Integrin beta 4/CD104 Antibody (422325) [Alexa Fluor® 488]

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Integrin beta 4/CD104 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB4060G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 422325
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Integrin beta 4/CD104 Antibody (422325) [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 4/CD104
  Asn28-Ser710
  Accession # P16144
• Specificity: Detects human Integrin beta 4/CD104 in Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, beta 2, beta 3, beta 5, beta 6, beta 8, or recombinant mouse Integrin beta 4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 4/CD104 Antibody (422325) [Alexa Fluor® 488]

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 (also GP150 and CD104) is a 150-200 kDa member of the integrin beta family. It forms noncovalent heterodimers with integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human integrin beta 4 is a type I transmembrane glycoptotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28-710) and a large 1089 aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621-704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta 4 ECD shares 88% aa sequence identity with mouse beta 4 ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 4/CD104 Antibody (FAB4060G)(2)

Publications using FAB4060G

• Ademi, H;Michalak-Micka, K;Moehrlen, U;Biedermann, T;Klar, AS; Effects of an Adipose Mesenchymal Stem Cell-Derived Conditioned medium and TGF-?1 on Human Keratinocytes In Vitro International journal of molecular sciences 2023-09-29 [PMID: 37834173] (Flow Cytometry, Human)
• ES Grigoryeva, LA Tashireva, OE Savelieva, MV Zavyalova, NO Popova, GA Kuznetsov, ES Andryuhova, VM Perelmuter The Association of Integrins beta3, beta4, and alphaVbeta5 on Exosomes, CTCs and Tumor Cells with Localization of Distant Metastasis in Breast Cancer Patients International Journal of Molecular Sciences, 2023-02-02;24(3):. 2023-02-02 [PMID: 36769251] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGB4
• Uniprot:
  • P16144()