Integrin beta 5 Antibody [Biotin]

Integrin beta 5 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Integrin beta 5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to transmembrane proteins. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Integrin beta 5 Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta 5
  Gly24-Asn719
  Accession # P18084
• Specificity: Detects human Integrin beta 5 in Western blots. In Western blots, less than 5% cross-reactivity with Integrin beta 1, -beta 2, -beta 3, recombinant mouse Integrin beta 4, -beta 6, and -beta 7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITGB5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 5 Antibody [Biotin]

• FLJ26658
• Integrin beta 5
• integrin beta-5
• integrin, beta 5
• ITGB5

Background

Integrin  beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin  alpha _V. Integrin  alpha _V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin  beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin  beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin  beta 5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 5 Antibody (BAF3824)(1)

Publication using BAF3824

• JV Schiesser, T Loudovaris, HE Thomas, AG Elefanty, EG Stanley Integrin &alphav&beta5 heterodimer is a specific marker of human pancreatic beta cells Scientific Reports, 2021-04-15;11(1):8315. 2021-04-15 [PMID: 33859325] (Flow Cytometry, ICC, IHC, Human)

Bioinformatics

• Gene Symbol: ITGB5
• Uniprot:
  • P18084()