Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

Flow Cytometry: Integrin beta 2/CD18 Antibody (MEM-48) [FITC] [NB500-380] - Surface staining of human peripheral blood with anti-CD18 (MEM-48) FITC.

• Reactivity: Hu, Ca(-)
• Applications: Flow
• Clone: MEM-48
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Integrin beta 2/CD18 Antibody (MEM-48) [FITC] Summary

• Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
• Specificity: The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB2
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 106 cells in a suspension.
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

• 959 beta subunit precursor)10
• CD18 antigen
• CD18
• CD18beta 2
• cell surface adhesion glycoprotein (LFA-1
• CR3
• Integrin beta 2
• integrin beta-2
• integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
• integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• ITGB2
• LAD
• LCAMB
• leukocyte cell adhesion molecule CD18
• leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
• LFA-1 beta
• LFA-1
• MAC-1 beta
• MF17
• MFI7
• P150
• p150,95 beta

Background

CD18, integrin a2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (a2) integrins: aLa2 (CD11a/CD18, LFA-1), aMa2 (CD11b/CD18, Mac-1, CR3), aXa2 (CD11c/CD18) and aDa2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB2