KGF/FGF-7 Antibody

KGF/FGF‑7 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human KGF/FGF‑7 (Catalog # 251-KG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human KGF/FGF‑7 (125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA). The ND50 is typically 6-12 µg/mL.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

KGF/FGF-7 Antibody Summary

• Immunogen: E. coli-derived recombinant human KGF/FGF‑7
  Cys32-Thr194
  Accession # Q6FGV5
• Specificity: Detects human KGF/FGF‑7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse FGF-7 is observed, less than 20% cross-reactivity with recombinant human (rh) FGF-9 is observed, and less than 10% cross‑reactivity with rhFGF-4 and rhFGF-9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGF7
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 6-12 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KGF/FGF-7 Antibody

• FGF7
• FGF-7
• fibroblast growth factor 7HBGF-7
• HBGF7
• HBGF-7
• Heparin-binding growth factor 7
• keratinocyte growth factor
• KGF
• KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1‑4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
8. Qiao, J. et al. (1999) Development 126:547.
9. Guo, L. et al. (1996) Genes Dev. 10:165.
10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
12. Belleudi, F. et al. (2007) Traffic 8:1854.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KGF/FGF-7 Antibody (AF-251-NA)(5)

Publications using AF-251-NA

• H Yabuki, S Wakao, Y Kushida, M Dezawa, Y Okada Human Multilineage-differentiating Stress-Enduring Cells Exert Pleiotropic Effects to Ameliorate Acute Lung Ischemia-Reperfusion Injury in a Rat Model Cell Transplant, 2018;0(0):9636897187616. 2018 [PMID: 29707971] (Western Blot, Rat)
• Nadworny PL, Wang J, Tredget EE Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. J Inflamm (Lond), 2010;7(0):13. 2010 [PMID: 20170497] (IHC-P, Porcine)
• Skibinski G, Elborn JS, Ennis M Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76. 2007 [PMID: 17384084] (Neutralization, Human)
• Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2006;47(3):864-72. 2006 [PMID: 16505018] (Western Blot, Human)
• Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M Salivary leptin induces increased expression of growth factors in oral keratinocytes. J. Mol. Endocrinol., 2005;34(2):353-66. 2005 [PMID: 15821102] (Western Blot, Human)

Reviews for KGF/FGF-7 Antibody (AF-251-NA) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Anonymous WB Mouse 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24046361
• Species: Mouse

Blocking

• Blocking Details: See PMID 24046361

Details

• Detection Notes: See PMID 24046361

Comments

• Comments: Published: See PMID 24046361

reviewed by: Anonymous ELISA Human 01/07/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 23582717
• Species: Human

Blocking

• Blocking Details: See PMID 23582717

Details

• Detection Notes: See PMID 23582717

Comments

• Comments: Published: See PMID 23582717

Bioinformatics

• Gene Symbol: FGF7
• Uniprot:
  • Q6FGV5()