoclonal Antibody and Transcription Factor IIH (TFIIH) Inhibitor, Triptolide, Inhibits Tumor Growth via Impairing mRNA Synthesis Cancers (Basel) 2019-08-08 [PMID: 31398954] (In Vivo, Mouse)
• Norgaard SA, Briand F, Sand FW et al. Nephropathy in diabetic db/db mice is accelerated by high protein diet and improved by the SGLT2 inhibitor dapagliflozin Eur. J. Pharmacol. 2019-07-13 [PMID: 31310751] (IF/IHC, Mouse)
• Winham CL, Le T, Jellison ER et al. gamma-Synuclein Induces Human Cortical Astrocyte Proliferation and Subsequent BDNF Expression and Release Neuroscience 2019-05-10 [PMID: 31078687] (WB, ICC/IF, Human)
• Wada H, Ikoma K, Oka Y et al. Human 3D cellular model of hypoxic brain injury of prematurity Nat. Med. 2019-05-01 [PMID: 31061540] (IHC-Fr, Human)
• Kalamakis G, Brune D, Ravichandran S et al. Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain Cell 2019-02-22 [PMID: 30827680] (ICC/IF, Mouse)
• Goto T, Ueha R, Sato T et al. Single, high-dose local injection of bFGF improves thyroarytenoid muscle atrophy after paralysis Laryngoscope 2019-02-27 [PMID: 30810241] (IF/IHC, Rat)
• Ueha R, Kondo K, Ueha S, Yamasoba T. Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium Front Aging Neurosci 2018-11-20 [PMID: 30515092] (IF/IHC, Mouse)
• Kato N, Iwase A, Ishida C et al. Upregulation of Fibroblast Growth Factors Caused by Heart and Neural Crest Derivatives Expressed 2 Suppression in Endometriotic Cells: A Possible Therapeutic Target in Endometriosis. Reprod Sci. 2018-10-01 [PMID: 30270745] (IF/IHC, Mouse)
• Ueha R, Ueha S, Kondo K et al. Cigarette Smoke-Induced Cell Death Causes Persistent Olfactory Dysfunction in Aged Mice Front Aging Neurosci 2018-06-13 [PMID: 29950987] (IHC-P, Mouse)
• Burks J, Nadella S, Mahmud A et al. Cholecystokinin Receptor-Targeted Polyplex Nanoparticle Inhibits Growth and Metastasis of Pancreatic Cancer Cell Mol Gastroenterol Hepatol 2018-03-07 [PMID: 29928669] (IHC-Fr, Human)
• Ueha R, Shichino S, Ueha S et al. Reduction of Proliferating Olfactory Cells and Low Expression of Extracellular Matrix Genes Are Hallmarks of the Aged Olfactory Mucosa. Front Aging Neurosci 2018-03-27 [PMID: 29636678] (Mouse)
• Pucci Molineris M, Gonzalez Polo V, Perez F et al. Paneth and intestinal stem cells preserve their functional integrity during worsening of acute cellular rejection in small bowel transplantation Am. J. Transplant. [PMID: 29139621] (IF/IHC, Mouse)
• Nishizono R, Kikuchi M, Wang SQ et al. FSGS as an Adaptive Response to Growth-Induced Podocyte Stress. J. Am. Soc. Nephrol. 2017-10-01 [PMID: 28720684] (ICC/IF, Rat)
• Penna I, Gigoni A, Costa D et al. The inhibition of 45A ncRNA expression reduces tumor formation, affecting tumor nodules compactness and metastatic potential in neuroblastoma cells. Oncotarget. 2017-01-31 [PMID: 28029658] (IF/IHC, Mouse)
• Sumis A, Cook K, Andrade FO et al. Social isolation induces autophagy in the mouse mammary gland: link to increased mammary cancer risk. Endocr. Relat. Cancer. 2016-08-22 [PMID: 27550962] (IF/IHC, Mouse)
• Kalliokoski S, Piqueras VO, Frias R et al. Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice. Amino Acids. 2016-08-09 [PMID: 27503559] (IF/IHC, Mouse)
• Rath SK, Deb M, Sengupta D et al. Silencing of ZRF1 impedes survival of estrogen receptor positive MCF-7 cells and potentiates the effect of curcumin. Tumour Biol 2016-09-01 [PMID: 27350366] (IF/IHC, Human)
• Ueha R, Ueha S, Sakamoto T et al. Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice. Neurotox Res 2016-08-01 [PMID: 27003941]
• Migita H, Yoshitake S, Tange Y et al. Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular Regeneration After Renal Ischemia/Reperfusion Injury in Rats. Nephrourol Mon 2016-01-01 [PMID: 26981502]
• Ueha R, Ueha S, Kondo K et al. Damage to Olfactory Progenitor Cells Is Involved in Cigarette Smoke-Induced Olfactory Dysfunction in Mice. Am J Pathol 2016-03-01 [PMID: 26806086]
• Falkenberg N, Hofig I, Rosemann M et al. Three-dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer. Cancer Med. 2016-01-14 [PMID: 26763588] (IHC-P, Human)
• Du Z, Bondarenko O, Wang D et al. Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression. J. Cell. Physiol. 2015-10-30 [PMID: 26516696] (IHC-P, Rat)
• Vesci L, Carollo V, Roscilli G et al. [Corrigendum] Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance. Oncol Rep 2016-01-01 [PMID: 26499228]
• Jang H, Padanilam B Simultaneous deletion of Bax and Bak is required to prevent apoptosis and interstitial fibrosis in obstructive nephropathy. Am J Physiol Renal Physiol 2015-09-15 [PMID: 26180237] (IF/IHC, Mouse)
• Vesci L, Carollo V, Roscilli G et al. Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance Oncol. Rep. 2015-06-24 [PMID: 26133490] (IHC-P, Human)
• Tukler Henriksson J, Coursey TG, Corry DB et al. IL-13 Stimulates Proliferation and Expression of Mucin and Immunomodulatory Genes in Cultured Conjunctival Goblet Cells. Invest Ophthalmol Vis Sci 2015-07-01 [PMID: 26132778] (ICC/IF, Mouse)
• Kim LA, Wong LL, Amarnani DS et al. Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy Mol. Vis. 2015-06-29 [PMID: 26120272] (IHC-P, Human)
• Cage TA, Chanthery Y, Chesler L. Downregulation of MYCN through PI3K inhibition in mouse models of pediatric neural cancer Front. Oncol. 2015-04-27 [PMID: 26029667] (IHC-P, Human)
• Sugiyama Y, Yamamoto K, Honda T et al. Impact of Elobixibat on Liver Tumors, Microbiome and Bile Acid Levels in a Mouse Model of Nonalcoholic Steatohepatitis Research Square 2023-05-23 (IHC, Mouse)
• Schevzov G, Kee AJ, Wang B et al. Regulation of cell proliferation by ERK and signal-dependent nuclear translocation of ERK is dependent on Tm5NM1-containing actin filaments. Mol. Biol. Cell. 2015-05-13 [PMID: 25971798] (IHC-P, Mouse)
• Braks Joanna a M, Spiegelberg Linda, Koljenovic Senada et al. Optical Imaging of Tumor Response to Hyperbaric Oxygen Treatment and Irradiation in an Orthotopic Mouse Model of Head and Neck Squamous Cell Carcinoma. Molecular Imaging and Biology : Mib : the Official Publication of the Academy of Molecular Imaging 2015-01-01 [PMID: 25724406] (IF/IHC, Mouse)
• Kalliokoski S, Caja S, Frias R et al. Injection of celiac disease patient sera or immunoglobulins to mice reproduces a condition mimicking early developing celiac disease. J Mol. Med. 2014-09-12 [PMID: 25209899]
• Papazoglou IK, Jean A, Gertler A et al. Hippocampal GSK3B as a Molecular Link Between Obesity and Depression Mol. Neurobiol. 2014-08-29 [PMID: 25169083]
• Orticello M, Fiore M, Totta P et al. N-terminus-modified Hec1 suppresses tumour growth by interfering with kinetochore-microtubule dynamics. Oncogene. 2014-08-18 [PMID: 25132262] (IHC-P, Mouse)
• Pusic AD, Kraig RP. Youth and environmental enrichment generate serum exosomes containing miR-219 that promote CNS myelination. Glia 2014-02-01 [PMID: 24339157] (IHC-Fr, Rat)
• Vulpio C, Bossola M, Di Stasio E et al. Histology and immunohistochemistry of the parathyroid glands in renal secondary hyperparathyroidism refractory to vitamin D or cinacalcet therapy. Eur J Endocrinol. 2013-04-29 [PMID: 23520248] (IHC-P, Human)
• Yee KK, Li Y, Redding KM, Iwatsuki K et al. Lgr5-EGFP Marks Taste Bud Stem/Progenitor Cells in Posterior Tongue. Stem Cells 2013-02-04 [PMID: 23377989]
• Hua K, Schindler MK, McQuail JA et al. Regionally distinct responses of microglia and glial progenitor cells to whole brain irradiation in adult and aging rats. PLoS One. 2012-12-26 [PMID: 23300752] (IHC-Fr, Rat)
• Kaenel P, Antonijevic M, Richter S et al. Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway. Int J Oncol. 2012-02-01 [PMID: 22020958] (IHC-P, Mouse)
• Pageau SC, Sazonova OV, Wong JY et al. The effect of stromal components on the modulation of the phenotype of human bronchial epithelial cells in 3D culture. Biomaterials. 2011-10-01 [PMID: 21724251] (IHC-P, Human)
• F J Swartling, M R Grimmer, C S Hackett et al. Pleiotropic role for MYCN in medulloblastoma. Genes & Dev. 24 (10): 1059-1072. 2010-01-01 [PMID: 20478998]
• Zambon AC. Use of the Ki67 promoter to label cell cycle entry in living cells. Cytometry A. 2010-06-01 [PMID: 20235278] (ICC/IF, Human)
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• Koya V, Lu S, Sun Y-P et al. Reversal of Streptozotocin-Induced Diabetes in Mice by Cellular Transduction With Recombinant Pancreatic Transcription Factor Pancreatic Duodenal Homeobox-1: A Novel Protein Transduction Domain-Based Therapy. Diabetes;57(3):757-69. 2008-03-01 [PMID: 18086901] (IHC-P)
• Frirdich E, Lindner B, Holst O, Whitfield C. Overexpression of the waaZ gene leads to modification of the structure of the inner core region of Escherichia coli lipopolysaccharide, truncation of the outer core, and reduction of the amount of O polysaccharide on the cell surface. J Bacteriol 2003-03-01 [PMID: 12591884]

Reviews for Ki67/MKI67 Antibody (NB600-1252) (8)

Average Rating: 4.5

(Based on 8 reviews)

We have 8 reviews tested in 5 species: Human, Mouse, Rat, Dog, Horse.

reviewed by: Verified Customer IHC-P Human 04/15/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Tonsil epithelium
• Species: Human
• Lot: T823

Comments

• Comments: Tissue sections were subject to 20min heat induced epitope retrieval in a vegetable steamer using Dako S1699. Secondary was horse anti rabbit HRP polymer.

reviewed by: Verified Customer IHC-P Horse 04/15/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Intestine and spleen
• Species: Horse
• Lot: T823

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Tissue sections were subject to 20min heat induced epitope retrieval in a vegetable steamer. Secondary was horse anti rabbit HRP polymer.

reviewed by: Verified Customer IHC-P Dog 04/15/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Cecum and skin
• Species: Dog
• Lot: T823

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Tissue sections were subject to 20min heat induced epitope retrieval in a vegetable steamer. Secondary was horse anti rabbit HRP polymer.

reviewed by: Kevin Thorburn IHC-P Mouse 04/06/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: FFPE
• Species: Mouse
• Lot: T823

Comments

• Comments: Heat induced epitope retrieval carried out using a vegetable steamer and citrate buffer for 20m. Primary diluted 1 to 100 Antibody Diluent and left on sections for 1h at room temperature. Primary antibody was detected with Horse Anti-Rabbit HRP left on sections for 30m at room temperature. DAB used as a chromogen with hematoxylin counterstain

reviewed by: Bhavin Shah IF Mouse 12/23/2021

Summary

• Application: Immunofluorescence
• Sample Tested: embroynic mouse brain E18 tissue
• Species: Mouse
• Lot: N671

Comments

• Comments: Antigen retrieval using 10mM sodium citrate may enhance the signal.
  The staining may not work good in adult tissues using the vibratome tissues. Standardisation for adult brain tissues would be required.

reviewed by: Verified Customer IF-paraffin Rat 04/25/2019

Summary

• Application: Other
• Sample Tested: Kidney tissue,Kidney
• Species: Rat
• Lot: G068

reviewed by: Verified Customer IHC-P Rat 02/17/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Spleen tissue
• Species: Rat
• Lot: E213

Comments

• Comments: Paraffin-embedded alcohol fixed rat spleen tissue. Antigen retrieval pH 9. Ki67 dilution 1:100 incubation ON 4°C.

reviewed by: Feng Li ICC Mouse 05/30/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: thyroid tumor
• Species: Mouse
• Lot: G746

FAQs for Ki67/MKI67 Antibody (NB600-1252). (Showing 1 - 2 of 2 FAQs).

1. I have bought via kristalindo biolab (Indonesia) primary antibody ki-67 (catalog # NB600-1252). Do you have a positive control for that ? My sample is breast cancer from rat.
   • In our lab, we have tested this product in tonsil tissues and we recommend either using a tonsil tissue from rat or human as a positive control to determine that the staining you see in your rat breast cancer tissue is specific. We do have a tonsil slide that you might find useful (catalog # S152W0).
2. I am using the Ki-67 antibody (NB600-1252) and I need some information about the protein concentration.
   • Please note that this product is a "Tissue Culture Supernatant" production and the exact concentration of the antibody is not quantifiable in it. Technically speaking, the tissue culture supernatants can have the IgG contents from 1 - 3 mg/ml.

Bioinformatics

• Gene Symbol: MKI67
• Entrez:
  • 4288(Human)
  • 291234(Rat)