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Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)

Order Details

IL-9 ELISA Kit Summary

Standard Curve Range
3.1 - 200 pg/mL
0.5 pg/mL
Kit Type

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.


Application Notes
The human IL-9 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-9

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-9
  2. Biotin-Conjugate anti-human IL-9 monoclonal antibody
  3. Streptavidin-HRP
  4. Human IL-9 Standard lyophilized, 200 pg/ml upon reconstitution
  5. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  6. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  7. Sample Diluent
  8. 1 bottle (5 ml) Calibrator Diluent
  9. Substrate Solution (tetramethyl-benzidine)
  10. Stop Solution (1M Phosphoric acid)
  11. Adhesive Films

Reactivity Notes



Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.

Alternate Names for IL-9 ELISA Kit

  • Cytokine P40
  • HP40
  • IL9
  • IL-9homolog of mouse T cell and mast cell growth factor 40
  • interleukin 9
  • interleukin-9
  • p40 cytokine
  • p40 T-cell and mast cell growth factor
  • P40
  • T-cell growth factor P40


IL-9 is a proinflammatory cytokine historically believed to be involved in type 2 immune responses. However, recent evidence suggests IL-9 may be secreted by other T Helper lineages such as Treg and Th17 in addition to a new category called Th9. This Th9 lineage can either be derived from Th2 cells with TGF-beta or differentiated directly from naive CD4+ T cells with TGF-beta and IL-4. The IL-9 expression in subsets such as Treg and Th17 illustrates the plasticity of cells to reprogram to alternative fates. IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is an extensively glycosylated protein of 14 kDa containing ten cysteine residues involved in disulfide bonding. The human gene maps to chromosome 5q31-32 which is a chromosomal region sometimes deleted in patients with myelodysplastic syndrome. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils, IgE production and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. IL-9 is expressed by Reed-Sternberg cells and Hodgkin lymphoma cells and some large aplastic lymphoma cells, while non-Hodgkin lymphomas and peripheral T-cell lymphomas do not express it.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs


Gene Symbol IL9

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