Tyk2 Antibody (307214) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Tyk2 Monoclonal Antibody (Catalog # MAB3427) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tyk2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu
• Applications: WB
• Clone: 307214
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Tyk2 Antibody (307214) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Tyk2
  Pro2-Leu51
  Accession # P29597
• Specificity: Detects human Tyk2 in Western blots.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: TYK2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tyk2 Antibody (307214) [Unconjugated]

• EC 2.7.10
• EC 2.7.10.2
• JTK1
• non-receptor tyrosine-protein kinase TYK2
• Tyk2
• tyrosine kinase 2

Background

Tyk2 belongs to the Janus family of intracellular tyrosine kinases that are activated by receptors for many cytokines. Activation of Tyk2 occurs via phosphorylation at two adjacent tyrosine residues, Y1054 and Y1055, within the kinase domain. Tyk2 is required for interferon beta -mediated tyrosine phosphorylation of STAT3 and interferon receptor 1. Mice lacking Tyk2 are susceptible to parasitic infection but resistant to autoimmune arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Tyk2 Antibody (MAB3427) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Lei Nie WB Human 04/04/2020

Summary

• Application: Western Blot
• Sample Tested: Human breast cancer cell line SKBR3 and Positive control
• Species: Human
• Lot: XNV031603A

Bioinformatics

• Gene Symbol: TYK2
• Uniprot:
  • P29597()