IL18R1 Antibody (BLR172J)

Western Blot: IL18R1 Antibody (BLR172J) [NBP3-14745] - Whole cell lysate (25 ug) from NK-92, HEK293T, HDLM-2,NCI-H226, and NCI-H460 cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-IL18R1 recombinantmonoclonal antibody [BLR172J] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 75 seconds. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody [BLR057F].

Flow Cytometry: IL18R1 Antibody (BLR172J) [NBP3-14745] - Rabbit anti-IL18R1 recombinant monoclonal [BLR172J] or isotype control(unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: BLR172J
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IL18R1 Antibody (BLR172J) Summary

• Description: Novus Biologicals Rabbit IL18R1 Antibody (BLR172J) (NBP3-14745) is a recombinant monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: residues 25-329 (ECD)
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IL18R1
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for IL18R1 Antibody (BLR172J)

• CD218 antigen-like family member A
• CD218a antigen
• CD218a
• CDw218a
• cytokine receptor
• IL-18R1
• IL-18R-1
• IL1R-rp
• IL1RRPIL1 receptor-related protein
• IL-1RrpIL18RA
• interleukin 18 receptor 1
• interleukin-18 receptor 1

Background

IL-18 receptor is composed of an alpha and a beta subunit that combine to form a high affinity receptor for IL-18. IL-18 receptor alpha chain, also known as CDw218a, is a 75-80 kD type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of CDw218a on lymphocytes is upregulated after activation. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. The H44 antibody is specific for IL-18 receptor alpha chain and has been reported to block IL-18 binding to the receptor and to be useful for the identification of IL-18 receptor by flow cytometry and IHC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL18R1