Integrin beta 4/CD104 Antibody (422325) [Unconjugated]

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Integrin beta 4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4060) at 25 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
422325
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Integrin beta 4/CD104 Antibody (422325) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 4/CD104
Asn28-Ser710
Accession # P16144
Specificity
Detects human Integrin beta 4/CD104 in Western blots. In Western blots, no cross-reactivity with recombinant human Integrin  beta 1, beta 2, beta 3, beta 5, beta 6, beta 8, or recombinant mouse Integrin  beta 4 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ITGB4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB4060 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 4/CD104 Antibody (422325) [Unconjugated]

  • CD104 antigen
  • CD104
  • GP150
  • Integrin beta 4
  • integrin beta-4 subunit
  • integrin beta-4
  • integrin, beta 4
  • ITGB4

Background

Integrin beta 4 (also GP150 and CD104) is a 150‑200 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta 4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. Two ECD alternative splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta 4 ECD shares 88% aa sequence identity with mouse beta 4 ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 4/CD104 Antibody (MAB4060)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Detection, Flow Cytometry, ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
ELISA Detection
(1)
Flow Cytometry
(3)
ICC
(1)
IHC
(1)
Immunoprecipitation
(1)
Western Blot
(1)
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Filter By Species
Human
(6)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB4060 Applications Species
DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression The Journal of Biological Chemistry, 2022;0(0):102029. 2022 [PMID: 35569509] (Immunoprecipitation, Human) Immunoprecipitation Human
CR Keller, Y Hu, KF Ruud, AE VanDeen, SR Martinez, BT Kahn, Z Zhang, RK Chen, W Li Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells Cancers, 2021;13(22):. 2021 [PMID: 34831010] (ICC, Human) ICC Human
R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy PLoS ONE, 2019;14(5):e0215990. 2019 [PMID: 31048929] (ELISA Detection, Human) ELISA Detection Human
A Rajan, BD Persson, L Frängsmyr, A Olofsson, L Sandblad, J Heino, Y Takada, AP Mould, LM Schnapp, J Gall, N Arnberg Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells Sci Rep, 2018;8(1):10019. 2018 [PMID: 29968781] (Flow Cytometry) Flow Cytometry
V Russo, T Klein, DJ Lim, N Solis, Y Machado, S Hiroyasu, L Nabai, Y Shen, MR Zeglinski, H Zhao, CP Oram, PA Lennox, N Van Laeken, NJ Carr, RI Crawford, CW Franzke, CM Overall, DJ Granville Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction Sci Rep, 2018;8(1):9690. 2018 [PMID: 29946113] (Western Blot) Western Blot
Rickard J Storm Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells J. Virol, 2016;0(0):. 2016 [PMID: 27974569] (Flow Cytometry, Human) Flow Cytometry Human
Li X, Rossen N, Sinn P, Hornick A, Steines B, Karp P, Ernst S, Adam R, Moninger T, Levasseur D, Zabner J Integrin alpha6beta4 identifies human distal lung epithelial progenitor cells with potential as a cell-based therapy for cystic fibrosis lung disease. PLoS ONE, 2013;8(12):e83624. 2013 [PMID: 24349537] (IHC, Human) IHC Human
Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces. J. Cell. Sci., 2011;124(0):369-83. 2011 [PMID: 21224397] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol ITGB4
Uniprot