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Integrin alpha V/CD51 Antibody (P2W7)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
P2W7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2618AF350
Alexa Fluor 488
NB100-2618AF488
Alexa Fluor 532
NB100-2618AF532
Alexa Fluor 594
NB100-2618AF594
Alexa Fluor 750
NB100-2618AF750
Allophycocyanin
NB100-2618APC
Allophycocyanin/Cy7
NB100-2618APCCY7
Biotin
NB100-2618B
DyLight 350
NB100-2618UV
DyLight 405
NB100-2618V
DyLight 488
NB100-2618G
DyLight 550
NB100-2618R
DyLight 594
NB100-2618DL594
DyLight 650
NB100-2618C
DyLight 680
NB100-2618FR
DyLight 755
NB100-2618IR
FITC
NB100-2618F
HRP
NB100-2618H
Janelia Fluor 549
NB100-2618JF549
Janelia Fluor 646
NB100-2618JF646
PE/Atto594
NB100-2618PEATT594
PE/Cy5.5
NB100-2618PECY55
PE/Cy7
NB100-2618PECY7
PerCP
NB100-2618PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80817
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Integrin alpha V/CD51 Antibody (P2W7) Summary

Immunogen
An ocular melanoma cell line (V+B2) expressing high levels of human integrin alpha V beta 2.
Localization
Type I membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGAV
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 8-25 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa. This antibody is CyTOF ready.
Theoretical MW
155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB100-2618 in the following applications:

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Integrin alpha V/CD51 Antibody (P2W7)

  • antigen identified by monoclonal L230
  • CD51 antigen
  • CD51
  • Integrin alpha V
  • integrin alpha-V
  • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
  • ITGAV
  • MSK8
  • Vitronectin Receptor alpha
  • Vitronectin receptor subunit alpha
  • VNRA
  • VNRADKFZp686A08142

Background

Integrin alpha V chain interacts with the integrin beta 3 subunit/CD61 to form the alpha-V-beta-3 heterodimer/vitronectin receptor. It is expressed on endothelial cells, some activated leukocytes, NK cells, macrophages, neutrophils, and platelets. Integrin alpha V also forms heterodimers with the integrin beta 1, beta 5, beta 6, and beta 8 subunits. Alpha-V-beta-3 is an activation dependent receptor for platelet attachment and spreading on vitronectin and other matrix components.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Antibody [Unconjugate
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EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(9 Reviews)
(34 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC

Publications for Integrin alpha V/CD51 Antibody (NB100-2618)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Integrin alpha V/CD51 Antibody (NB100-2618) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2618AF350
Alexa Fluor 488 NB100-2618AF488
Alexa Fluor 532 NB100-2618AF532
Alexa Fluor 594 NB100-2618AF594
Alexa Fluor 750 NB100-2618AF750
Allophycocyanin NB100-2618APC
Allophycocyanin/Cy7 NB100-2618APCCY7
Biotin NB100-2618B
DyLight 350 NB100-2618UV
DyLight 405 NB100-2618V
DyLight 488 NB100-2618G
DyLight 550 NB100-2618R
DyLight 594 NB100-2618DL594
DyLight 650 NB100-2618C
DyLight 680 NB100-2618FR
DyLight 755 NB100-2618IR
FITC NB100-2618F
HRP NB100-2618H
Janelia Fluor 549 NB100-2618JF549
Janelia Fluor 646 NB100-2618JF646
PE/Atto594 NB100-2618PEATT594
PE/Cy5.5 NB100-2618PECY55
PE/Cy7 NB100-2618PECY7
PerCP NB100-2618PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ITGAV
Entrez