Integrin alpha V/CD51 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, C6 rat glioma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at ...read more
Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat ...read more
Integrin alpha V/CD51 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Human/Mouse/Rat Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) ...read more
Genetic Strategies: Western blot shows lysates of A549 human lung carcinoma parental cell line and Integrin alpha V/CD51 knockout A549 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Integrin alpha V/CD51 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin  alpha V/CD51
Phe31-Val992
Accession # P06756
Specificity
Detects human Integrin  alpha V in direct ELISAs and Western blots. Detects human, mouse and rat Integrin  alpha V in Simple Western application.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ITGAV
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 20 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF1219 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha V/CD51 Antibody [Unconjugated]

  • antigen identified by monoclonal L230
  • CD51 antigen
  • CD51
  • Integrin alpha V
  • integrin alpha-V
  • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
  • ITGAV
  • MSK8
  • Vitronectin Receptor alpha
  • Vitronectin receptor subunit alpha
  • VNRA
  • VNRADKFZp686A08142

Background

Integrin alpha V, also known as CD51 and vitronectin receptor subunit  alpha , is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alpha V, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta -propellar domain. Furin cleavage of the alpha V ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin  alpha V shares 92% aa sequence identity with mouse Integrin  alpha V ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha V/CD51 Antibody (AF1219)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(1)
Immunoprecipitation
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF1219 Applications Species
K Kondo, Y Harada, M Nakano, T Suzuki, T Fukushige, K Hanzawa, H Yagi, K Takagi, K Mizuno, Y Miyamoto, N Taniguchi, K Kato, T Kanekura, N Dohmae, K Machida, I Maruyama, H Inoue Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells The Journal of Biological Chemistry, 2022;0(0):101950. 2022 [PMID: 35447118] (Western Blot, Human) Western Blot Human
H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno Platelet-derived extracellular vesicles are increased in sera of Alzheimer's disease patients, as revealed by Tim4-based assays FEBS Open Bio, 2020;0(0):. 2020 [PMID: 33345458] (Western Blot, Human) Western Blot Human
E Gjika, S Pal-Ghosh, ME Kirschner, L Lin, JH Sherman, MA Stepp, M Keidar Combination therapy of cold atmospheric plasma (CAP) with temozolomide in the treatment of U87MG glioblastoma cells Sci Rep, 2020;10(1):16495. 2020 [PMID: 33020527] (ICC, Human) ICC Human
Characterization of human and murine T-cell immunoglobulin mucin domain 4 (TIM-4) IgV domain residues critical for Ebola virus entry J Virol, 2016;0(0):. 2016 [PMID: 27122575] (Flow Cytometry, Human) Flow Cytometry Human
Hussain , Sajid, Rodriguez-Fernandez , Maria, Braun , Gary B, Doyle , Francis, Ruoslahti , Erkki Quantity and accessibility for specific targeting of receptors in tumours. Sci Rep, 2014;4(0):5232. 2014 [PMID: 24912981] (IHC, Human) IHC Human
Bass R, Wagstaff L, Ravenhill L, Ellis V Binding of extracellular maspin to beta1 integrins inhibits vascular smooth muscle cell migration. J. Biol. Chem., 2009;284(40):27712-20. 2009 [PMID: 19638634] (Immunoprecipitation, Human) Immunoprecipitation Human

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Bioinformatics

Gene Symbol ITGAV
Uniprot