Integrin beta 4/CD104 Antibody (422325) [Allophycocyanin]

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Integrin beta 4/CD104 APC-conjugated Monoclonal Antibody (Catalog # FAB4060A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 422325
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Integrin beta 4/CD104 Antibody (422325) [Allophycocyanin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 4/CD104
  Asn28-Ser710
  Accession # P16144
• Specificity: Detects human Integrin beta 4/CD104 in Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, beta 2, beta 3, beta 5, beta 6, beta 8, or recombinant mouse Integrin beta 4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8degrees C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 4/CD104 Antibody (422325) [Allophycocyanin]

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 (also GP150 and CD104) is a 150-200 kDa member of the integrin beta family of proteins. It forms noncovalent heterodimers with integrin alpha 6 and participates in the formation of epithelial hemidesmosomes by binding Plexin 1A. Integrin alpha 6 beta 4 also binds Laminin. Human integrin beta 4 is a type I transmembrane glycoptotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28-710) and a large 1089 aa cytoplasmic region. One ECD alternate splice form is reported. One shows an 84 aa substitution for the 84 aa between aa 621-704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta 4 ECD shares 88% aa sequence identity with mouse beta 4 ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB4
• Uniprot:
  • P16144()