Integrin beta 1/CD29 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of L‑929 mouse fibroblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, PC‑12 rat adrenal pheochromocytoma cell line, and Rat‑2 rat embryonic fibroblast cell ...read more
Western blot shows lysates of L‑929 mouse fibroblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, PC‑3 human prostate cancer cell line, Rat‑2 rat embryonic fibroblast cell line, and RAW ...read more
Orexin B, p38 alpha, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Human/Mouse/Rat p38 alpha Antigen ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Integrin beta 1/CD29 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1
Gln21-Ala738
Accession # P09055
Specificity
Detects mouse Integrin beta 1/CD29 in direct ELISAs. Detects mosue and rat Integrin beta 1/CD29 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 1 and rhIntegrin beta 3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 4, and rmIntegrin beta 6 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ITGB1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Publications
Read Publications using
AF2405 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 1/CD29 Antibody [Unconjugated]

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 1/CD29 Antibody (AF2405)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: IHC, IHC Fresh, IP, WB.


Filter By Application
IHC
(2)
IHC Fresh
(1)
IP
(2)
WB
(1)
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Mouse
(5)
Rat
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF2405 Applications Species
UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 2017;19(3):189-201. 2017 [PMID: 28218908] (IHC,, Mouse) IHC Mouse
Wu, B, Cloer, C, Lu, P, Milazi, S, Shaban, M, Shah, S N, Marston-Poe, L, Moulton, H M, Lu, Q L Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice. Gene Ther, 2014;21(9):785-93. 2014 [PMID: 24942628] (IHC Fresh,, Mouse) IHC Fresh Mouse
Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5. J. Neurochem., 2011;118(4):521-32. 2011 [PMID: 21166806] (IP,, Mouse) IP Mouse
Liu S, Kapoor M, Denton CP, Abraham DJ, Leask A Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model. Arthritis Rheum., 2009;60(9):2817-21. 2009 [PMID: 19714619] (WB,, Mouse) WB Mouse
Gendronneau G, Sidhu SS, Delacour D, Dang T, Calonne C, Houzelstein D, Magnaldo T, Poirier F Galectin-7 in the control of epidermal homeostasis after injury. Mol. Biol. Cell, 2008;19(12):5541-9. 2008 [PMID: 18829868] (IHC,, Mouse) IHC Mouse
Chang Y, Finnemann SC Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis. J. Cell. Sci., 2007;120(0):3053-63. 2007 [PMID: 17684062] (IP,, Rat) IP Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Integrin beta 1/CD29 Antibody (AF2405). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Secondary Antibodies

 

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits....  Read full blog post.

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Bioinformatics

Gene Symbol ITGB1
Entrez
Uniprot