Integrin beta 5 Antibody - BSA Free

Western Blot: Integrin beta 5 Antibody [NBP2-16976] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 5 antibody [N1C1] (NBP2-16976) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Integrin beta 5 Antibody [NBP2-16976] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 5 antibody [N1C1] (NBP2-16976) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Integrin beta 5 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Integrin beta 5. The exact sequence is proprietary.
• Predicted Species: Mouse (98%), Rat (98%), Porcine (98%), Bovine (98%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITGB5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (89%), Xenopus laevis (80%), Zebrafish (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Integrin beta 5 Antibody - BSA Free

• FLJ26658
• Integrin beta 5
• integrin beta-5
• integrin, beta 5
• ITGB5

Background

Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ITGB5
• Uniprot:
  • P18084()