Integrin alpha V/CD51 Antibody [Unconjugated]

HAF017). A specific band was detected for Integrin aV/CD51 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). A specific band was detected for Integrin aV/CD51 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin aV/CD51 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin alpha V/CD51 at approximately 150 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Integrin aV/CD51 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

Integrin aV/CD51 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." > HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." title="Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin aV/CD51 at approximately 149-161 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." />

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin aV/CD51 at approximately 149-161 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Integrin aV/CD51 at approximately 120 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). A specific band was detected for Integrin aV/CD51 at approximately 120 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of A549 human lung carcinoma parental cell line and Integrin aV/CD51 knockout A549 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin aV/CD51 at approximately 120 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of A549 human lung carcinoma parental cell line and Integrin aV/CD51 knockout A549 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " > AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " title="HT1080 cells were stained with Goat Anti-Human/Mouse/Rat Integrin  alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # af1219, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " />

HT1080 cells were stained with Goat Anti-Human/Mouse/Rat Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # af1219, filled histogram) or isotype control antibody (Catalog # <a class=NoLineLink href=

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, CyTOF-ready, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha V/CD51 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V/CD51
  Phe31-Val992
  Accession # P06756
• Specificity: Detects human Integrin alpha V in direct ELISAs. Detects human, mouse and rat Integrin alpha V in Simple Western application.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGAV
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 20 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha V/CD51 Antibody [Unconjugated]

• antigen identified by monoclonal L230
• CD51 antigen
• CD51
• Integrin alpha V
• integrin alpha-V
• integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• ITGAV
• MSK8
• Vitronectin Receptor alpha
• Vitronectin receptor subunit alpha
• VNRA
• VNRADKFZp686A08142

Background

Integrin alpha V, also known as CD51 and vitronectin receptor subunit  alpha , is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alpha V, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta -propellar domain. Furin cleavage of the alpha V ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin  alpha V shares 92% aa sequence identity with mouse Integrin  alpha V ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha V/CD51 Antibody (AF1219)(9)

Publications using AF1219

• Valeria Fernández Vallone, Francisco Raúl Borzone, Leandro Marcelo Martinez, María Belén Giorello, Hosoon Choi, Federico Dimase et al. Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients Frontiers in Oncology 2/20/2023 [PMID: 36890825]
• SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs Nature Immunology, 2022-12-01;0(0):. 2022-12-01 [PMID: 36456734] (Flow Cytometry Control, Human)
• K Kondo, Y Harada, M Nakano, T Suzuki, T Fukushige, K Hanzawa, H Yagi, K Takagi, K Mizuno, Y Miyamoto, N Taniguchi, K Kato, T Kanekura, N Dohmae, K Machida, I Maruyama, H Inoue Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells The Journal of Biological Chemistry, 2022-04-18;0(0):101950. 2022-04-18 [PMID: 35447118] (Western Blot, Human)
• H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno Platelet-derived extracellular vesicles are increased in sera of Alzheimer&#039;s disease patients, as revealed by Tim4-based assays FEBS Open Bio, 2021-02-03;0(0):. 2021-02-03 [PMID: 33345458] (Western Blot, Human)
• E Gjika, S Pal-Ghosh, ME Kirschner, L Lin, JH Sherman, MA Stepp, M Keidar Combination therapy of cold atmospheric plasma (CAP) with temozolomide in the treatment of U87MG glioblastoma cells Sci Rep, 2020-10-05;10(1):16495. 2020-10-05 [PMID: 33020527] (ICC, Human)
• Chun-Chun Cheng, Ko Tsutsui, Toru Taguchi, Noriko Sanzen, Asako Nakagawa, Kisa Kakiguchi et al. Hair follicle epidermal stem cells define a niche for tactile sensation eLife 10/25/2018 [PMID: 30355452]
• Characterization of human and murine T-cell immunoglobulin mucin domain 4 (TIM-4) IgV domain residues critical for Ebola virus entry J Virol, 2016-06-10;0(0):. 2016-06-10 [PMID: 27122575] (Flow Cytometry, Human)
• Hussain , Sajid, Rodriguez-Fernandez , Maria, Braun , Gary B, Doyle , Francis, Ruoslahti , Erkki Quantity and accessibility for specific targeting of receptors in tumours. Sci Rep, 2014-06-10;4(0):5232. 2014-06-10 [PMID: 24912981] (IHC, Human)
• Bass R, Wagstaff L, Ravenhill L, Ellis V Binding of extracellular maspin to beta1 integrins inhibits vascular smooth muscle cell migration. J. Biol. Chem., 2009-07-28;284(40):27712-20. 2009-07-28 [PMID: 19638634] (Immunoprecipitation, Western Blot, Human)

Bioinformatics

• Gene Symbol: ITGAV
• Uniprot:
  • P06756()