Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F)

Images

 
Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 by WB of HeLa treated with IFN-gamma (+) or mock treated (-), HEK293T, HDLM-2, Hep-G2, IDO2 over-expressing HEK, ...read more
Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 (red) in FFPE skin carcinoma by IHC-IF. Antibody: Rabbit anti-IDO1 recombinant ...read more
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 in FFPE tonsil by IHC. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404). ...read more
Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 in FFPE A-549 cells untreated (left) or treated with hIFN-gamma (right) by ICC. ...read more
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 in FFPE colon carcinoma by IHC. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] ...read more
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
BLR031F
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein representing Human IDO1
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IDO1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F)

  • 3dioxygenase
  • EC 1.13.11.52
  • IDO
  • IDO1
  • IDOIDO-1
  • INDO
  • INDOindole 2,3-dioxygenase
  • Indoleamine 2
  • indoleamine 2,3-dioxygenase 1
  • Indoleamine 2,3-dioxygenase
  • indoleamine-pyrrole 2,3 dioxygenase
  • Indoleamine-pyrrole 2,3-dioxygenase

Background

Indoleamine 2,3-dioxygenase (IDO1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. IDO1 acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IDO1