ITK Antibody (2F12)

Product Discontinued

ITK Antibody (2F12) Summary

• Immunogen: N-terminal for ITK protein was used as the immunogen for this antibody.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITK
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blot 2 ug/ml

Packaging, Storage & Formulations

• Storage: Lyophilized antibody can be kept at 4C for up to 3 months and should be kept at -20C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20C) or for long-term storage (-80C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 0.1 ml water.

Alternate Names for ITK Antibody (2F12)

• EC 2.7.10
• EC 2.7.10.2
• EMT
• EMTMGC126257
• homolog of mouse T-cell itk/tsk
• IL2-inducible T-cell kinase
• ITK
• Kinase EMT
• LYK
• LYKMGC126258
• PSCTK2
• T-cell-specific kinase
• Tcsk
• tyrosine-protein kinase ITK/TSK
• Tyrosine-protein kinase Lyk

Background

Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITK
• Uniprot:
  • Q08881(Human)