iNOS Antibody (2D2-B2) [Alexa Fluor® 405]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 2D2-B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

iNOS Antibody (2D2-B2) [Alexa Fluor® 405] Summary

• Immunogen: Recombinant human iNOS
  Pro781-His798
  Accession # P35228
• Specificity: Detects human iNOS. By using synthetic peptides, the epitope recognized by this antibody has been mapped to aa 781-798 of human iNOS. The corresponding sequence of mouse iNOS is identical.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for iNOS Antibody (2D2-B2) [Alexa Fluor® 405]

• EC 1.14.13.39
• Hepatocyte NOS
• HEP-NOS
• Inducible NO synthase
• Inducible NOS
• iNOS
• nitric oxide synthase 2, inducible
• nitric oxide synthase 2A (inducible, hepatocytes)
• nitric oxide synthase, inducible
• nitric oxide synthase, macrophage
• NOS type II
• NOS
• NOS2
• NOS2A
• Peptidyl-cysteine S-nitrosylase NOS2

Background

Inducible nitric oxide synthase (iNOS) is a widely expressed, approximately 130 kDa enzyme that catalyzes the conversion of L-arginine to nitric oxide (NO) plus citrulline. NO is a bioactive mediator that plays an important role in hemodynamics by inducing vascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney. In other tissues, it is inducibly expressed during inflammation, oxidative stress, and hyperglycemia. iNOS activity is elevated in a variety of disease states including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acute kidney injury, TNF-alpha induced muscle wasting, and the increased radiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOS shares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for iNOS Antibody (FAB9502V). (Showing 1 - 1 of 1 FAQ).

1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
   • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.