JAM-A Antibody (2E3-1C8) - Azide and BSA Free

Immunohistochemistry: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Coronal sections of human meninges including the retrobulbar, midorbital, and intracanalicular part of the optic nerve stained for cell-cell interaction markers of tight junctions (JAM-A, Occludin, and Claudin 5). A representative staining of one patient is shown. * indicates arachnoid layer. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fneur.2017.00308/full) licensed under a CC-BY license.

Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody (M01), clone 2E3-1C8. Lane 1: F11R transfected lysate (Predicted MW: 32.6 KDa). Lane 2: Non-transfected lysate.

Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - F11R monoclonal antibody (M01), clone 2E3-1C8 Analysis of F11R expression in HepG2.

Immunohistochemistry-Paraffin: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of monoclonal antibody to F11R on formalin-fixed paraffin-embedded human breast cancer tissue. Antibody concentration 1 ug/ml.

ELISA: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Detection limit for recombinant GST tagged F11R is approximately 0.3ng/ml as a capture antibody.

Proximity Ligation Assay: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of protein-protein interactions between PRKCZ and F11R. HeLa cells were stained with anti-PRKCZ rabbit purified polyclonal 1:1200 and anti-F11R mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Detection limit for recombinant GST tagged F11R is approximately 0.1ng/ml as a capture antibody.

Immunohistochemistry-Paraffin: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Sagittal sections of human meninges including the retrobulbar, midorbital, & intracanalicular part of the optic nerve stained for cell–cell interaction markers of tight junctions (JAM1, Occludin, & Claudin 5), gap junctions (Connexin 26 & 43), & desmosomes (Desmoplakin). A representative staining of one patient is shown. * indicates arachnoid layer. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28706505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Coronal sections of human meninges including the retrobulbar, midorbital, & intracanalicular part of the optic nerve stained for cell–cell interaction markers of tight junctions (JAM1, Occludin, & Claudin 5), gap junctions (Connexin 26 & 43), & desmosomes (Desmoplakin). A representative staining of one patient is shown. * indicates arachnoid layer. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28706505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF, IHC
• Clone: 2E3-1C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

JAM-A Antibody (2E3-1C8) - Azide and BSA Free Summary

• Immunogen: F11R (AAH01533, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGTKAQVERKLLCLFILAILLCSLALGSVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVIVAAVLVTLILLGILVFGIWFAYSRGHFDRTKKGTSSKKVIYSQPSARSEGEFKQTSSFLV
• Localization: Type I membrane protein
• Specificity: F11R - F11 receptor
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: F11R
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for JAM-A Antibody (2E3-1C8) - Azide and BSA Free

• CD321 antigen
• CD321
• F11 receptor
• F11R
• JAM-1
• JAM1CD321
• JAMA
• JAM-A
• JCAMJAM
• Junctional adhesion molecule 1Platelet F11 receptor
• junctional adhesion molecule A
• PAM-1
• PAM-1KAT
• Platelet adhesion molecule 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JAM-A Antibody (H00050848-M01)(11)

Publications using H00050848-M01

• Tom H, Matteo G, Nicole D et al. Gastrointestinal cancer-associated fibroblasts expressing Junctional Adhesion Molecule-A are amenable to infection by oncolytic reovirus. Cancer Gene Ther. 2022-07-22 [PMID: 35869278]
• Cathy R, Katherine S, Elaine K et al. Development of a Novel Weighted Ranking Method for Immunohistochemical Quantification of a Heterogeneously Expressed Protein in Gastro-Esophageal Cancers. Cancers (Basel). 2021-03-13 [PMID: 33805812]
• Laudine C, Mauricio M, Fabrice S et al. Low Junctional Adhesion molecule-A Expression Is Associated With an Epithelial to Mesenchymal Transition and Poorer Outcomes in High-Grade Serous Carcinoma of Uterine Adnexa. Mod Pathol. 2020-06-08 [PMID: 32514162]
• Neutzner A, Power L, Durrenberger M et al. A perfusion bioreactor-based 3D model of the subarachnoid space based on a meningeal tissue construct Fluids Barriers CNS 2019-06-13 [PMID: 31189484] (IF/IHC, Human)
• Baek K and Choi Y. Estrogen reinforces barrier formation and protects against tumor necrosis factor alpha-induced barrier dysfunction in oral epithelial cells. J Periodontal Implant Sci. 2018-10-24 [PMID: 30405936]
• Vellanki SH, Cruz RGB, Jahns H et al. Natural compound Tetrocarcin-A downregulates Junctional Adhesion Molecule-A in conjunction with HER2 and Inhibitor of Apoptosis Proteins and inhibits tumor cell growth. Cancer Lett 2018-10-09 [PMID: 30312728]
• Zeleny TNC, Kohler C, Neutzner A et al. Cell-Cell Interaction Proteins (Gap Junctions, Tight Junctions, and Desmosomes) and Water Transporter Aquaporin 4 in Meningothelial Cells of the Human Optic Nerve Front Neurol 2017-07-14 [PMID: 28706505] (IHC-P, Human)
• Huang JY, Xu YY, Sun Z et al. Low JAM-A expression correlates with poor prognosis in gastric cancer. Journal of Surgical Research. 2014-01-01 [PMID: 25033702]
• Fong D, Spizzo G, Mitterer M et al. Low Expression of Junctional Adhesion Molecule A Is Associated with Metastasis and Poor Survival in Pancreatic Cancer. Ann Surg Oncol. 2012-05-02 [PMID: 22549289]
• McSherry EA, McGee SF, Jirstrom K et al. JAM-A expression positively correlates with poor prognosis in breast cancer patients. Int J Cancer. 2009-09-15 [PMID: 19533747]
• Gutwein P, Schramme A, Voss B et al. Downregulation of junctional adhesion molecule-A is involved in the progression of clear cell renal cell carcinoma. Biochem Biophys Res Commun 380(2):387-91. 2009-03-06 [PMID: 19250634]

Bioinformatics

• Gene Symbol: F11R
• Entrez:
  • 50848(Human)
• OMIM:
  • 605721(Human)
• Uniprot:
  • AAH01533(Human)