Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)

Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red).

Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against HEK293 (1) and CHRNA3 (AA: 32-240)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of Hela cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red).

ELISA: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 6D3A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human CHRNA3 (AA: 32-240) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHRNA3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 57.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free

• cholinergic receptor, nicotinic, alpha 3
• cholinergic receptor, nicotinic, alpha polypeptide 3
• CHRNA3
• LNCR2
• MGC104879
• NACHRA3
• neuronal acetylcholine receptor subunit alpha-3
• neuronal nicotinic acetylcholine receptor, alpha3 subunit
• Nicotinic Acetylcholine R alpha 3
• Nicotinic Acetylcholine Ra3
• PAOD2

Background

This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (NBP2-61673)(1)

Publication using NBP2-61673

• Wei N, Chu Y, Liu H Et al. Antagonistic Mechanism of alpha-Conotoxin BuIA toward the Human alpha3beta2 Nicotinic Acetylcholine Receptor ACS chemical neuroscience 2021-11-05 [PMID: 34738810]

FAQs for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (NBP2-61673). (Showing 1 - 1 of 1 FAQ).

1. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
   • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Bioinformatics

• Gene Symbol: CHRNA3