JAM-A Antibody (2E3-1C8)

Images

 
Immunohistochemistry: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Coronal sections of human meninges including the retrobulbar, midorbital, and intracanalicular part of the optic nerve stained for cell-cell interaction ...read more
Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody (M01), clone 2E3-1C8. Lane 1: F11R transfected lysate (Predicted MW: 32.6 ...read more
Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - F11R monoclonal antibody (M01), clone 2E3-1C8 Analysis of F11R expression in HepG2.
Immunohistochemistry-Paraffin: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of monoclonal antibody to F11R on formalin-fixed paraffin-embedded human breast cancer tissue. Antibody concentration 1 ug/ml.
ELISA: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Detection limit for recombinant GST tagged F11R is approximately 0.3ng/ml as a capture antibody.
Proximity Ligation Assay: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of protein-protein interactions between PRKCZ and F11R. HeLa cells were stained with anti-PRKCZ rabbit purified polyclonal 1:1200 and ...read more
Sandwich ELISA: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Detection limit for recombinant GST tagged F11R is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA
Clone
2E3-1C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

JAM-A Antibody (2E3-1C8) Summary

Immunogen
F11R (AAH01533, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGTKAQVERKLLCLFILAILLCSLALGSVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVIVAAVLVTLILLGILVFGIWFAYSRGHFDRTKKGTSSKKVIYSQPSARSEGEFKQTSSFLV
Localization
Type I membrane protein
Specificity
F11R - F11 receptor
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
F11R
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using
H00050848-M01 in the following applications:

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for JAM-A Antibody (2E3-1C8)

  • CD321 antigen
  • CD321
  • F11 receptor
  • F11R
  • JAM-1
  • JAM1CD321
  • JAMA
  • JAM-A
  • JCAMJAM
  • Junctional adhesion molecule 1Platelet F11 receptor
  • junctional adhesion molecule A
  • PAM-1
  • PAM-1KAT
  • Platelet adhesion molecule 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JAM-A Antibody (H00050848-M01)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, IHC-P.


Filter By Application
IHC
(1)
IHC-P
(1)
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Filter By Species
Human
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using H00050848-M01 Applications Species
Neutzner A, Power L, Durrenberger M et al. A perfusion bioreactor-based 3D model of the subarachnoid space based on a meningeal tissue construct Fluids Barriers CNS Jun 13 2019 [PMID: 31189484] (IHC, Human) IHC Human
Baek K and Choi Y. Estrogen reinforces barrier formation and protects against tumor necrosis factor alpha-induced barrier dysfunction in oral epithelial cells. J Periodontal Implant Sci. 2018 Oct 24 [PMID: 30405936]
Vellanki SH, Cruz RGB, Jahns H et al. Natural compound Tetrocarcin-A downregulates Junctional Adhesion Molecule-A in conjunction with HER2 and Inhibitor of Apoptosis Proteins and inhibits tumor cell growth. Cancer Lett Oct 9 2018 [PMID: 30312728]
Zeleny TNC, Kohler C, Neutzner A et al. Cell-Cell Interaction Proteins (Gap Junctions, Tight Junctions, and Desmosomes) and Water Transporter Aquaporin 4 in Meningothelial Cells of the Human Optic Nerve Front Neurol 2017 Jul 14 [PMID: 28706505] (IHC-P, Human) IHC-P Human
Huang JY, Xu YY, Sun Z et al. Low JAM-A expression correlates with poor prognosis in gastric cancer. Journal of Surgical Research. 2014 [PMID: 25033702]
Fong D, Spizzo G, Mitterer M et al. Low Expression of Junctional Adhesion Molecule A Is Associated with Metastasis and Poor Survival in Pancreatic Cancer. Ann Surg Oncol. 2012 May 02 [PMID: 22549289]
McSherry EA, McGee SF, Jirstrom K et al. JAM-A expression positively correlates with poor prognosis in breast cancer patients. Int J Cancer. 2009 Sep 15 [PMID: 19533747]
Gutwein P, Schramme A, Voss B et al. Downregulation of junctional adhesion molecule-A is involved in the progression of clear cell renal cell carcinoma. Biochem Biophys Res Commun 380(2):387-91. 2009 Mar 6. [PMID: 19250634]

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Bioinformatics

Gene Symbol F11R
Entrez
OMIM
Uniprot