Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free

Flow Cytometry: Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free [NBP1-97563] - SUM159PT cells were stained with Integrin beta 3/CD61 antibody (NB100-64861AF488, 0.5 ug/10^6 cells). Left: unstain; Right: ITGB3 antibody stain. Image from verified customer review.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PE-CyTM7.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC-CyTM7.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PE.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PerCP.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) FITC.

Separation of human CD61 positive thrombocytes (red-filled) from neutrophil granulocytes (black-dashed) in Analysis (surface staining) of peripheral whole blood stained using anti-human CD61 (VIPL2) purified antibody (concentration in sample 3 ug/ml, GAM APC).

• Reactivity: Hu, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: VIPL2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free Summary

• Description: Novus Biologicals Mouse Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free (NBP1-97563) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-Integrin beta 3/CD61 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Clone VIPL2 was used by HLDA to establish CD designation.
• Immunogen: The mouse monoclonal antibody VIPL2 recognizes CD61
• Specificity: The mouse monoclonal antibody VIPL2 recognizes CD61, a 90-110 kDa transmembrane glycoprotein of integrin family, expressed on platelets, megacaryocytes, osteoclasts, endothelial cells and other cell types, including leucocytes and smooth muscle cells.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Western Blot 1:1000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free

• CD61 antigen
• CD61
• GP3A
• GP3Aplatelet glycoprotein IIIa
• GPIIIA
• GPIIIaintegrin beta-3
• INGRB3
• Integrin beta 3
• integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• ITG B3
• ITGB3
• Platelet membrane glycoprotein IIIa

Background

CD61 (beta3 integrin) is a transmembrane glycoprotein, which associates with CD41 or CD51 molecules to form heterodimeric adhesion receptores. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. It binds to fibronectin, fibrinogen and von Willebrand factor, and is involved in platelet aggregation. CD51/CD61 complex has similar binding properties and is involved in modulating migration and survival of angiogenic endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 3/CD61 Antibody (NBP1-97563)(2)

Publications using NBP1-97563

• Tang J, Su T et al. Targeted repair of heart injury by stem cells fused with platelet nanovesicles. Nat Biomed Eng 2018-05-06 [PMID: 29862136] (FLOW, Human)
• Sousa HR, Gaspar RS, Sena EM et al. Novel antiplatelet role for a protein disulfide isomerase-targeted peptide: Evidence of covalent binding to C-terminal CGHC redox motif J. Thromb. 2017-01-21 [PMID: 28109047] (FLOW, Human)
  
  Details:
  This citation used the PerCP version of this antibody.

Bioinformatics

• Gene Symbol: ITGB3