Integrin beta 1/CD29 Antibody (JB1B) - BSA Free

Product Discontinued

Integrin beta 1/CD29 Antibody (JB1B) - BSA Free Summary

• Immunogen: Purified Human beta1 integrin
• Marker: Stem Cell Marker
• Specificity: NB100-64804 reacts with Human Integrin beta1 as confirmed with beta1 transfectants and by reciprocal preclearing of beta1 reactivity with anti-beta1. The antibody stimulates the adherence of several cell types. The removal of azide is recommended prior to use in functional assays.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.1 ug/ml-1 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunoprecipitation 1-5 ug
  • Western Blot 1:100-1:2000
• Control:

  Integrin beta 1/CD29 Overexpression Lysate

Reactivity Notes

Reacts with Human. Cross reacts with Cynomolgus monkey and Rhesus Monkey. Does not react with Rat. Reacts weakly with Pig.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin beta 1/CD29 Antibody (JB1B) - BSA Free

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (NB100-64804)(1)

Publication using NB100-64804

• Bottcher RT, Strohmeyer N, Aretz J, Fassler R New insights into the phosphorylation of the threonine motif of the beta1 integrin cytoplasmic domain Life science alliance 2022-04-01 [PMID: 34996844]

FAQs for Integrin beta 1/CD29 Antibody (NB100-64804). (Showing 1 - 1 of 1 FAQ).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1
• Entrez:
  • 3688(Human)
  • 24511(Rat)
• Uniprot:
  • P05556()