Human ITK ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL
Sensitivity
0.063 ng/mL

Order Details

Human ITK ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
0.156 - 10 ng/mL
Sensitivity
0.063 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ITK

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Human ITK ELISA Kit (Colorimetric)

  • EC 2.7.10
  • EC 2.7.10.2
  • EMT
  • EMTMGC126257
  • homolog of mouse T-cell itk/tsk
  • IL2-inducible T-cell kinase
  • ITK
  • Kinase EMT
  • LYK
  • LYKMGC126258
  • PSCTK2
  • T-cell-specific kinase
  • Tcsk
  • tyrosine-protein kinase ITK/TSK
  • Tyrosine-protein kinase Lyk

Background

Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for ITK ELISA Kit (NBP3-42682). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol ITK