JAM-C Antibodies

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF017). A specific band was detected for JAM‑C at approximately 36-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for JAM‑C at approximately 36-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human placenta tissue, human brain tissue, JAR human choriocarcinoma cell line, and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human JAM‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for JAM‑C at approximately 36-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

1074-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Human JAM-B Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189). The ND₅₀ is typically 2-8 µg/mL." class="big_lightbox"> 1074-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Human JAM-B Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189). The ND₅₀ is typically 2-8 µg/mL." class="big_thumb" />

HAF018). A specific band was detected for JAM-C at approximately 36-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for JAM-C at approximately 36-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human placenta tissue, human brain tissue, JAR human choriocarcinoma cell line, and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human JAM-C Monoclonal Antibody (Catalog # MAB1189) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for JAM-C at approximately 36-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Human JAM-B/VE-JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human JAM-C Monoclonal Antibody (Catalog # MAB1189). The ND₅₀ is typically 0.01-0.05 µg/mL." class="big_lightbox"> G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Human JAM-B/VE-JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human JAM-C Monoclonal Antibody (Catalog # MAB1189). The ND₅₀ is typically 0.01-0.05 µg/mL." class="big_thumb" />

MAB0041) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB0041) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="Human platelets were stained with anti-human CD41 FITC-conjugated antibody and either (A) Mouse Anti-Human JAM-C Monoclonal Antibody (Catalog # MAB11891) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />