Description | NB100-2324 is useful for the detection of endogenous total IRE1 Alpha protein. For detecting phospho-IRE1 Alpha (Ser-724), we suggest NB100-2323 and it is recommended to normalize phospho-IRE1 alpha band intensity/immunoreactivity with total-IRE1 alpha using NB100-2324 or NB110-59971. |
Immunogen | This IRE1 alpha antibody was raised against a synthetic peptide within the human IRE1 alpha protein (within residues 700-800). [Swiss-Prot #O75460] |
Localization | Endoplasmic reticulum |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | ERN1 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | In Western blot a band at ~110 kDa is observed. In ICC/IF endoplasmic reticulum staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Knockout Validated reported in scientific literature (PMID: 31159306). |
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Theoretical MW | 110 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at -20C. Avoid freeze-thaw cycles. |
Buffer | Tris-Glycine, 0.15M NaCl |
Preservative | 0.05% Sodium Azide |
Concentration | 1 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Anonymous |
ICC | Mouse | 11/19/2018 |
Summary
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reviewed by:
Anonymous |
WB | Mouse | 03/07/2018 |
Summary
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Human Pancreas Whole Tissue Lysate (Adult Whole Normal) | |
Human Tonsil Whole Tissue Lysate (Adult Whole Normal) | |
IRE1 alpha Knockout HeLa Cell Lysate |
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NB100-2324AF350 | |
Alexa Fluor 405 | NB100-2324AF405 | |
Alexa Fluor 488 | NB100-2324AF488 | |
Alexa Fluor 532 | NB100-2324AF532 | |
Alexa Fluor 594 | NB100-2324AF594 | |
Alexa Fluor 647 | NB100-2324AF647 | |
Alexa Fluor 700 | NB100-2324AF700 | |
Alexa Fluor 750 | NB100-2324AF750 | |
Biotin | NB100-2324B | |
DyLight 350 | NB100-2324UV | |
DyLight 405 | NB100-2324V | |
DyLight 488 | NB100-2324G | |
DyLight 550 | NB100-2324R | |
DyLight 594 | NB100-2324DL594 | |
DyLight 650 | NB100-2324C | |
DyLight 680 | NB100-2324FR | |
DyLight 755 | NB100-2324IR | |
FITC | NB100-2324F | |
HRP | NB100-2324H | |
Janelia Fluor 549 | NB100-2324JF549 | |
Janelia Fluor 646 | NB100-2324JF646 |
Diseases for IRE1 alpha Antibody (NB100-2324)Discover more about diseases related to IRE1 alpha Antibody (NB100-2324).
| Pathways for IRE1 alpha Antibody (NB100-2324)View related products by pathway.
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Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U... Read full blog post. |
IRE1 alpha dependent apoptotic-signaling pathway Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg... Read full blog post. |
IRE1 alpha (inositol-requiring enzyme 1 alpha) The unfolded protein response (UPR) is a eukaryotic cell process that addresses ER stress. UPR is initiated by three ER-localized sensors: PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 alpha (... Read full blog post. |
IRE1 alpha and ER Stress: Keys to Disease Progression Pathways Inositol Requiring Enzyme 1 alpha (IRE1 alpha) is a transmembrane-RNase with an endoplasmic reticulum (ER) luminal sensor domain and cytosolic kinase and ribonuclease domains. IRE1 also plays a central role in the ER stress response (1). In a recent s... Read full blog post. |
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Anonymous 11/19/2018 |
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Application: | ICC | |
Species: | Mouse |
Anonymous 03/07/2018 |
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Application: | WB | |
Species: | Mouse |
Gene Symbol | ERN1 |