KGF/FGF-7 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

KGF/FGF-7 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human KGF/FGF-7 (R&D Systems, Catalog # 251-KG)
  Cys32-Thr194
  Accession # Q6FGV5
• Specificity: Detects human KGF/FGF-7 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, and rhFGF-6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGF7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human KGF/FGF-7 Antibody (Catalog # MAB251)
  ELISA Detection: Human KGF/FGF-7 Biotinylated Antibody (Catalog # BAF251)
  Standard: Recombinant Human KGF/FGF-7 (Catalog # 251-KG)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KGF/FGF-7 Antibody [Biotin]

• FGF7
• FGF-7
• fibroblast growth factor 7HBGF-7
• HBGF7
• HBGF-7
• Heparin-binding growth factor 7
• keratinocyte growth factor
• KGF
• KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1 - 4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha . (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
8. Qiao, J. et al. (1999) Development 126:547.
9. Guo, L. et al. (1996) Genes Dev. 10:165.
10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
12. Belleudi, F. et al. (2007) Traffic 8:1854.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KGF/FGF-7 Antibody (BAF251)(1)

Publication using BAF251

• Putnins EE, Sanaie AR, Wu Q Induction of keratinocyte growth factor 1 Expression by lipopolysaccharide is regulated by CD-14 and toll-like receptors 2 and 4. Infect. Immun., 2002-12-01;70(12):6541-8. 2002-12-01 [PMID: 12438323] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: FGF7
• Uniprot:
  • Q6FGV5()