Human IL-9 ELISA Kit (Colorimetric)

Sandwich ELISA: Human IL-9 ELISA Kit (Colorimetric) [NBP1-84825] - Representative standard curve for human IL-9 ELISA. Human IL-9 was diluted in serial 2-fold steps in CalibratorDiluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Product Discontinued

Human IL-9 ELISA Kit (Colorimetric) Summary

• Specificity: The assay detects both natural and recombinant human IL-9. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking human IL-2, IL-4, IL-15, IL-21 proteins at physiologically relevant concentrations into a human IL-9 positive sample. There was no cross reactivity or interference detected.
• Standard Curve Range: 3.1 - 200 pg/mL
• Sensitivity: 0.5 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.4%
• Intra-Assay: CV% < 7.2%
• Spike Recovery: Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL9

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: The human IL-9 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-9

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-9 ELISA Kit (Colorimetric)

• Cytokine P40
• HP40
• IL9
• IL-9
• IL-9homolog of mouse T cell and mast cell growth factor 40
• interleukin 9
• interleukin-9
• p40 cytokine
• p40 T-cell and mast cell growth factor
• P40
• T-cell growth factor P40

Background

IL-9 is a proinflammatory cytokine historically believed to be involved in type 2 immune responses. However, recent evidence suggests IL-9 may be secreted by other T Helper lineages such as Treg and Th17 in addition to a new category called Th9. This Th9 lineage can either be derived from Th2 cells with TGF-beta or differentiated directly from naive CD4+ T cells with TGF-beta and IL-4. The IL-9 expression in subsets such as Treg and Th17 illustrates the plasticity of cells to reprogram to alternative fates. IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is an extensively glycosylated protein of 14 kDa containing ten cysteine residues involved in disulfide bonding. The human gene maps to chromosome 5q31-32 which is a chromosomal region sometimes deleted in patients with myelodysplastic syndrome. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils, IgE production and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. IL-9 is expressed by Reed-Sternberg cells and Hodgkin lymphoma cells and some large aplastic lymphoma cells, while non-Hodgkin lymphomas and peripheral T-cell lymphomas do not express it.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-9 ELISA Kit (NBP1-84825). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL9