IRE1 alpha Antibody

Images

 
Immunohistochemistry: IRE1 alpha Antibody [NB100-2324] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 ...read more
Genetic Strategies: Western Blot: IRE1 alpha Antibody [NB100-2324] - Total IRE1 Alpha protein in lysates from Min6 cells which were transfected with GFP-siRNA or Ire1-siRNA. Theoretical molecular weight: 110 kDa.
Western Blot: IRE1 alpha Antibody [NB100-2324] - ALOX15 knockout blocks alcohol-induced up-regulation of IRE1a in mouse liver. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody [NB100-2324] - IRE-1 alpha antibody was tested in HeLa cells at 1:200 against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against ...read more
Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NB100-2324] - Analysis of a formalin fixed mouse colon tissue section using total IRE1 alpha antibody (NB100-2324) at 1:300. Note a strong cytoplasmic signal in ...read more
Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NB100-2324] - Analysis of a formalin fixed mouse colon tissue section using total IRE1 alpha antibody (NB100-2324) at 1:300. Note a strong cytoplasmic signal in ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

IRE1 alpha Antibody Summary

Description
NB100-2324 is useful for the detection of endogenous total IRE1 Alpha protein. For detecting phospho-IRE1 Alpha (Ser-724), we suggest NB100-2323 and it is recommended to normalize phospho-IRE1 alpha band intensity/immunoreactivity with total-IRE1 alpha using NB100-2324 or NB110-59971.
Immunogen
This IRE1 alpha antibody was raised against a synthetic peptide within the human IRE1 alpha protein (within residues 700-800). [Swiss-Prot #O75460]
Localization
Endoplasmic reticulum
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:250
  • Immunohistochemistry 1:250 - 1:500
  • Immunohistochemistry-Paraffin 1:250 - 1:500
  • Knockdown Validated
Application Notes
In Western blot a band at ~110 kDa is observed. In ICC/IF endoplasmic reticulum staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Knockout Validated reported in scientific literature (PMID: 31159306).
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Pancreas Whole Tissue Lysate (Adult Whole Normal)
Human Tonsil Whole Tissue Lysate (Adult Whole Normal)
IRE1 alpha Knockout HeLa Cell Lysate
Control Peptide
IRE1 alpha Protein (NB100-2324PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-2324 in the following applications:

Publications
Read Publications using
NB100-2324 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Notes

Take a look at IRE1 alpha Antibody Sampler Pack [NBP2-50067] if you want to try 25ug aliquots of phospho-IRE1 alpha (Ser724) Antibody [NB100-2323SS] and total IRE1 alpha Antibody [NB100-2324SS] before purchasing 100ug full vials.

Alternate Names for IRE1 alpha Antibody

  • EC 2.7.11
  • endoplasmic reticulum to nucleus signaling 1
  • Endoplasmic reticulum-to-nucleus signaling 1
  • ER to nucleus signalling 1
  • FLJ30999
  • hIRE1p
  • inositol-requiring 1
  • Inositol-requiring protein 1
  • IRE 1a
  • IRE1a
  • ire1-alpha
  • IRE1MGC163279
  • IRE1P
  • MGC163277
  • protein kinase/endoribonuclease
  • serine/threonine-protein kinase/endoribonuclease IRE1

Background

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and upregulates ER molecular chaperones in order to cope with ER stress. UPR is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha) (1). IRE1 alpha is correlated with X-box binding protein (XBP1) as a potent UPR transcriptional activator (2).

Transcriptional activation of UPR-responsive genes are regulated by the ATF6 and IRE1-XBP1 pathways, which are often regulated by HIFs and contribute to cell survival under ER hypoxic stress (3). UPR signaling can a) inhibit protein translation to restore cell function, b) activate signaling to increase production of molecular chaperones for protein folding, and c) initiate ubiquitination signaling that leads to degradation of misfolded proteins in ER.

IRE1 alpha acts as the sensor of unfolded proteins in the ER. IRE1 alpha not only promotes cell survival but can initiate apoptosis when accumulation of unfolded proteins in the ER causes stress. IRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. When detecting phospho-IRE1 alpha, it is recommended to normalize its band intensity with total IRE1 alpha.

References

1. Zheng, W., Xie, W., Yin, D., Luo, R., Liu, M., & Guo, F. (2019). ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling. Cell Commun Signal, 17(1), 42. doi:10.1186/s12964-019-0353-3

2. Cho, Y. M., Kim, D. H., Lee, K. H., Jeong, S. W., & Kwon, O. J. (2018). The IRE1alpha-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARgamma activity. Exp Mol Med, 50(8), 102. doi:10.1038/s12276-018-0131-0

3. Xia, Z., Wu, S., Wei, X., Liao, Y., Yi, P., Liu, Y.,... Liu, J. (2019). Hypoxic ER stress suppresses beta-catenin expression and promotes cooperation between the transcription factors XBP1 and HIF1alpha for cell survival. J Biol Chem, 294(37), 13811-13821. doi:10.1074/jbc.RA119.008353

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2324
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD

Publications for IRE1 alpha Antibody (NB100-2324)(34)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC-P, Knockdown Validated, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(2)
Knockdown Validated
(1)
WB
(26)
All Applications
Filter By Species
Human
(9)
Mouse
(16)
Rat
(5)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NB100-2324 Applications Species
Ding R, Ou W, Chen C et al. Endoplasmic reticulum stress and oxidative stress contribute to neuronal pyroptosis caused by cerebral venous sinus thrombosis in rats: involvement of TXNIP/peroxynitrite-NLRP3 inflammasome activation Neurochem. Int. Sep 24 2020 [PMID: 32980492] (WB, Rat) WB Rat
Srinivasan MP, Bhopale KK, Caracheo AA et al. Activation of AMP-activated protein kinase attenuates ethanol-induced ER/oxidative stress and lipid phenotype in human pancreatic acinar cells Biochem Pharmacol Oct 20 2020 [PMID: 32717227] (WB, Human, Rat) WB Human, Rat
Cicalese S, Okuno K, Elliott KJ et al. 78 kDa Glucose-Regulated Protein Attenuates Protein Aggregation and Monocyte Adhesion Induced by Angiotensin II in Vascular Cells Int J Mol Sci Jul 15 2020 [PMID: 32679678] (WB, Rat) WB Rat
Garufi A, Federici G, Gilardini Montani Ms et Al. Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation Biomolecules Mar 3 2020 [PMID: 32138264] (WB, Human) WB Human
Han L, Bittner S, Dong D et al. Molecular changes in hepatic metabolism in ZDSD rats-A new polygenic rodent model of obesity, metabolic syndrome, and diabetes Biochim Biophys Acta Mol Basis Dis Jan 24 2020 [PMID: 31987840] (WB, Rat) WB Rat
Schmit K, Chen JW, Ayama-Canden S et al. Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin Cell Death Dis Dec 4 2019 [PMID: 31801939] (WB, Human) WB Human
Gu R, Huang T, Xiao J et al. The IRE1 alpha Arm of UPR Regulates Muscle Cells Immune Characters by Restraining p38 MAPK Activation Front Physiol 2019 Sep 19 [PMID: 31607947] (WB, Mouse) WB Mouse
Srinivasan MP, Bhopale KK, Amer SM et al. Linking Dysregulated AMPK Signaling and ER Stress in Ethanol-Induced Liver Injury in Hepatic Alcohol Dehydrogenase Deficient Deer Mice Biomolecules Oct 2 2019 [PMID: 31581705] (WB, Mouse) WB Mouse
Xie W, Xie J, Vince R, More SS Guanabenz Attenuates Acetaminophen-Induced Liver Toxicity and Synergizes Analgesia in Mice Chem. Res. Toxicol. Sep 30 2019 [PMID: 31524377]
Kaphalia L, Srinivasan MP, Kakumanu RD et al. Ethanol exposure impairs AMPK signaling and phagocytosis in human alveolar macrophages: Role of ethanol metabolism Alcohol. Clin. Exp. Res. Jun 18 2019 [PMID: 31211863]
Show All 34 Publications.

Reviews for IRE1 alpha Antibody (NB100-2324) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB100-2324:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry IRE1 alpha NB100-2324
Enlarge
4
reviewed by:
Anonymous
ICC Mouse 11/19/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse vascular smooth muscle cell
SpeciesMouse
LotD-1
Western Blot IRE1 alpha NB100-2324
Enlarge
5
reviewed by:
Anonymous
WB Mouse 03/07/2018
View

Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesMouse
Lotc1

Product General Protocols

View specific protocols for IRE1 alpha Antibody (NB100-2324): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRE1 alpha Antibody (NB100-2324). (Showing 1 - 2 of 2 FAQs).

  1. I have a customer who is interested in antibody NB100-2324 for use with rat samples. It is listed as working with rat and for Western blot applications. Can you tell me which rat tissues were tested in Western blot?
    • Previous productions were reported to work in rat. We have not tested the current lot directly on any rat samples so I do not have data on what tissues, or lysates were effective. We will still guarantee rat as a reactive species, as it is stated on our datasheet.
  2. We hope to know which position the antibody NB 100-2324 recognize within the endogenous IRE1 alpha. Thanks. Is the region 700 to 800 within the luminal domain, or transmembrane or cytosolic domain? Is this region highly conserved between Human and mouse?
    • It looks like the 700-800 region is cytoplasmic. The homology in this region is 93%, and this product has already been validated to work in both human and mouse species.https://www.uniprot.org/uniprot/O75460

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2324AF350
Alexa Fluor 405 NB100-2324AF405
Alexa Fluor 488 NB100-2324AF488
Alexa Fluor 532 NB100-2324AF532
Alexa Fluor 594 NB100-2324AF594
Alexa Fluor 647 NB100-2324AF647
Alexa Fluor 700 NB100-2324AF700
Alexa Fluor 750 NB100-2324AF750
Biotin NB100-2324B
DyLight 350 NB100-2324UV
DyLight 405 NB100-2324V
DyLight 488 NB100-2324G
DyLight 550 NB100-2324R
DyLight 594 NB100-2324DL594
DyLight 650 NB100-2324C
DyLight 680 NB100-2324FR
DyLight 755 NB100-2324IR
FITC NB100-2324F
HRP NB100-2324H
Janelia Fluor 549 NB100-2324JF549
Janelia Fluor 646 NB100-2324JF646

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Blogs on IRE1 alpha.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

IRE1 alpha (inositol-requiring enzyme 1 alpha)
The unfolded protein response (UPR) is a eukaryotic cell process that addresses ER stress. UPR is initiated by three ER-localized sensors: PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 alpha (...  Read full blog post.

IRE1 alpha and ER Stress: Keys to Disease Progression Pathways
Inositol Requiring Enzyme 1 alpha (IRE1 alpha) is a transmembrane-RNase with an endoplasmic reticulum (ER) luminal sensor domain and cytosolic kinase and ribonuclease domains. IRE1 also plays a central role in the ER stress response (1). In a recent s...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
11/19/2018
Application: ICC
Species: Mouse

Anonymous
03/07/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ERN1