iNOS Antibody (2D2-B2) [Unconjugated]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clone
2D2-B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

iNOS Antibody (2D2-B2) [Unconjugated] Summary

Immunogen
Recombinant human iNOS
Pro781-His798
Accession # P35228
Specificity
Detects human iNOS. By using synthetic peptides, the epitope recognized by this antibody has been mapped to aa 781-798 of human iNOS. The corresponding sequence of mouse iNOS is identical.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NOS2
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 3 Reviews rated 3
using
MAB9502 in the following applications:

Publications
Read Publications using
MAB9502 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for iNOS Antibody (2D2-B2) [Unconjugated]

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2

Background

Inducible nitric oxide synthase (iNOS) is a widely expressed, approximately 130 kDa enzyme that catalyzes the conversion of L-arginine to nitric oxide (NO) plus citrulline. NO is a bioactive mediator that plays an important role in hemodynamics by inducing vascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney. In other tissues, it is inducibly expressed during inflammation, oxidative stress, and hyperglycemia. iNOS activity is elevated in a variety of disease states including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acute kidney injury, TNF-alpha induced muscle wasting, and the increased radiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOS shares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, IHC

Publications for iNOS Antibody (MAB9502)(13)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Rabbit, Xenograft.

We have publications tested in 4 applications: ICC, IHC, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC
(2)
IHC-P
(2)
Western Blot
(8)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
Rat
(2)
N/A
(1)
Rabbit
(2)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using MAB9502 Applications Species
C Sousa, AJ Leitão, BM Neves, F Judas, C Cavaleiro, AF Mendes Standardised comparison of limonene-derived monoterpenes identifies structural determinants of anti-inflammatory activity Sci Rep, 2020;10(1):7199. 2020 [PMID: 32350292] (Western Blot, Human) Western Blot Human
CP Faria, BM Neves, Á Lourenço, MT Cruz, JD Martins, A Silva, S Pereira, MDC Sousa Giardia lamblia Decreases NF-&kappaB p65RelA Protein Levels and Modulates LPS-Induced Pro-Inflammatory Response in Macrophages Sci Rep, 2020;10(1):6234. 2020 [PMID: 32277133] (Western Blot, N/A) Western Blot N/A
MAM Simões, DCGA Pinto, BMR Neves, AMS Silva Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes Molecules, 2020;25(4):. 2020 [PMID: 32069907] (Western Blot, Mouse) Western Blot Mouse
C Ma, L Wu, L Song, Y He, S Adel Abdo, S Yan, K Sheng, H Wu, J Ran, L Wu The pro-inflammatory effect of NR4A3 in osteoarthritis J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 31701670] (Western Blot, Rat) Western Blot Rat
W Ren, J Hou, C Yang, H Wang, S Wu, Y Wu, X Zhao, C Lu Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res., 2019;38(1):62. 2019 [PMID: 30736829] (Western Blot, Xenograft) Western Blot Xenograft
S Altan, Z O?urtan Dimethyl sulfoxide but not indomethacin is efficient for healing in hydrofluoric acid eye burns Burns, 2017;43(1):232-244. 2017 [PMID: 28341026] (IHC, Rabbit) IHC Rabbit
Yasuda Y, Fujita M, Koike E, Obata K, Shiota M, Kotani Y, Musha T, Tsuji-Kawahara S, Satou T, Masuda S, Okano J, Yamasaki H, Okumoto K, Uesugi T, Nakao S, Hoshiai H, Mandai M Erythropoietin Receptor Antagonist Suppressed Ectopic Hemoglobin Synthesis in Xenografts of HeLa Cells to Promote Their Destruction. PLoS ONE, 2015;10(4):e0122458. 2015 [PMID: 25874769] (IHC, Human) IHC Human
Xia N, Pautz A, Wollscheid U, Reifenberg G, Forstermann U, Li H Artichoke, cynarin and cyanidin downregulate the expression of inducible nitric oxide synthase in human coronary smooth muscle cells. Molecules, 2014;19(3):3654-68. 2014 [PMID: 24662080] (Western Blot, Human) Western Blot Human
Dietrich S, Okun J, Schmidt K, Falk C, Wagner A, Karamustafa S, Radujkovic A, Hegenbart U, Ho A, Dreger P, Luft T High pre-transplant serum nitrate levels predict risk of acute steroid-refractory graft-versus-host disease in the absence of statin therapy. Haematologica, 2014;99(3):541-7. 2014 [PMID: 24142995] (Western Blot, Human) Western Blot Human
Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation. BMC Neurosci, 2012;13(0):50. 2012 [PMID: 22621398] (Western Blot, Rat) Western Blot Rat
Show All 13 Publications.

Reviews for iNOS Antibody (MAB9502) (3) 33

Average Rating: 3
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using MAB9502:
Filter by Applications
Simple Western
(1)
WB
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western iNOS MAB9502
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1
reviewed by:
Lenka Uherková
Simple Western Rat 02/12/2020
View

Summary

ApplicationSimple Western
Sample Testedskin wound - diabetic
SpeciesRat
LotCRF0819031

Comments

CommentsLow Rating Note: Novus Innovators Program - new species or application used on a primary antibody.
  4
reviewed by:
Anonymous
WB Mouse 02/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21075192
SpeciesMouse

Blocking

Blocking DetailsSee PMID 21075192

Details

Detection NotesSee PMID 21075192

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 21075192
  4
reviewed by:
Anonymous
IHC-P Human 02/25/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23147558
SpeciesHuman
Special ApplicationsIndependent review from antibodyresource.comPublished in PMID 23147558.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for iNOS Antibody (MAB9502). (Showing 1 - 1 of 1 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

Secondary Antibodies

 

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Additional iNOS Products

Bioinformatics Tool for iNOS Antibody (MAB9502)

Discover related pathways, diseases and genes to iNOS Antibody (MAB9502). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for iNOS Antibody (MAB9502)

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Pathways for iNOS Antibody (MAB9502)

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PTMs for iNOS Antibody (MAB9502)

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews

3
5
0
4
2
3
0
2
0
1
1

Lenka Uherková
02/12/2020
Application: Simple Western
Species: Rat

 
Anonymous
02/25/2015
Application: WB
Species: Mouse

 
Anonymous
02/25/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol NOS2
Uniprot