Jak1 Antibody (JM75-03)

Western Blot: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of JAK1 on different lysates with Rabbit anti-JAK1 antibody at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: HT-29 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 133 kDa Observed band size: 133 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-JAK1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of SW480 cells with JAK 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of JAK1 on Hela cell using anti-JAK1 antibody at 1/1,000 dilution.Positive control:Lane 1: PC-12 Lane 2: JurkatLane 3: NIH-3T3

Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded mouse colon tissue using anti-JAK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded mouse kidney tissue using anti-JAK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-JAK1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Jak1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: JM75-03
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Jak1 Antibody (JM75-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Jak1 aa 621-670 / 1,154. (SwissProt: P23458 Human; SwissProt: P52332 Mouse; SwissProt: G3V9W2 Rat)
• Localization: Endomembrane system.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: JAK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Western Blot 1:500-1:1000
• Theoretical MW: 133 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Jak1 Antibody (JM75-03)

• EC 2.7.10
• Jak1
• JAK-1
• JAK1Atyrosine-protein kinase JAK1
• JAK1B
• Janus kinase 1EC 2.7.10.2
• JTK3

Background

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterised by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signalling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jak1 Antibody (NBP2-75547)(1)

Publication using NBP2-75547

• Al-Ani B, ShamsEldeen AM, Kamar SS et al. Lipopolysaccharide induces acute lung injury and alveolar hemorrhage in association with the cytokine storm, coagulopathy and AT1R/JAK/STAT augmentation in a rat model that mimics moderate and severe Covid-19 pathology Clinical and experimental pharmacology & physiology 2022-01-23 [PMID: 35066912] (WB, Rat)

Bioinformatics

• Gene Symbol: JAK1