JAM-B/VE-JAM Antibody [Unconjugated]

Images

 
Western blot shows lysates of bEnd.3 mouse endothelioma cell line and SVEC4‑10 mouse vascular endothelial cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse JAM‑B/VE‑JAM Antigen ...read more
Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse JAM‑B/VE‑JAM Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of human placenta tissue and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for JAM‑B/VE‑JAM at approximately 58‑61 kDa (as indicated) ...read more
JAM‑B/VE‑JAM was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human/Mouse JAM‑B/VE‑JAM Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Recombinant Human JAM‑B/VE‑JAM Fc Chimera (Catalog # 1074‑VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G‑102‑C), supports the adhesion of the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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JAM-B/VE-JAM Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM‑B/VE-JAM
Phe29-Asn236
Accession # P57087
Specificity
Detects human JAM‑B/VE-JAM in direct ELISAs. Detects human and mouse JAM-B/VE-JAM in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
JAM2
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/mL
  • Simple Western 20 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Neutralization 0.2-0.8 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1074 in the following applications:

Publications
Read Publications using
AF1074 in the following applications:

  • ICC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JAM-B/VE-JAM Antibody [Unconjugated]

  • C21orf43
  • CD322 antigen
  • CD322
  • JAM2
  • JAMB
  • JAM-B
  • JAM-BJAM-IT/VE-JAM
  • junctional adhesion molecule 2JAM-2
  • junctional adhesion molecule B
  • PRO245
  • Vascular endothelial junction-associated molecule
  • VE-JAM
  • VE-JAMVEJAMCD322

Background

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. JAM-B, alternatively named vascular endothelial JAM (VE-JAM), is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It is also expressed on the endothelium of a variety of non-lymphoid organs, especially the heart and placenta (3, 5). Human JAM-B cDNA predicts a 298 amino acid residue (aa) precursor protein with a putative 28 aa signal peptide, a 209 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 38 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site. Human JAM-B shares approximately 79% aa sequence homology with its mouse homologue. It also shares approximately 35% aa sequence homology with human JAM-A or JAM-C. JAM-B exhibits homotypic interactions, as well as heterotypic interactions with JAM-C, but not JAM-A (4, 5, 7). It is also a ligand for the integrin alpha 4 beta 1. However, the JAM-B/ alpha 4 beta 1 interaction is facilitated only after prior adhesion of JAM-B to JAM-C (6). Through its heterotypic interactions with JAM-C, JAM-B is an adhesive ligand for T, NK, and dendritic cells, and may play a role in regulating leukocyte transmigration (5).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred in the literature variously as JAM-B or JAM-3. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Palmeri, A. et al. (2000) J. Biol. Chem. 275:19139.
  4. Cunnigham, S. et al. (2000) J. Biol. Chem. 275:34750.
  5. Liang, T. et al. (2002) J. Immunol. 168:1618.
  6. Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
  7. Arrate, M. et al. (2001) J. Biol. Chem. 276:45826.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JAM-B/VE-JAM Antibody (AF1074)(2)

Review for JAM-B/VE-JAM Antibody (AF1074) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1074:
Filter by Applications
Flow
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 01/05/2015
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Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 19795504
SpeciesHuman

Blocking

Blocking DetailsSee PMID 19795504

Details

Detection NotesSee PMID 19795504

Comments

CommentsPublished PMID 19795504

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Recent Reviews

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Anonymous
01/05/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol JAM2
Entrez
Uniprot