IL-9 ELISA Kit Summary
|Standard Curve Range
3.1 - 200 pg/mL
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
The human IL-9 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-9
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-9
- Biotin-Conjugate anti-human IL-9 monoclonal antibody
- Human IL-9 Standard lyophilized, 200 pg/ml upon reconstitution
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Sample Diluent
- 1 bottle (5 ml) Calibrator Diluent
- Substrate Solution (tetramethyl-benzidine)
- Stop Solution (1M Phosphoric acid)
- Adhesive Films
Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.
Alternate Names for IL-9 ELISA Kit
- Cytokine P40
- IL-9homolog of mouse T cell and mast cell growth factor 40
- interleukin 9
- p40 cytokine
- p40 T-cell and mast cell growth factor
- T-cell growth factor P40
IL-9 is a proinflammatory cytokine historically believed to be involved in type 2 immune responses. However, recent evidence suggests IL-9 may be secreted by other T Helper lineages such as Treg and Th17 in addition to a new category called Th9. This Th9 lineage can either be derived from Th2 cells with TGF-beta or differentiated directly from naive CD4+ T cells with TGF-beta and IL-4. The IL-9 expression in subsets such as Treg and Th17 illustrates the plasticity of cells to reprogram to alternative fates. IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is an extensively glycosylated protein of 14 kDa containing ten cysteine residues involved in disulfide bonding. The human gene maps to chromosome 5q31-32 which is a chromosomal region sometimes deleted in patients with myelodysplastic syndrome. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils, IgE production and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. IL-9 is expressed by Reed-Sternberg cells and Hodgkin lymphoma cells and some large aplastic lymphoma cells, while non-Hodgkin lymphomas and peripheral T-cell lymphomas do not express it.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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