IL-7R alpha/CD127 Antibody (S08-5I2)

Western Blot: IL-7R alpha/CD127 Antibody (S08-5I2) [NBP3-20032] - Western blot detection of IL-7R alpha/CD127 in K562 cell lysates using NBP3-20032 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 70kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S08-5I2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

IL-7R alpha/CD127 Antibody (S08-5I2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human IL-7R alpha/CD127 (Uniprot # P16871)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IL7R
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000-1/5000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for IL-7R alpha/CD127 Antibody (S08-5I2)

• CD127 antigen
• CD127
• CD127ILRA
• CDW127
• IL-7 R alpha
• IL-7 receptor subunit alpha
• IL7R alpha
• IL-7R alpha
• IL-7R subunit alpha
• IL7R
• IL7RA
• IL-7Ra
• IL-7R-alpha
• interleukin 7 receptor alpha chain
• interleukin 7 receptor isoform H5-6
• interleukin 7 receptor
• interleukin-7 receptor subunit alpha

Background

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL7R