Integrin beta 2/CD18 Antibody (YFC118.3) (NB200-610)

Images

 
Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as ...read more
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis using the Biotin conjugate of NB200-610. Staining of human peripheral blood lymphocytes.
Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of canine thoracic fluid samples using anti-Integrin beta 2 antibody. The antibody showing positive cell surface ...read more
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood lymphocytes with Rat anti Human CD18.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with Rat anti Human CD18:RPE
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) ...read more
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing high affinity conformation) and ...read more
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood lymphocytes using CD18 BIOTIN.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18: FITC.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of Biotin conjugate of NB200-610. Staining of human peripheral blood lymphocytes.

Product Details

Summary
Reactivity Hu, Ca, GPSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IP
Clone
YFC118.3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1 mg/ml

Integrin beta 2/CD18 Antibody (YFC118.3) Summary

Immunogen
Human neutrophils
Specificity
This recognises the human CD18 cell surface antigen, the integrin beta2 subunit. CD18 is expressed by the majority of leucocytes.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
ITGB2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunoprecipitation 1:10-1:500
Positive Control
Integrin beta 2/CD18 Lysate (NBL1-12072)
Reviewed Applications
Read 1 Review rated 4
using
NB200-610 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Integrin beta 2/CD18 Antibody (YFC118.3)

  • 959 beta subunit precursor)10
  • CD18 antigen
  • CD18
  • CD18beta 2
  • cell surface adhesion glycoprotein (LFA-1
  • CR3
  • Integrin beta 2
  • integrin beta-2
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • ITGB2
  • LAD
  • LCAMB
  • leukocyte cell adhesion molecule CD18
  • leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • LFA-1 beta
  • LFA-1
  • MAC-1 beta
  • MF17
  • MFI7
  • P150
  • p150,95 beta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 2/CD18 Antibody (NB200-610) (0)

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Review for Integrin beta 2/CD18 Antibody (NB200-610) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: ICC.

Reviews using NB200-610:
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ICC
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Images Ratings Applications Species Date Details
Immunocytochemistry Integrin beta 2/CD18 NB200-610
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reviewed by:
Anonymous
ICC Other 01/23/2015
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Summary

ApplicationImmunocytochemistry
SpeciesOther
Special ApplicationsWe recently validated your Integrin beta 2/CD18 (NB200-610) antibody for use with canine cytology samples via immunocytochemistry (ICC). Upon speaking with one of your technical representatives, it was confirmed that this antibody has not been tested for ICC and, thus, we qualify for the Innovator’s Reward Program. This antibody was utilized on canine thoracic fluid samples containing an abundance of macrophages (as determined via cytologic examination). Indirect/two-step ICC was performed, with a Cy3 secondary antibody directed against your primary antibody. Two photographs are provided (40X objective): a rat IgG control primary antibody demonstrating no staining (not shown on review); and your antibody showing positive cell surface signaling. Of note, this antibody appears to work best on native/unfixed cells, as fixation with 4% PFA (in PBS) removed the positive signal. We are currently testing acetone and methanol fixation, but do not, yet, have those results. We do hope that you will find these results useful and will be able to provide us with a 100% voucher to purchase another 0.1mg container. Given the success of this antibody, we intend to be consistent purchasers.
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FAQs for Integrin beta 2/CD18 Antibody (NB200-610). (Showing 1 - 1 of 1 FAQ).

  1. Can you please provide me the immunogen sequence?
    • NB200-610 is a licensed clone and all of our available information comes from the clone inventor. Unfortunately, there is no additional information on the immunogen available.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-610AF405
Alexa Fluor 488 Labeled NB200-610AF488
Alexa Fluor 647 Labeled NB200-610AF647
Alexa Fluor 700 Labeled NB200-610AF700
Allophycocyanin Labeled NB200-610APC
Biotin Labeled NB100-63503
DyLight 350 Labeled NB200-610UV
DyLight 405 Labeled NB200-610V
DyLight 488 Labeled NB200-610G
DyLight 680 Labeled NB200-610FR
DyLight 755 Labeled NB200-610IR
PE Labeled NB200-610PE
PerCP Labeled NB200-610PCP

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Blogs on Integrin beta 2/CD18.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

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Recent Reviews

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1
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Anonymous
01/23/2015
Application: ICC
Species: Other

Bioinformatics

Gene Symbol ITGB2
Entrez

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