Ki67/MKI67 Antibody (SP6) - Unpurified

Images

 
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - T47D cells form functional 3D spheroids in a scaffold-free system. Sections of 3D microtissues grown for 12 days were ...read more
Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - PW-12 is efficacious in an orthotopic flank tumor model of MYCN-amplified medulloblastoma. Mice were implanted with 6 x 106 SmoWT ...read more
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody.
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Ki-67/MKI67 Antibody (SP6) [NB600-1252] - Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody.
Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Paraffin-embedded alcohol fixed rat spleen tissue (20x). Antigen retrieval pH 9. Ki67 dilution 1:100 incubation ON 4C. This image was ...read more
Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - PW-12 is efficacious in a GEM model of MYCN-amplified neuroblastoma. Tumor-bearing TH-MYCN mice were treated for 14 days with either 25 mg/kg ...read more
Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Ki-67/MKI67 Antibody (SP6) [NB600-1252] - Ki67 staining in mouse thyroid tissue at pre-tumor stage (green). Dilution is 1:100. This image was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, In vivo, KD
Clone
SP6
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Unpurified

Order Details

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Catalog# & Formulation Size Price

Ki67/MKI67 Antibody (SP6) - Unpurified Summary

Immunogen
The immunogen for this unpurified KI67/MKI67 Antibody (SP6) was made using a synthetic peptide from the C-Terminus of Human KI67/MKI67.
Epitope
C-terminus
Localization
Nuclear
Marker
Proliferation Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MKI67
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:25-1:50
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:50
  • In vivo assay
  • Knockdown Validated
  • Western Blot
Application Notes
IHC-P: recommended incubation time of 30-60 min at RT. Ki67/MKI67 Antibody (SP6) was used for ICC/IF (PMID: 20235278) and IHC-Fr reported in scientific literature (PMID: 23300752). Use in Western blot reported in scientific literature (PMID: 31078687). Use In vivo reported in scientific literature (PMID:31398954).
Theoretical MW
359 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Tonsil Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB600-1252 in the following applications:

Publications
Read Publications using
NB600-1252 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32731460). Ki67/MKI67 (SP6). antibody reacted with Mouse (PMID: 22020958). and Rat (PMID: 30810241). .

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Tissue culture supernatant
Preservative
0.05% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Ki67/MKI67 Antibody (SP6) - Unpurified

  • antigen identified by monoclonal Ki-67
  • Antigen Ki67
  • antigen KI-67
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-
  • MIB-1
  • MKI67
  • PPP1R105
  • Proliferation Marker Protein Ki-67
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ki67/MKI67 Antibody (NB600-1252)(63)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, In vivo, KD, WB.


Filter By Application
ICC/IF
(11)
IHC
(20)
IHC-Fr
(4)
IHC-FrFl
(1)
IHC-P
(13)
In vivo
(1)
KD
(1)
WB
(1)
All Applications
Filter By Species
Human
(15)
Mouse
(26)
Rat
(8)
All Species
Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using NB600-1252 Applications Species
Kimishima Y, Misaka T, Yokokawa T Et al. Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils Nature communications Oct 26 2021 [PMID: 34702814] (ICC/IF, Mouse) ICC/IF Mouse
Young LV, Morrison W, Campbell C et al. Loss of dystrophin expression in skeletal muscle is associated with senescence of macrophages and endothelial cells American journal of physiology. Cell physiology May 12 2021 [PMID: 33979211]
Hwang JR, Kim WY, Cho YJ et al. Chloroquine reverses chemoresistance via upregulation of p21WAF1/CIP1 and autophagy inhibition in ovarian cancer Cell death & disease Dec 4 2020 [PMID: 33277461] (IHC, Mouse) IHC Mouse
Brizzolara A, Garbati P, Vella S et al. Co-Administration of Fendiline Hydrochloride Enhances Chemotherapeutic Efficacy of Cisplatin in Neuroblastoma Treatment Molecules Nov 10 2020 [PMID: 33182713] (IHC, Mouse) IHC Mouse
Hvid H, Skydsgaard M, Jensen NK et al. Artificial Intelligence-Based Quantification of Epithelial Proliferation in Mammary Glands of Rats and Oviducts of Gottingen Minipigs Toxicol Pathol Aug 25 2020 [PMID: 32840183]
Tibaldi E, Gnudi F, Panzacchi S et al. Identification of aspartame-induced haematopoietic and lymphoid tumours in rats after lifetime treatment Acta Histochemica Jan 1 2020 [PMID: 32622430] (IHC, Rat) IHC Rat
Nagai T, Ishida C, Nakamura T et al. Focal Adhesion Kinase-Mediated Sequences, Including Cell Adhesion, Inflammatory Response, and Fibrosis, as a Therapeutic Target in Endometriosis Reprod Sci Apr 23 2020 [PMID: 32329031]
Gattlen C, Deftu AF, Tonello R et al. The inhibition of Kir2.1 potassium channels depolarizes spinal microglial cells, reduces their proliferation, and attenuates neuropathic pain Glia Mar 27 2020 [PMID: 32220118] (IHC, IHC-FrFl, Mouse) IHC, IHC-FrFl Mouse
Paik Es, Kim Th, Cho Yj et Al. Preclinical assessment of the VEGFR inhibitor axitinib as a therapeutic agent for epithelial ovarian cancer Sci Rep Mar 17 2020 [PMID: 32184452] (IHC, Human) IHC Human
Ueha R, Ueha S, Kondo K et al. Effects of Cigarette Smoke on the Nasal Respiratory and Olfactory Mucosa in Allergic Rhinitis Mice Front. Neurosci. Feb 18 2020 [PMID: 32132898] (IHC, Mouse) IHC Mouse
Show All 63 Publications.

Reviews for Ki67/MKI67 Antibody (NB600-1252) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, Rat.

Reviews using NB600-1252:
Filter by Applications
IF
(1)
IHC-P
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Ki67/MKI67 NB600-1252
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4
reviewed by:
Bhavin Shah
IF Mouse 12/23/2021
View

Summary

ApplicationImmunofluorescence
Sample Testedembroynic mouse brain E18 tissue
SpeciesMouse
LotN671

Comments

CommentsAntigen retrieval using 10mM sodium citrate may enhance the signal.
The staining may not work good in adult tissues using the vibratome tissues. Standardisation for adult brain tissues would be required.
 Ki67/MKI67 NB600-1252
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5
reviewed by:
Verified Customer
Rat 04/25/2019
View

Summary

Sample TestedKidney,Kidney tissue
SpeciesRat
LotG068
Immunohistochemistry-Paraffin Ki67/MKI67 NB600-1252
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5
reviewed by:
Verified Customer
IHC-P Rat 02/17/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSpleen tissue
SpeciesRat
LotE213

Comments

CommentsParaffin-embedded alcohol fixed rat spleen tissue. Antigen retrieval pH 9. Ki67 dilution 1:100 incubation ON 4°C.
Immunocytochemistry Ki67/MKI67 NB600-1252
Enlarge
5
reviewed by:
Feng Li
ICC Mouse 05/30/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedthyroid tumor
SpeciesMouse
LotG746

Product General Protocols

View specific protocols for Ki67/MKI67 Antibody (NB600-1252): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ki67/MKI67 Antibody (NB600-1252). (Showing 1 - 2 of 2 FAQs).

  1. I have bought via kristalindo biolab (Indonesia) primary antibody ki-67 (catalog # NB600-1252). Do you have a positive control for that ? My sample is breast cancer from rat.
    • In our lab, we have tested this product in tonsil tissues and we recommend either using a tonsil tissue from rat or human as a positive control to determine that the staining you see in your rat breast cancer tissue is specific. We do have a tonsil slide that you might find useful (catalog # S152W0).
  2. I am using the Ki-67 antibody (NB600-1252) and I need some information about the protein concentration.
    • Please note that this product is a "Tissue Culture Supernatant" production and the exact concentration of the antibody is not quantifiable in it. Technically speaking, the tissue culture supernatants can have the IgG contents from 1 - 3 mg/ml.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Ki67/MKI67.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Ki67 - an established marker for labelling proliferating cells
Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v...  Read full blog post.

Ki67 - A Crucial Cellular Proliferation Marker
The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line....  Read full blog post.

The Ki67 Antibody in Cell Marker Studies
The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is ...  Read full blog post.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Bhavin Shah
12/23/2021
Application: IF
Species: Mouse

Verified Customer
04/25/2019
Application:
Species: Rat

Verified Customer
02/17/2018
Application: IHC-P
Species: Rat

Bioinformatics

Gene Symbol MKI67
Entrez