Integrin beta 4/CD104 [p Tyr1526] Antibody

Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 [p Tyr1526] Antibody [NBP3-23401] - Immunocytochemical labeling of Integrin beta 4/CD104 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-Integrin beta 4/CD104 (Cytoplasmic region) (left) or rabbit polyclonals anti-Integrin beta 4/CD104 (Tyr-1494) (middle) or anti-Integrin beta 4/CD104 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight(R) 594.

Western Blot: Integrin beta 4/CD104 [p Tyr1526] Antibody [NBP3-23401] - Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin beta 4/CD104 (Tyr-1526) (lanes 1 & 2) and anti-Integrin beta 4/CD104 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin beta 4/CD104 (lanes 5 & 6).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Integrin beta 4/CD104 [p Tyr1526] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-Integrin beta 4/CD104 (Tyr-1526) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1526 of human Integrin beta 4/CD104. This peptide sequence is found in all three Integrin beta 4/CD104 isoforms and is highly conserved in rat and mouse Integrin beta 4/CD104. [UniProt# P16144]
• Modification: p Tyr1526
• Specificity: This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to unphosphorylated Integrin beta 4/CD104 (Tyr-1526) peptide before affinity purification using phospho-Integrin beta 4/CD104 (Tyr-1526) peptide (without carrier). The purified antibody detects a 200kDa protein corresponding to the molecular mass of Integrin beta 4/CD104 on SDS-PAGE immunoblots of A431 and src-transformed Hct116 cells treated with pervanadate, but not in control cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITGB4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 200 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Integrin beta 4/CD104 [p Tyr1526] Antibody

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB4