Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free

Flow Cytometry: Rat Monoclonal Integrin alpha 4/CD49d Antibody (R1-2) [NBP3-09131] - Flow cytometry surface staining pattern of murine peripheral blood cells stained using anti-mouse CD49d (R1-2) purified antibody (concentration in sample 5 ug/ml, DAR APC).

Flow Cytometry - Rat Monoclonal Integrin alpha 4/CD49d Antibody (R1-2) [NBP3-09131] - Separation of cells stained using anti-mouse CD49d (R1-2) purified antibody (concentration in sample 5 μg/ml, DAR APC, red-filled) from cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of murine peripheral blood cells.

• Reactivity: Mu
• Applications: Flow, IHC, IP
• Clone: R1-2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free Summary

• Immunogen: murine T lymphoma line TK1
• Specificity: The rat monoclonal antibody R1-2 recognizes an extracellular epitope of murine CD49d / Integrin alpha 4 (VLA-4 alpha), an approximately 150 kDa glycoprotein of the integrin family, expressed on multiple blood cell types.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry-Frozen
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Integrin alpha 4/CD49d Antibody (R1-2) - BSA Free

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• ITGA4
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha
• VLA-4

Background

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA4