Integrin alpha 2b/CD41 Antibody (2E10C9) - BSA Free

Western Blot: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Western blot analysis using ITGA2B mAb against HEK293 (1) and ITGA2B (AA: 45-149)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ITGA2B mouse mAb with DAB staining.

Flow Cytometry: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Flow cytometric analysis of HL60 cells using ITGA2B mouse mAb (green) and negative control (purple).

Western Blot: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Western blot analysis using ITGA2B mAb against human ITGA2B recombinant protein. (Expected MW is 36.9 kDa)

Immunohistochemistry: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ITGA2B mouse mAb with DAB staining.

ELISA: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 2E10C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha 2b/CD41 Antibody (2E10C9) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Integrin alpha 2b/CD41 (AA: 45-149) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA2B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 113.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Integrin alpha 2b/CD41 Antibody (2E10C9) - BSA Free

• CD41 antigen
• CD41
• CD41BHPA3
• GP2B
• GP2Bintegrin alpha-IIb
• GPalpha IIb
• GPIIb
• GTA
• HPA3
• Integrin alpha 2b
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41)
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41B)
• ITGA2b
• ITGAB
• platelet fibrinogen receptor, alpha subunit
• Platelet membrane glycoprotein IIb
• platelet-specific antigen BAK

Background

Integrin alpha 2b (CD41) is a calcium-dependent, noncovalently associated heterodimer and contains a heavy chain (GPIIb alpha) and a light chain (GPIIb beta) linked by a single disulfide bond. The Integrin alpha 2B chain interacts with the Integrin beta 3 subunit/CD61 to form the platelet glycoprotein complex, gpIIb/IIIa. It is expressed on platelets and megakaryocytes. Ligands for the gpIIb/IIIa heterodimer include fibrinogen, von Willebrand factor, fibronectin, vitronectin, and thrombospondin. The gpIIb/IIIa complex is the major Integrin on platelets and is important for platelet adhesion and aggregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA2B