Recombinant Human IL-7 Protein Summary
| Description |
Recombinant bioactive protein containing 153 amino acids for Human IL-7 Source:E. coli Amino Acid Sequence:(P13232 ) |
Preparation Method |
Determined to be >98% pure by SDS-PAGE |
| Details of Functionality |
The ED50 as determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is <0.5 ng/ml, corresponding to a specific activity of >2 x 106 units/mg. |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
IL7 |
| Purity |
>98%, by SDS-PAGE |
| Endotoxin Note |
<0.1 ng/ug |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
17.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -20C. |
| Buffer |
Lyophilized from 10 mM Acetic acid. |
| Concentration |
Lyoph |
| Purity |
>98%, by SDS-PAGE |
| Reconstitution Instructions |
Reconstitute in H2O to a concentration of 0.1 mg/ml. The solution can be diluted into other buffered solutions or stored at -20C for long-term storage. |
Alternate Names for Recombinant Human IL-7 Protein
Background
IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant human IL-7 is a 17.4 kDa protein containing 153 amino acid residues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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