Human Integrin beta 3/CD61 ELISA Kit (Colorimetric)

ELISA: Human Integrin beta 3/CD61 ELISA Kit (Colorimetric) [NBP3-39963] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 13.3 pg/mL (example only; lot dependent)

Human Integrin beta 3/CD61 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 31.25 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 13.3 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ITGB3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Integrin beta 3/CD61 ELISA Kit (Colorimetric)

• CD61 antigen
• CD61
• GP3A
• GP3Aplatelet glycoprotein IIIa
• GPIIIA
• GPIIIaintegrin beta-3
• INGRB3
• Integrin beta 3
• integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• ITG B3
• ITGB3
• Platelet membrane glycoprotein IIIa

Background

Platelet membrane glycoprotein Integrin beta-3 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar membrane glycoprotein complex is present on endothelial cells. Homology suggests that GP IIIa is a member of a family of cell-surface adhesion receptors. (1) Data provide further support for the key role of the cytoplasmic domain of the beta3 integrin in cell adhesion and suggest a potential role for the beta3C integrin subunit in modulating cell-matrix interactions (2). It has been demonstrated that the beta 3 subunit of alpha IIb beta 3 was phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3). be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ITGB3