Integrin beta 1D Antibody (1G2)

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clone
1G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Integrin beta 1D Antibody (1G2) Summary

Immunogen
Clone 1G2 is a mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to the Cterminal 24 amino acids of integrin beta1D including an appending N-terminal cysteine (CQENPIYKSPINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.
Specificity
1G2 recognizes specifically the cytoplasmic domain of integrin subunit beta 1D present in cardiac and skeletal muscle.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50-1:100
Application Notes
Optimal antibody dilution should be determined by titration; recommended range is 1:50 - 1:100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Integrin beta 1D Antibody (1G2)

  • integrin beta-1
  • CD29
  • FNRB
  • GPIIA
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
  • MDF2
  • MSK12
  • VLAB
  • VLA-BETA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-97733AF405
Alexa Fluor 488 Labeled NBP1-97733AF488
Alexa Fluor 647 Labeled NBP1-97733AF647
Alexa Fluor 700 Labeled NBP1-97733AF700
DyLight 350 Labeled NBP1-97733UV
DyLight 405 Labeled NBP1-97733V
DyLight 488 Labeled NBP1-97733G
DyLight 680 Labeled NBP1-97733FR
DyLight 755 Labeled NBP1-97733IR

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Diseases for Integrin beta 1D Antibody (NBP1-97733)

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PTMs for Integrin beta 1D Antibody (NBP1-97733)

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Bioinformatics

Gene Symbol ITGB1