Integrin alpha 1/CD49a Antibody (CL7207) - BSA Free

Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7207) [NBP2-76478] - Analysis in human prostate and skeletal muscle tissues. Corresponding ITGA1 RNA-seq data are presented for the same tissues.

Western Blot: Integrin alpha 1/CD49a Antibody (CL7207) [NBP2-76478] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7207) [NBP2-76478] - Staining of human placenta shows strong membranous positivity in blood vessels and loose connective tissue of chorionic villi.

Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7207) [NBP2-76478] - Staining of human prostate shows strong membranous positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7207) [NBP2-76478] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7207) [NBP2-76478] - Staining of human stomach shows moderate membranous positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC, B/N, KD
• Clone: CL7207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Integrin alpha 1/CD49a Antibody (CL7207) - BSA Free Summary

• Immunogen: This antibody was developed using a synthetic peptide derived from P56199, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Block/Neutralize reported in scientific literature (PMID:31750298).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, containing 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Integrin alpha 1/CD49a Antibody (CL7207) - BSA Free

• CD49 antigen-like family member A
• CD49a antigen
• CD49a
• Integrin alpha 1
• integrin, alpha 1
• ITGA1
• Laminin and collagen receptor
• very late activation protein 1
• VLA-1
• VLA1integrin alpha-1

Background

Integrin alpha 1 encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 1/CD49a Antibody (NBP2-76478)(1)

Publication using NBP2-76478

• Wu D, Witt RL, Harrington DA, Farach-Carson MC Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated alpha1 beta1 Integrin-Mediated Motility Front Cell Dev Biol 2019-10-16 [PMID: 31750298] (B/N, Human)

Bioinformatics

• Gene Symbol: ITGA1