Integrin beta 4/CD104 Antibody (422325) [Unconjugated]

Integrin beta 4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4060) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 422325
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 4/CD104 Antibody (422325) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 4/CD104
  Asn28-Ser710
  Accession # P16144
• Specificity: Detects human Integrin beta 4/CD104 in Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, beta 2, beta 3, beta 5, beta 6, beta 8, or recombinant mouse Integrin beta 4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 4/CD104 Antibody (422325) [Unconjugated]

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 (also GP150 and CD104) is a 150‑200 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta 4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. Two ECD alternative splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta 4 ECD shares 88% aa sequence identity with mouse beta 4 ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 4/CD104 Antibody (MAB4060)(12)

Publications using MAB4060

• L Pontiggia, AK Ahuja, HK Yosef, D Rütsche, E Reichmann, U Moehrlen, T Biedermann Human Basal and Suprabasal Keratinocytes Are Both Able to Generate and Maintain Dermo-Epidermal Skin Substitutes in Long-Term In Vivo Experiments Cells, 2022-07-09;11(14):. 2022-07-09 [PMID: 35883599] (IHC, Human)
• DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression The Journal of Biological Chemistry, 2022-05-13;0(0):102029. 2022-05-13 [PMID: 35569509] (Immunoprecipitation, Western Blot, Human)
• Hara T, Kasagi Y, Wang J et al. CD73+ epithelial progenitor cells which contribute to homeostasis and renewal are depleted in eosinophilic esophagitis Cellular and molecular gastroenterology and hepatology 1/30/2022 [PMID: 35108658]
• CR Keller, Y Hu, KF Ruud, AE VanDeen, SR Martinez, BT Kahn, Z Zhang, RK Chen, W Li Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells Cancers, 2021-11-22;13(22):. 2021-11-22 [PMID: 34831010] (ICC, Human)
• Yang Yang, Yizhe Wang, Xiaofang Che, Kezuo Hou, Jie Wu, Chunlei Zheng et al. Integrin alpha 5 promotes migration and invasion through the FAK/STAT3/AKT signaling pathway in icotinib‑resistant non‑small cell lung cancer cells Oncology Letters 2021-05-24 [PMID: 34084223]
• R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy PLoS ONE, 2019-05-02;14(5):e0215990. 2019-05-02 [PMID: 31048929] (ELISA Detection, Human)
• Rijal G, Wang J, Yu I et al. Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture. Int J Mol Sci. 9/25/2018 [PMID: 30257480]
• A Rajan, BD Persson, L Frängsmyr, A Olofsson, L Sandblad, J Heino, Y Takada, AP Mould, LM Schnapp, J Gall, N Arnberg Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells Sci Rep, 2018-07-03;8(1):10019. 2018-07-03 [PMID: 29968781] (Flow Cytometry)
• V Russo, T Klein, DJ Lim, N Solis, Y Machado, S Hiroyasu, L Nabai, Y Shen, MR Zeglinski, H Zhao, CP Oram, PA Lennox, N Van Laeken, NJ Carr, RI Crawford, CW Franzke, CM Overall, DJ Granville Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction Sci Rep, 2018-06-26;8(1):9690. 2018-06-26 [PMID: 29946113] (Western Blot)
• Rickard J Storm Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells J. Virol, 2017-02-14;0(0):. 2017-02-14 [PMID: 27974569] (Flow Cytometry, IHC-Fr, Neutralization, Human)
• Li X, Rossen N, Sinn P, Hornick A, Steines B, Karp P, Ernst S, Adam R, Moninger T, Levasseur D, Zabner J Integrin alpha6beta4 identifies human distal lung epithelial progenitor cells with potential as a cell-based therapy for cystic fibrosis lung disease. PLoS ONE, 2013-12-12;8(12):e83624. 2013-12-12 [PMID: 24349537] (IHC, Human)
• Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces. J. Cell. Sci., 2011-01-11;124(0):369-83. 2011-01-11 [PMID: 21224397] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGB4
• Uniprot:
  • P16144()