IRAK1 Antibody

Images

 
Western Blot: IRAK1 Antibody [NB100-56699] - IRAK Antibody [NB100-56699] - Analysis of IRAK-1 in 1) human HeLa and 2) mouse NIH3T3 lysate using IRAK-1 antibody at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IRAK1 Antibody Summary

Immunogen
Rabbit anti-IRAK polyclonal antibody was raised against a peptide corresponding to amino acids 700 to 712 of human IRAK.
Specificity
An 80 kDa band is observed. The is human, mouse, and rat (based on sequence) reactive, and has no cross reactivity to IRAK2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IRAK1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 5 ug/ml
  • Immunoprecipitation 2-4 ug per sample
Positive Control
NIH 3T3 Lysate (NB800-PC8)
HeLa Lysate (NB800-PC1)

Reactivity Notes

Cross reacts with Mouse, Rat and Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for IRAK1 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • Il1rak
  • interleukin-1 receptor-associated kinase 1
  • IRAK1
  • IRAK-1
  • IRAKPelle homolog
  • Pelle
  • Plpk

Background

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (3,4). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (2). IRAK mediates a signaling cascade leading to NF-kB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
NB100-56583
Species: Hu
Applications: WB, Flow, IHC-P
NBP2-26506
Species: Hu, Mu, Rt
NBP2-29328
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA
NB500-597
Species: Hu, Mu, Rt
Applications: WB, Flow, IP
NB100-56722
Species: Hu, Ca
Applications: WB, B/N, Flow, ICC/IF, IHC-P, IP, Flow-CS
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
NB100-56447
Species: Hu, Mu
Applications: WB
NBP1-76782
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-24729
Species: Hu, Mu, Rt, Ca, Eq, Mk, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, In vitro, CyTOF-ready, Flow-IC
AF8691
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, KO
NB100-56699
Species: Hu, Mu, Rt
Applications: WB, IP
Supplier Logo

Publications for IRAK1 Antibody (NB100-56699) (0)

There are no publications for IRAK1 Antibody (NB100-56699).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IRAK1 Antibody (NB100-56699) (0)

There are no reviews for IRAK1 Antibody (NB100-56699). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IRAK1 Antibody (NB100-56699) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for IRAK1 Antibody (NB100-56699)

Discover related pathways, diseases and genes to IRAK1 Antibody (NB100-56699). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IRAK1 Antibody (NB100-56699)

Discover more about diseases related to IRAK1 Antibody (NB100-56699).
 

Pathways for IRAK1 Antibody (NB100-56699)

View related products by pathway.

PTMs for IRAK1 Antibody (NB100-56699)

Learn more about PTMs related to IRAK1 Antibody (NB100-56699).
 

Research Areas for IRAK1 Antibody (NB100-56699)

Find related products by research area.

Blogs on IRAK1

There are no specific blogs for IRAK1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IRAK1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol IRAK1
Entrez
Uniprot