Integrin alpha V/CD51 Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, CyTOF-ready, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Integrin alpha V/CD51 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin αV/CD51
  Phe31-Val992
  Accession # P06756
• Specificity: Detects human Integrin alpha V in direct ELISAs and Western blots. Detects human, mouse and rat Integrin alpha V in Simple Western application.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha V/CD51 Antibody [Alexa Fluor® 488]

• antigen identified by monoclonal L230
• CD51 antigen
• CD51
• Integrin alpha V
• integrin alpha-V
• integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• ITGAV
• MSK8
• Vitronectin Receptor alpha
• Vitronectin receptor subunit alpha
• VNRA
• VNRADKFZp686A08142

Background

Integrin alpha V, also known as CD51 and vitronectin receptor subunit  alpha , is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alpha V, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta -propellar domain. Furin cleavage of the alpha V ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin  alpha V shares 92% aa sequence identity with mouse Integrin  alpha V ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.