JAM-C Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of JAR human choriocarcinoma cell line and human placenta, loaded at 0.2 mg/mL. A specific band was detected for JAM‑C at approximately 53 and 54 kDa (as indicated) using ...read more
Western blot shows lysates of human placenta tissue, human brain tissue, JAR human choriocarcinoma cell line, and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of ...read more
Recombinant Human JAM-B/VE-JAM Fc Chimera (Catalog # 1074-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G‑102‑C), supports the adhesion of the J45.01 human acute ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

JAM-C Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM-C
Val32-Asn241 (Ala149Pro)
Accession # Q9BX67
Specificity
Detects human JAM‑C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75% cross-reactivity with recombinant mouse (rm) JAM-C is observed and less than 1% cross-reactivity with recombinant human JAM-1 and rmJAM-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
JAM3
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 2-8 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1189 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JAM-C Antibody [Unconjugated]

  • CD323
  • JAM-2
  • JAM3
  • JAMC
  • JAM-C
  • JAM-CFLJ14529
  • junctional adhesion molecule 3JAM-3
  • junctional adhesion molecule C

Background

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-C cDNA predicts a 310 amino acid (aa) residue precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 46 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3, 4). Human JAM-C shares 86% aa sequence identity with its mouse homologue. It also shares approximately 36% and 32% aa sequence homology with human JAM-B and JAM-A, respectively (3‑5). Human JAM-C shows widespread tissue expression and the highest levels are found in the placenta, brain, kidney and heart. JAM-C is expressed on endothelial cells of high endothelial venules in human tonsil. It is also expressed on platelets, T-cells and NK cells (3‑5). Unlike other JAM family members, JAM-C forms only weak homotypic interactions. JAM-C binds to JAM-B to facilitate the interactions between JAM-B and the integrin alpha4beta1 (6). This heterotypic interaction between leukocyte JAM-C and endothelial JAM-B may play a role in regulating leukocyte transmigration (5). On platelets, JAM-C is a counter-receptor for the leukocyte integrin Mac-1(CD11b/CD18) (7). JAM-C has also been identified as a strong candidate gene for hypoplastic left heart syndrome (8).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B. Under this system, JAM-C refers to the protein encoded by the gene localized to human chromosome 11.

  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Arrate, M.P. et al. (2001) J. Biol. Chem. 276:45826.
  4. Liang, T. et al. (2002) J. Immunol. 168:1618.
  5. Johnson-Leger, C. et al. (2002) Blood 100:25793.
  6. Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
  7. Santoso, S. et al. (2002) J. Exp. Med. 196:679.
  8. Phillips, H.M. et al. (2002) Genomics 79:475.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00050848-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA, WB
AF1074
Species: Hu, Mu
Applications: IHC, Neut, Simple Western, WB
AF1730
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, ICC
NB110-89474
Species: Bv, Ce, ChHa, Hu, Pm, Mu, Rt, RM
Applications: Dual ISH-IHC, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P, ISH, Simple Western, Single-Cell Western, WB
AF3449
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB
NBP1-85047
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
H00050848-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA, WB
AF1008
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
NBP2-02259
Species: Hu, Mu, Po, Rt
Applications: Flow, ICC/IF
NBP1-87402
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
AF3628
Species: Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, WB
NBP1-88861
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
7268-CT
Species: Hu
Applications: BA
NBP1-88192
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
137-PS
Species: Hu
Applications: BA
796-IC
Species: Mu
Applications: BA

Publications for JAM-C Antibody (AF1189)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Neutralization, Western Blot.


Filter By Application
Neutralization
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF1189 Applications Species
J Li, X Tan, Q Sun, X Li, R Chen, L Luo Deficiency of Jamc Leads to Congenital Nuclear Cataract and Activates the Unfolded Protein Response in Mouse Lenses Investigative Ophthalmology & Visual Science, 2022;63(10):1. 2022 [PMID: 36048019] (Western Blot, Mouse) Western Blot Mouse
LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions PloS Pathogens, 2022;18(1):e1010280. 2022 [PMID: 35100311] (Western Blot, Human) Western Blot Human
Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin. Immunology, 2009;128(2):196-205. 2009 [PMID: 19740376] (Neutralization, Mouse) Neutralization Mouse
Fuse C, Ishida Y, Hikita T, Asai T, Oku N Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma. J. Biol. Chem., 2007;282(11):8276-83. 2007 [PMID: 17227766] (Western Blot, Human) Western Blot Human
Zen K, Babbin BA, Liu Y, Whelan JB, Nusrat A, Parkos CA JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration. Mol. Biol. Cell, 2004;15(8):3926-37. 2004 [PMID: 15194813] (Western Blot, Human) Western Blot Human

Reviews for JAM-C Antibody (AF1189) (0)

There are no reviews for JAM-C Antibody (AF1189). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for JAM-C Antibody (AF1189) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional JAM-C Products

Bioinformatics Tool for JAM-C Antibody (AF1189)

Discover related pathways, diseases and genes to JAM-C Antibody (AF1189). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for JAM-C Antibody (AF1189)

Discover more about diseases related to JAM-C Antibody (AF1189).
 

Pathways for JAM-C Antibody (AF1189)

View related products by pathway.

PTMs for JAM-C Antibody (AF1189)

Learn more about PTMs related to JAM-C Antibody (AF1189).

Blogs on JAM-C

There are no specific blogs for JAM-C, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our JAM-C Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol JAM3
Uniprot