KGF/FGF-7 Antibody

Images

 
KGF/FGF‑7 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) at 10 ...read more
Recombinant Human KGF/FGF‑7 (Catalog # 251-KG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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KGF/FGF-7 Antibody Summary

Immunogen
E. coli-derived recombinant human KGF/FGF‑7
Cys32-Thr194
Accession # Q6FGV5
Specificity
Detects human KGF/FGF‑7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse FGF-7 is observed, less than 20% cross-reactivity with recombinant human (rh) FGF-9 is observed, and less than 10% cross‑reactivity with rhFGF-4 and rhFGF-9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FGF7
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 6-12 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF-251-NA in the following applications:

Publications
Read Publications using
AF-251-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KGF/FGF-7 Antibody

  • FGF7
  • FGF-7
  • fibroblast growth factor 7HBGF-7
  • HBGF7
  • HBGF-7
  • Heparin-binding growth factor 7
  • keratinocyte growth factor
  • KGF
  • KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1‑4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KGF/FGF-7 Antibody (AF-251-NA)(5)

We have publications tested in 3 confirmed species: Human, Rat, Porcine.

We have publications tested in 3 applications: IHC-P, Neutralization, Western Blot.


Filter By Application
IHC-P
(1)
Neutralization
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(3)
Rat
(1)
Porcine
(1)
All Species

Reviews for KGF/FGF-7 Antibody (AF-251-NA) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using AF-251-NA:
Filter by Applications
WB
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Mouse 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24046361
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24046361

Details

Detection NotesSee PMID 24046361

Comments

CommentsPublished: See PMID 24046361
  4
reviewed by:
Anonymous
ELISA Human 01/07/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID 23582717
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23582717

Details

Detection NotesSee PMID 23582717

Comments

CommentsPublished: See PMID 23582717

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF251

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Bioinformatics Tool for KGF/FGF-7 Antibody (AF-251-NA)

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
01/07/2015
Application: WB
Species: Mouse

 
Anonymous
01/07/2015
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol FGF7
Uniprot