JMJD2D Antibody [FITC]

• Reactivity: Hu, Pm
• Applications: WB, ChIP, ChIP, ICC/IF (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

JMJD2D Antibody [FITC] Summary

• Immunogen: Synthetic peptide made to a C-terminal portion of the human JMJD2D protein (within residues 460-520). [Swiss-Prot# Q6B0I6]
• Localization: Nucleus
• Predicted Species: Primate (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM4D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: monkey (92%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for JMJD2D Antibody [FITC]

• EC 1.14.11
• EC 1.14.11.-
• FLJ10251
• JHDM3D
• JmjC domain-containing histone demethylation protein 3D
• JMJD2D
• JMJD2Dlysine-specific demethylase 4D
• jumonji domain containing 2D
• Jumonji domain-containing protein 2D
• KDM4D
• lysine (K)-specific demethylase 4D
• MGC141909

Background

Jumonji lysine demethylases (JMJDs) are oxygenases catalyzing the demethylation of mono-, di-, or trimethylated lysines, and its members (JMJD2A, JMJD2B, JMJD2C, and JMJD2D/ KDM4D) are Fe(II)-/alpha-ketoglutarate-dependent enzymes harboring a JmjC catalytic domain. JMJD2 shows differential substrate specificities and unlike, JMJD2A, JMJD2B, and JMJD2C that efficiently demethylate H3K9me3 as well as H3K36me3, JMJD2D is specific for H3K9me3 site. These enzymes have been implicated in transcriptional regulation, cell-cycle progression, nuclear hormone signaling, embryonic stem cell self-renewal and development. Overexpression of several JMJD2 homologs has been linked to cancer e.g. JMJD2D, JMJD2A and JMJD2C are overexpressed in prostate cancer wherein they function as AR coactivators to upregulate AR target genes and JMJD2D has been shown to function as a pro-proliferative protein in colon cancer cells. JMJD2D has also been reported to interact with the ligand binding domain of AR and thereby activate AR gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDM4D