Jak1 Antibodies
We offer JAK1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each JAK1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our JAK1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the JAK1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our JAK1 polyclonal antibodies and browse our JAK1 monoclonal antibody catalog.
Alternate Names for Jak1 Antibodies
anti-Jak1 antibody, anti-EC 2.7.10 antibody, anti-JAK-1 antibody, anti-JAK1Atyrosine-protein kinase JAK1 antibody, anti-JAK1B antibody, anti-Janus kinase 1EC 2.7.10.2 antibody, anti-JTK3 antibody
10 Results for "jak1" in Primary Antibodies
![Western Blot: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of JAK1 on different lysates with Rabbit anti-JAK1 antibody at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: HT-29 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 133 kDa Observed band size: 133 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Jak1-Antibody-JM75-03-Western-Blot-NBP2-75547-img0005.jpg "Western Blot: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of JAK1 on different lysates with Rabbit anti-JAK1 antibody at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: HT-29 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 133 kDa Observed band size: 133 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-JAK1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak1-Antibody-JM75-03-Immunohistochemistry-Paraffin-NBP2-75547-img0008.jpg)
![Flow Cytometry: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of SW480 cells with JAK 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Jak1-Antibody-JM75-03-Flow-Cytometry-NBP2-75547-img0001.jpg)
![Western Blot: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of JAK1 on Hela cell using anti-JAK1 antibody at 1/1,000 dilution.Positive control:Lane 1: PC-12 Lane 2: JurkatLane 3: NIH-3T3](http://images.novusbio.com/fullsize/Jak1-Antibody-JM75-03-Western-Blot-NBP2-75547-img0004.jpg)
![Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded mouse colon tissue using anti-JAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Jak1-Antibody-JM75-03-Immunohistochemistry-Paraffin-NBP2-75547-img0002.jpg)
![Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded mouse kidney tissue using anti-JAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Jak1-Antibody-JM75-03-Immunohistochemistry-Paraffin-NBP2-75547-img0003.jpg)
![Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-JAK1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak1-Antibody-JM75-03-Immunohistochemistry-Paraffin-NBP2-75547-img0006.jpg)
![Immunohistochemistry-Paraffin: Jak1 Antibody (JM75-03) [NBP2-75547] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Jak1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak1-Antibody-JM75-03-Immunohistochemistry-Paraffin-NBP2-75547-img0007.jpg)
![Western Blot: Jak1 Antibody (7Q1B2) [NBP3-15436] - Western blot analysis of extracts of Mouse lung cells, using Jak1 antibody (NBP3-15436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Jak1-Antibody-7Q1B2-Western-Blot-NBP3-15436-img0004.jpg "Western Blot: Jak1 Antibody (7Q1B2) [NBP3-15436] - Western blot analysis of extracts of Mouse lung cells, using Jak1 antibody (NBP3-15436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Jak1 Antibody (7Q1B2) [NBP3-15436] - Immunohistochemistry of paraffin-embedded mouse spleen using Jak1 Rabbit mAb (NBP3-15436) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Jak1-Antibody-7Q1B2-Immunohistochemistry-Paraffin-NBP3-15436-img0003.jpg)
![Immunohistochemistry-Paraffin: Jak1 Antibody (7Q1B2) [NBP3-15436] - Immunohistochemistry of paraffin-embedded rat ovary using Jak1 Rabbit mAb (NBP3-15436) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Jak1-Antibody-7Q1B2-Immunohistochemistry-Paraffin-NBP3-15436-img0001.jpg)
![Immunohistochemistry-Paraffin: Jak1 Antibody (7Q1B2) [NBP3-15436] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Jak1 Rabbit mAb (NBP3-15436) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Jak1-Antibody-7Q1B2-Immunohistochemistry-Paraffin-NBP3-15436-img0002.jpg)
![Immunohistochemistry-Paraffin: Jak1 [p Tyr1022] Antibody [NB100-82005] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82005(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-82005_rabbit-polyclonal-jak1-p-tyr1022-antibody-113202594526.jpg "Immunohistochemistry-Paraffin: Jak1 [p Tyr1022] Antibody [NB100-82005] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82005(left) or the same antibody preincubated with blocking peptide (right).")
![Western Blot: Jak1 [p Tyr1022] Antibody [NB100-82005] - Analysis of extracts from HT29 cells untreated or treated with UV using NB100-82005.](http://images.novusbio.com/fullsize/nb100-82005_rabbit-polyclonal-jak1-p-tyr1022-antibody-10320252347264.jpg)
![Western Blot: Jak1 [p Tyr1022, p Tyr1023] Antibody [NBP3-05436] - Western blot analysis of extracts of RAW264.7 cells, using Jak1 antibody (NBP3-05436) at 1:1000 dilution.RAW264.7 cells were treated by Pervanadate (1 mM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Jak1 [p Tyr1022- p Tyr1023] Antibody-Western Blot-NBP3-05436-img0002.jpg "Western Blot: Jak1 [p Tyr1022, p Tyr1023] Antibody [NBP3-05436] - Western blot analysis of extracts of RAW264.7 cells, using Jak1 antibody (NBP3-05436) at 1:1000 dilution.RAW264.7 cells were treated by Pervanadate (1 mM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Jak1 [p Tyr1022, p Tyr1023] Antibody [NBP3-05436] - Immunofluorescence analysis of NIH/3T3 cells using Jak1 antibody (NBP3-05436) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Jak1-[p-Tyr1022--p-Tyr1023]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05436-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Jak1 [p Tyr1022, p Tyr1023] Antibody - BSA Free [Jak1] - Immunofluorescence analysis of NIH/3T3 cells using Jak1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05436_rabbit-polyclonal-jak1-p-tyr1022-p-tyr1023-antibody-52202517235729.jpg)
![Western Blot: Jak1 [p Tyr 1034] Antibody (SR1138) [NBP3-21610] - Western blot analysis using NBP3-21610 in Ramos treated with pervanadate cell lysate.](http://images.novusbio.com/fullsize/nbp3-21610_rabbit-jak1-p-tyr-1034-mab-sr1138-672023106257.jpg "Western Blot: Jak1 [p Tyr 1034] Antibody (SR1138) [NBP3-21610] - Western blot analysis using NBP3-21610 in Ramos treated with pervanadate cell lysate.")
![Immunohistochemistry: Jak1 Antibody (7A5) [NBP3-26188] - Image of Jak1 Antibody (7A5) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26188_rabbit-jak1-mab-7a5-27220248573316.jpg "Immunohistochemistry: Jak1 Antibody (7A5) [NBP3-26188] - Image of Jak1 Antibody (7A5) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
