Integrin alpha 4/CD49d Antibody (HP2/1) - BSA Free

Flow Cytometry: Integrin alpha 4/CD49d Antibody (HP2/1) [NB120-22858] - A surface stain was performed on Jurkat Cells with Integrin alpha 4/CD49d Antibody (HP2/1) NB120-22858B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

• Reactivity: Hu
• Applications: Flow, Func, IHC, IP
• Clone: HP2/1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha 4/CD49d Antibody (HP2/1) - BSA Free Summary

• Description: Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63 Ag8.653 myeloma cell line.
• Additional Information: Clone HP2/1 was used by HLDA to establish CD designation.
• Immunogen: JM leukaemia line
• Specificity: Mouse anti Human CD49d monoclonal antibody, clone HP2/1 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997). Mouse anti human CD49d, clone HP2/1 binds to both intact and the 80kDa fragment of integrin alpha-4. CD49d is expressed on monocytes, T cells, B cells, thymocytes and Langerhans cells (de Graaf et al. 1995). Mouse anti Human CD49d, clone HP2/1 can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and has been demonstrated to inhibit cell binding to soluble VCAM-1 (Weller et al. 1991).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1ug/5 x 10^5 cells
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.

Reactivity Notes

Predicted cross-reactivities: Rhesus Monkey, Mustelid, Rat, Rabbit, Porcine, Llama, Mink, Equine, Goat, Feline, Cynomolgus monkey, Bovine

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin alpha 4/CD49d Antibody (HP2/1) - BSA Free

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• ITGA4
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha
• VLA-4

Background

Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA4
• Entrez:
  • 3676(Human)
  • 311144(Rat)
• Uniprot:
  • P13612()
  • P13612()