Human IL-9 ELISA Kit (Colorimetric)

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Sandwich ELISA: Human IL-9 ELISA Kit (Colorimetric) [NBP1-84825] - Representative standard curve for human IL-9 ELISA. Human IL-9 was diluted in serial 2-fold steps in CalibratorDiluent. Do not use this standard curve ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
3.1 - 200 pg/mL
Sensitivity
0.5 pg/mL

Order Details

Human IL-9 ELISA Kit (Colorimetric) Summary

Specificity
The assay detects both natural and recombinant human IL-9. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking human IL-2, IL-4, IL-15, IL-21 proteins at physiologically relevant concentrations into a human IL-9 positive sample. There was no cross reactivity or interference detected.
Standard Curve Range
3.1 - 200 pg/mL
Sensitivity
0.5 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
7.4%
Intra-Assay
7.2%
Spike Recovery
Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL9

Applications/Dilutions

Application Notes
The human IL-9 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-9

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Sample Diluent
  2. Stop Solution (1M Phosphoric acid)
  3. Substrate Solution (tetramethyl-benzidine)
  4. Streptavidin-HRP
  5. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  6. Human IL-9 Standard lyophilized, 200 pg/ml upon reconstitution
  7. Biotin-Conjugate anti-human IL-9 monoclonal antibody
  8. 1 bottle (5 ml) Calibrator Diluent
  9. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  10. Adhesive Films
  11. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-9

Alternate Names for Human IL-9 ELISA Kit (Colorimetric)

  • Cytokine P40
  • HP40
  • IL9
  • IL-9
  • IL-9homolog of mouse T cell and mast cell growth factor 40
  • interleukin 9
  • interleukin-9
  • p40 cytokine
  • p40 T-cell and mast cell growth factor
  • P40
  • T-cell growth factor P40

Background

IL-9 is a proinflammatory cytokine historically believed to be involved in type 2 immune responses. However, recent evidence suggests IL-9 may be secreted by other T Helper lineages such as Treg and Th17 in addition to a new category called Th9. This Th9 lineage can either be derived from Th2 cells with TGF-beta or differentiated directly from naive CD4+ T cells with TGF-beta and IL-4. The IL-9 expression in subsets such as Treg and Th17 illustrates the plasticity of cells to reprogram to alternative fates. IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is an extensively glycosylated protein of 14 kDa containing ten cysteine residues involved in disulfide bonding. The human gene maps to chromosome 5q31-32 which is a chromosomal region sometimes deleted in patients with myelodysplastic syndrome. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils, IgE production and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. IL-9 is expressed by Reed-Sternberg cells and Hodgkin lymphoma cells and some large aplastic lymphoma cells, while non-Hodgkin lymphomas and peripheral T-cell lymphomas do not express it.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL9
Entrez