Integrin beta 1/CD29 Antibody [Unconjugated]

Western blot shows lysates of MG-63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta 1/CD29 at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin beta 1/CD29 at approximately 198 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Canine mesenchymal stem cells were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Integrin beta 1/CD29 was detected in immersion fixed canine mesenchymal stem cells using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Integrin beta 1/CD29 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Integrin beta 1/CD29 at approximately 157-176 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Integrin beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western blot shows lysates of A549 human lung carcinoma parental cell line and Integrin beta 1/CD29 knockout A549 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta 1/CD29 at approximately 105 kDa and 120 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Ca
• Applications: WB, Simple Western, Flow, CyTOF-ready, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 1/CD29 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1 isoform 1A
  Gln21-Asp728
  Accession # P05556
• Specificity: Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 10-20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 1/CD29 Antibody [Unconjugated]

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

Integrin beta 1, also called CD29 and VLA-beta chain, associates with at least ten different integrin alpha subunits to form various VLA complexes. The beta 1 subunit has a broad tissue distribution except erythrocytes. Over aa 21-720, human  Integrin beta 1 shares 95% aa identity with canine Integrin beta 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (AF1778)(7)

Publications using AF1778

• Lin, LL;Nayak, B;Osmulski, PA;Wang, E;Wang, CP;Valente, PT;Wang, CM;Tan, X;Santanam, N;Wang, TL;Gaczynska, ME;Kost, ER;Huang, TH;Kirma, NB; PAI-1 uncouples integrin-?1 from restrain by membrane-bound ?-catenin to promote collagen fibril remodeling in obesity-related neoplasms Cell reports 2024-07-23 [PMID: 39046873] (Immunocytochemistry, Functional Assay, Human)
• Kumarasamy, V;Wang, J;Frangou, C;Wan, Y;Dynka, A;Rosenheck, H;Dey, P;Abel, EV;Knudsen, ES;Witkiewicz, AK; The extracellular niche and tumor microenvironment enhance KRAS inhibitor efficacy in pancreatic cancer Cancer research 2024-01-31 [PMID: 38294344] (Western Blot, Human, Mouse)
• JM Sierra-Par, A Merino, M Eijken, H Leuvenink, R Ploeg, BK Møller, B Jespersen, CC Baan, MJ Hoogduijn Reparative effect of mesenchymal stromal cells on endothelial cells after hypoxic and inflammatory injury Stem Cell Res Ther, 2020-08-12;11(1):352. 2020-08-12 [PMID: 32787906] (Neutralization, Human)
• Sven Flemming, Anny-Claude Luissint, Dennis H. M. Kusters, Arturo Raya-Sandino, Shuling Fan, Dennis W. Zhou et al. Desmocollin-2 promotes intestinal mucosal repair by controlling integrin-dependent cell adhesion and migration Molecular Biology of the Cell 2020-03-15 [PMID: 31967937]
• X Zhou, R Zhao, K Schwarz, M Mangeat, EC Schwarz, M Hamed, I Bogeski, V Helms, H Rieger, B Qu Bystander cells enhance NK cytotoxic efficiency by reducing search time Sci Rep, 2017-03-13;7(0):44357. 2017-03-13 [PMID: 28287155] (Migration Assay, Human)
• Rickard J Storm Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells J. Virol, 2017-02-14;0(0):. 2/14/2017 [PMID: 27974569]
• Windus L, Kiss D, Glover T, Avery V In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer. Exp Cell Res, 2012-07-27;318(19):2507-19. 2012-07-27 [PMID: 22841689] (Western Blot, Human)

FAQs for Integrin beta 1/CD29 Antibody (AF1778). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1
• Uniprot:
  • P05556()