Integrin beta 4/CD104 Antibody (450-9D) - BSA Free

Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) [NB100-65599] - Staining of human tonsil cryostat section with Mouse anti human CD104

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: 450-9D
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Integrin beta 4/CD104 Antibody (450-9D) - BSA Free Summary

• Description: Novus Biologicals Mouse Integrin beta 4/CD104 Antibody (450-9D) - BSA Free (NB100-65599) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-Integrin beta 4/CD104 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified alpha6 beta4 integrin from A431 cells
• Specificity: NB100-65599 recognizes the human beta4 integrin (also known as CD104), a 205kD glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumour cell lines. NB100-65599 recognizes an extracellular epitope on the CD104 molecule.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Reactivity Notes

Predicted cross-reactivities: Ferret, Mink, Mustelid

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Integrin beta 4/CD104 Antibody (450-9D) - BSA Free

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 4/CD104 Antibody (NB100-65599)(5)

Publications using NB100-65599

• Michail Raptopoulos, Nicholas G Fischer, Conrado Aparicio Implant surface physicochemistry affects keratinocyte hemidesmosome formation. Journal of biomedical materials research. Part A 2023-05-03 [PMID: 36621832]
• Sasamoto Y, Lee CAA, Wilson BJ et al. Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation Cell reports 2022-08-09 [PMID: 35947947] (IF/IHC, Human)
  
  Details:
  Dilutions: 1:200
• Fischer NG, Kobe AC, Dai J et al. Tapping basement membrane motifs: Oral junctional epithelium for surface-mediated soft tissue attachment to prevent failure of percutaneous devices Acta biomaterialia 2021-12-29 [PMID: 34971784] (ICC/IF)
• Fischer G, He J, Aparicio C Surface Immobilization Chemistry of a LamininDerived Peptide Affects Keratinocyte Activity Coatings (Basel) 2020-06-11 [PMID: 32855816] (ICC/IF, Human)
• Tra WM, van Neck JW, Hovius SE et al. Characterization of a three-dimensional mucosal equivalent: similarities and differences with native oral mucosa. Cells Tissues Organs 2012-01-01 [PMID: 21494020]

Review for Integrin beta 4/CD104 Antibody (NB100-65599) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 03/14/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Human Oral Keratinocyte
• Species: Human
• Lot: 0515

Comments

• Comments: 1:1000 dilution

Bioinformatics

• Gene Symbol: ITGB4
• Entrez:
  • 3691(Human)
• Uniprot:
  • P16144()
  • P16144()