KAT3B/p300 Antibody

Images

 
Western Blot: KAT3B/p300 Antibody [NB500-161] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 ...read more
Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Western Blot: KAT3B/p300 Antibody [NB500-161] - Analysis of HeLa lysates using NB500-161. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).
Western Blot: KAT3B/p300 Antibody [NB500-161] - Nuclear (NE) or cytosolic (S100) extract (10 mg) from HeLa cells. Antibody used at 20 ug/10 mg lysate.
Biological Strategies: Western Blot: KAT3B/p300 Antibody [NB500-161] - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified ...read more
KAT3B/p300 Antibody [NB500-161] - Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

KAT3B/p300 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EP300
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:500 to 1:2000
  • Immunohistochemistry-Paraffin 1:500 to 1:2000
  • Immunoprecipitation 1-4 ug/mg of lysate
Control
HeLa Nuclear Cell Lysate
HeLa Whole Cell Lysate
KAT3B/p300 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB500-161 in the following applications:

Publications
Read Publications using
NB500-161 in the following applications:

  • WB
    3 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:33106900).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KAT3B/p300 Antibody

  • E1A binding protein p300
  • E1A-associated protein p300
  • E1A-binding protein, 300kD
  • EC 2.3.1
  • EC 2.3.1.48
  • EP300
  • histone acetyltransferase p300
  • KAT3B
  • p300 HAT
  • p300
  • RSTS2

Background

p300 is highly homologous to CREB-binding protein (CBP). Like CBP, it is a conserved transcriptional coactivator and histone acetyltransferase (HAT) that links chromatin remodeling with transcription. It also plays a role in cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Rt
Applications: WB, ChIP, IHC, IHC-P, IP

Publications for KAT3B/p300 Antibody (NB500-161)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
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Filter By Species
Human
(1)
Rat
(1)
All Species

Review for KAT3B/p300 Antibody (NB500-161) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-161:
Filter by Applications
ChIP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation KAT3B/p300 NB500-161
Enlarge
5
reviewed by:
Anonymous
ChIP Human 10/07/2010
View

Summary

ApplicationChromatin Immunoprecipitation
Sample Testedhypoxic hela cell
SpeciesHuman

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 3 ug, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Details

Detection NotesDetection Method: Real-time PCR, ChIP Type: Cross-linking (X-ChIP)

Product General Protocols

View specific protocols for KAT3B/p300 Antibody (NB500-161): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for KAT3B/p300 Antibody (NB500-161) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for KAT3B/p300 Antibody (NB500-161)

Discover related pathways, diseases and genes to KAT3B/p300 Antibody (NB500-161). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on KAT3B/p300

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/07/2010
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol EP300
Entrez
Uniprot