iNOS Antibody (2D2-B2) [Unconjugated] Summary
Recombinant human iNOS
Accession # P35228
Detects human iNOS. By using synthetic peptides, the epitope recognized by this antibody has been mapped to aa 781-798 of human iNOS. The corresponding sequence of mouse iNOS is identical.
Protein A or G purified from ascites
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1 ug/mL
- Simple Western 10 ug/mL
- Immunohistochemistry 8-25 ug/mL
Packaging, Storage & Formulations
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for iNOS Antibody (2D2-B2) [Unconjugated]
- EC 220.127.116.11
- Hepatocyte NOS
- Inducible NO synthase
- Inducible NOS
- nitric oxide synthase 2, inducible
- nitric oxide synthase 2A (inducible, hepatocytes)
- nitric oxide synthase, inducible
- nitric oxide synthase, macrophage
- NOS type II
- Peptidyl-cysteine S-nitrosylase NOS2
Inducible nitric oxide synthase (iNOS)
is a widely expressed, approximately 130 kDa enzyme that catalyzes the
conversion of L-arginine to nitric oxide (NO) plus citrulline. NO is a
bioactive mediator that plays an important role in hemodynamics by inducing
vascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney.
In other tissues, it is inducibly expressed during inflammation, oxidative
stress, and hyperglycemia. iNOS activity is elevated in a variety of disease
states including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acute
kidney injury, TNF-alpha induced muscle wasting, and the increased
radiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOS
shares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
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FAQs for iNOS Antibody (MAB9502). (Showing 1 - 1 of 1 FAQs).
We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
- We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
Other Available Formats
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Bioinformatics Tool for iNOS Antibody (MAB9502)
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