Genetic Strategies: Knockout Validated: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot. The total protein (20 ug) of the dentate gyrus, extracted ...read more
Western Blot: TDO2 Antibody [H00006999-B01P] - Analysis of TDO2 expression in transfected 293T cell line by TDO2 polyclonal antibody. Lane 1: TDO2 transfected lysate(44.66 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. In the cerebellum, the immunoreactivity for TDO was observed in ...read more
Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by immunofluorescent staining. The hippocampal sections of wild-type and Tdo-/- mice were stained ...read more
Western Blot: TDO2 Antibody [H00006999-B01P] - Analysis of TDO2 expression in human liver.
Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. A TDO was not stained in the SVZ. The immunoreactivities for TDO in ...read more
Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. TDO was not stained in the olfactory bulb (OB). The ...read more
Mouse reactivity reported in scientific literature (PMID: 27190010). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.4)
Quality control test: Antibody reactive against mammalian transfected lysate.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for TDO2 Antibody
Tryptophan 2,3-dioxygenase (EC 188.8.131.52) plays a role in catalyzing the first and rat-limiting step in the kynurenine pathway, the major pathway of tryptophan metabolism.[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for TDO2 Antibody (H00006999-B01P). (Showing 1 - 1 of 1 FAQs).
Why do you guys see two different molecular weight bands, one around 46 kDa in HEK cell lysates and the other around 50 kDa in liver lysates?
From the data I have available, the reason you see the molecular weight running slightly lower in the HEK lysate is because these cells were transfected with a partial length protein (a few aa short of the full length). The liver lysate will show you the correct sizing of the endogenous expression. The theoretical molecular weight of this protein is ~48kDa. However the observed weight can vary slightly due to your experimental conditions.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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