Integrin alpha V beta 3 Antibody (SC56-07)

Western Blot: Integrin alpha V beta 3 Antibody (SC56-07) [NBP2-67557] - Western blot analysis of Integrin alpha V beta 3 on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 ho

Immunocytochemistry/Immunofluorescence: Integrin alpha V beta 3 Antibody (SC56-07) [NBP2-67557] - ICC staining of Integrin alpha V beta 3 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed w

Flow Cytometry: Integrin alpha V beta 3 Antibody (SC56-07) [NBP2-67557] - Analysis of Integrin alpha V was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: Integrin alpha V beta 3 Antibody (SC56-07) [NBP2-67557] - ICC staining of Integrin alpha V beta 3 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed w

Product Discontinued

Integrin alpha V beta 3 Antibody (SC56-07) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within of Human Integrin alpha V beta 3 aa 1000-1048 / 1048. (SwissProt: P06756 Human; SwissProt: P43406 Mouse)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGAV
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha V beta 3 Antibody (SC56-07)

• antigen identified by monoclonal L230
• CD51 antigen
• CD51
• Integrin alpha V beta 3
• integrin alpha-V
• integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• MSK8
• Vitronectin receptor subunit alpha
• VNRADKFZp686A08142

Background

Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha V beta 3 is the predominant integrin of osteoclasts (bone resorbing cells) and is upregulated in malignant melanoma. Integrin alpha V beta 3 binds to a range of ECM proteins (e.g. vitronectin, fibrinogen, fibronectin) via an Arg-Gly-Asp (RGD) motif.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha V beta 3 Antibody (NBP2-67557)(5)

Publications using NBP2-67557

• Cammalleri M, Dal Monte M, Locri F et al. The urokinase-type plasminogen activator system as drug target in retinitis pigmentosa: New pre-clinical evidence in the rd10 mouse model J. Cell. Mol. Med. 2019-08-01 [PMID: 31251468] (WB, Mouse)
• Gregory JV, Kadiyala P, Doherty R, Cadena M Systemic Brain Tumor Delivery of Synthetic Protein Nanoparticles for Glioblastoma Therapy bioRxiv
• Ratajczak-Wielgomas K, Kmiecik A, Dziegiel P Role of Periostin Expression in Non-Small Cell Lung Cancer: Periostin Silencing Inhibits the Migration and Invasion of Lung Cancer Cells via Regulation of MMP-2 Expression International Journal of Molecular Sciences 2022-01-22 [PMID: 35163164] (WB)
• Veerasubramanian P, Shao H, Meli V Et al. A Src-H3 acetylation signaling axis integrates macrophage mechanosensation with inflammatory response Biomaterials 2021-10-01 [PMID: 34753038] (ICC/IF, Mouse)
• Gregory J, Kadiyala P, Doherty R et al. Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy Nat Commun 2020-12-01 [PMID: 33173024] (FLOW, Mouse)
  
  Details:
  GL26-OVA glioblastoma cells were harvested from tumors in mice and underwent flow cytometry.

Bioinformatics

• Gene Symbol: ITGAV