IRP2 Antibody [FITC]

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, GS, ICC/IF, IHC, GS
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

IRP2 Antibody [FITC] Summary

• Immunogen: A synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). [UniProt Q811J3]
• Localization: Cytoplasmic
• Predicted Species: Rat (94%), Primate (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IREB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Gel Super Shift Assays
  • Gel Supershift Assay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Preliminary results have shown poor reactivity against human protein. Immunogen displays the following percentage of sequence identity for non-tested species: primate (94%) and rat (94%) proteins. Human reactivity reported in scientific literature (PMID: 28888202).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for IRP2 Antibody [FITC]

• ACO3
• EC 4.2.1.3
• FLJ23381
• IRE-BP 2
• Iron regulatory protein 2
• iron-responsive element binding protein 2
• iron-responsive element-binding protein 2
• IRP2IRP2AD

Background

Iron regulatory proteins -1 and -2 (IRP1, IRP2) are homologous cytoplasmic RNA-binding proteins that controls cellular iron metabolism and maintain systemic iron homeostasis in vertebrates along with serving some additional iron-independent functions. IRP2 belongs to aconitase/IPM isomerase family and it binds to IRES (iron-responsive elements), which are stem-loop structures found in 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IRP2-IRE binding results in the repression of its mRNA translation and its binding to transferrin receptor mRNA inhibits the degradation of this otherwise quickly degradable mRNA. IRP2 is a substrate of FBXL5, an F-box protein that assembles together with Skp1, Cul1 and Rbx1 into an E3 ubiquitin ligase complex. When iron/O2 are high, IRP2 gets ubiquitinated/degraded by SCF complex containing FBXL5, whereas under conditions of low iron/O2, FBXL5 is degraded preventing IRP2 ubiquitination leading to its RNA-binding activity. IRP2 deficient mice depicts microcytosis, misregulated iron metabolism (in CNS, intestine as well as liver associated with FTH1 and FTL up-regulation) and iron deficiency in splenic macrophages associated with down-regulation of FTH1, FTL, and FPN1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IREB2